Subcellular Proteomics Analysis of Immortalized Cervical Cell and Cervical Cancer Cell_West China Medical Journal

Authors：

 SONGDa-ping ¹ , LüYu-yu ² ,  ZHAOYong ³

1. Department of Pathology, the Third People's Hospital of Mianyang, Mianyang, Sichuan 621000, P. R. China;
2. Department of Pathology, the Fifth People's Hospital of Chongqing, Chongqing 400016, P. R. China;
3. Staff Room of Pathology, Chongqing Medical University, Chongqing 400016, P. R. China;

Corresponding?author：

ZHAOYong, Email: blys01@163.com

Keywords：

Human immortalized endocervical cell; Cervical cancer cell; Proteomics; Two-dimensional gel electrophoresis; Subcellular protein

DOI：

10.7507/1002-0179.20140014

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Objective To establish two-dimensional electrophoresis profiles with high resolution and reproducibility from subcellular immortalized human endocervical cell (H8) and cervical cancer cell (Caski), and to identify the differential expressions of subcellular proteins (cytoplasmic, membranous and nuclear proteins). Methods H8 cells and Caski cells were incubated, and subcellular proteins of H8 cells and Caski cells were extracted and separated by means of immobilized pH gradient-based two-dimensional gel electrophoresis (2-DE). Then the selected differential protein spots were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and SWISS-PROT database. Results We obtained well-resolved, reproducible 2-DE patterns; 6 differentially expressed cytoplasmic proteins and 3 differentially expressed membranous proteins and 9 differentially expressed nuclear proteins were defined in 2-DE gels. Conclusion Subcellular proteins of cervical precancerous lesion and cervical cancer are separated and analyzed by means of 2-DE and MALDI-TOF-MS/MS. There are significant differences between H8 cells and Caski cells. These data may be valuable for research of cervical precancerous lesion and cervical cancer, or as diagnostic markers and therapeutic targets for cervical cancer.

Citation： SONGDa-ping, LüYu-yu, ZHAOYong. Subcellular Proteomics Analysis of Immortalized Cervical Cell and Cervical Cancer Cell. West China Medical Journal, 2014, 29(1): 51-53. doi: 10.7507/1002-0179.20140014 Copy

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1. David E. Two-dimensional gel electrophoresis:an overview[J].Trends in Analyt Chem, 2003, 5(22):263-272,.
2. 李茂玉. 亞細胞蛋白質組學研究[J]. 中國醫師雜志, 2004, 5(2):718-719.
3. 張麗萍, 葉能勝. 宮頸癌蛋白質組學研究新進展[J]. 醫學綜述, 2011, 17(18):2754-2756.
4. Haie-Meder C, de Crevoisier R, Bruna A, et al. Concomitant chemoradiation in patients with cervix cancer[J]. Bull Cancer, 2005, 92(12):1032-1038.
5. Gorg A, Obermaier C, Boguth G, et al. The current state of two dimensional electrophoresis with immobilized pH gradients[J]. Electrophoresis, 2000, 21(6):1037-1053.
6. 宋艷萍, 姜穎, 賀福初. 亞細胞蛋白質組研究進展[J]. 生命科學, 2007, 19(1):104-108.
7. Trejo-Becerril C, Pérez-Cárdenas E, Taja-Chayeb L, et al. Membrane proteins in neoplassic and normal uterine cervix[J]. J Exp Cline Cancer Res, 2001, 20(2):231-237.
8. 林英姿, 陳仁, 楊文, 等. 人宮頸癌組織及癌旁正常組織蛋白質組的比較研究[J]. 第四軍醫大學學報, 2007, 28(17):1593-1596.
9. 蔡思娜, 劉國炳, 郭曉紅, 等. 應用蛋白芯片-飛行質譜技術篩選宮頸癌血清差異蛋白質[J]. 南方醫科大學學報, 2009, 29(1):32-35.
10. 羅俊華, 高春芳, 魏蓉, 等. 宮頸浸潤癌患者血清蛋白質組的特征研究[J]. 解放軍醫學雜志, 2010, 35(4):433-435.
11. 曾亮, 朱紅, 鄧亞平, 等. 應用蛋白質組學技術篩選宮頸癌臨床分期相關蛋白[J]. 現代生物醫學進展, 2009, 8(16):3049-3052.

West China Medical Journal

Subcellular Proteomics Analysis of Immortalized Cervical Cell and Cervical Cancer Cell

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