Objective To investigate the effect of survivin antisense oligodeoxyribonucleotides (survivin ASODNs) on intimal hyperplasia (IH) in vein graft in rats. Methods Autogenous vein graft models were established in 60 Wistar rats by transplanting the interior jugular vein to the common jugular artery using microsurgical technique. The rats were divided into 5 groups according to random digits table, including survivin ASODNs 50 μg group and 200 μg group, scramble ODNs 200 μg group (ODNs group）, Lipofectin+pluronic group and control group. Vein graft samples were collected on 7 d and 14 d after transplantation, respectively. The degrees of hyperplasia were determined and then compared by histomorphology between different groups. The expression of survivin mRNA was measured by RT-PCR and immunohistochemistry. The relevant protein products were detected by Western blot and immunohistochemistry was also used to detect the expression of PCNA. Apoptosis of VSMC was measured by TUNEL.Results Day 7 and 14 were the days that intimal hyperplasied most in control group, ODNs group and Lipofectin+pluronic group, there was no significant difference among these groups yet (Pgt;0.05). The IH could be suppressed by locally transfecting 50 μg of survivin ASODNs (P＜0.05), and it showed a better inhibiting effect in 200 μg of survivin ASODNs group (P＜0.05). The expression of survivin mRNA increased significantly in control group. The expressions of both survivin and PCNA in VSMC significantly decreased in survivin ASODNs group (P＜0.05), whereas the positive cells of TUNEL increased significantly (P＜0.05). Conclusion Transfection of survivin ASODNs may inhibit the IH after vein graft through suppressing the hyperplasia and stimulating the apoptosis of VSMC, and inhibiting the expression of survivin.
目的 調查汶川地震災區中學生腸易激綜合征（IBS）的患病情況，分析羅馬Ⅱ和羅馬Ⅲ診斷標準對該人群IBS患病率的影響。 方法 在汶川地震后2年半和3年，分別用羅馬Ⅱ和羅馬Ⅲ標準制定IBS中學生問卷調查表對地震災區和非地震災區5所中學的中學生進行2次調查，分析比較IBS患病率的變化。 結果 用羅馬Ⅱ標準調查發現地震災區中學生IBS患病率為23.6%；非地震災區患病率為21.6%，二者比較無統計學意義（P=0.267）；用羅馬Ⅲ標準調查發現地震災區中學生IBS患病率為6.2%，非地震災區患病率為4.6%，二者比較無統計學意義（P=0.139）。符合兩種診斷標準的地震災區IBS學生有10.3%，非地震災區IBS學生有9.2%，兩者差異無統計學意義（P＞0.05）。②支持兩種診斷標準的癥狀方面，地震災區IBS學生每天排便＞3次或每周排便＜3次等癥狀相比較有統計學意義（P＜0.001）。③按羅馬Ⅲ標準，各亞型構成比IBS-C為30.4%，IBS-D為28.4%，IBS-M為8.8%，IBS-U為32.4%；按羅馬Ⅱ標準，各亞型構成比為IBS-C為28.5%，IBS-D為47.7%，腹瀉和便秘交替型為18.6%，羅馬Ⅲ標準中的IBS-M和IBS-U合為一組與羅馬Ⅱ標準中的腹瀉和便秘交替型的構成比進行比較，二者有統計學意義（P＜0.001）。 結論 羅馬Ⅱ和羅馬Ⅲ兩種標準調查地震災區中學生IBS患病率和分型存在著差異，但兩種標準對地震災區IBS患病率的影響是對等的，患病率和分型的不同是由兩種標準的本身的差異造成，可能更接近羅馬Ⅲ診斷標準。
Objective To investigate the development and significance of the expression of early growth response gene-1 （EGR-1） in autogenous vein graft in rats and detect the role of it in intimal hyperplasia. Methods Autogenous vein graft model was established in 90 Wistar rats, transplanting the right jugular vein to infra renal abdominal aorta by microsurgical technique. The vein graft samples were harvested at hour 1, 2, 6 and 24, day 3, 7,14, 28 and 42 after procedure. Normal vein as control group. Egr-1 mRNA was measured by reverse transcription-PCR and in situ hybridization. Western blot and immunohistochemistry were used to detect the protein expression of Egr-1. Results Intimal hyperplasia reached peak at day 28 after autogenous vein graft surgery. Egr-1 mRNA and Egr-1 protein hadn’t been found in the normal vein. The expressions of Egr-1 mRNA and Egr-1 protein had biphasic changes. By reverse transcription-PCR and in situ hybridization, we found that the level of Egr-1 mRNA rose at 1 hour after graft, the expression of Egr-1 mRNA was (35±7)%. Decline at hour 6， 24 and day 3, the positive rates of Egr-1 mRNA were (8±2)%, (8±6)% and (8±4)% respectively. Reincrease at day 7, a peak at day 28, the positive rate of Egr-1 mRNA was (45±6)% (compared with other phase, P＜0.01). At day 42, the expression of Egr-1 mRNA declined again. Immunohistochemical staining and Western blot revealed Egr-1 protein had expressed at hour 2 early phase, the expression of Egr-1 protein was (30±5)%, and until to hour 6. The level of Egr-1 protein was decrease at hour 24 and day 3, the positive rates were (7±3)% and (7±8)% respectively. A peak at day 28, the positive rate of Egr-1 protein was (40±9)% (compared with other phase, P＜0.01). We found that immu-noreative Egr-1 located vascular smooth muscle cells （VSMCs） and monocytes/macrophages in tunica media at the early phase of day 7 and 14, and in neointimal and medial VSMCs at later phase of day 28. Egr-1 was also present in the endoluminal endothelial cells. Conclusion In autogenous vein graft, Egr-1 plays an important role in the proliferation of VSMCs. Egr-1 may become a new target for the prevention and therapy of intimal hyperplasia, stenosis and emphraxis after vein graft.