Objective To identify the therapeutic targets and molecular mechanisms of Da Chaihu Decoction in the treatment of acute pancreatitis (AP) based on network pharmacology and molecular docking. Methods From March to May 2024, the active compounds and targets of Da Chaihu Decoction were retrieved from the Traditional Chinese Medicine Systems Pharmacology (TCMSP) database, and the targets related to AP were obtained from the GeneCards database. The intersection of these yielded the common targets of Da Chaihu Decoction for AP treatment. The STRING platform was used to construct a protein-protein interaction network, and Cytoscape 3.9.1 software was employed for network topology analysis to identify core targets and compounds. The Metascape platform was applied for gene ontology functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis, with bubble charts generated using Python 3.8 software. Molecular docking was conducted using AutoDock 1.5.6 software to predict binding affinities between core compounds and targets. Results A total of 84 common targets of Da Chaihu Decoction for AP treatment were identified. The core compounds included quercetin, β-sitosterol, kaempferol, luteolin, and baicalein. The key proteins included AKT1, B-cell leukemia/lymphoma 2 (BCL2), Jun proto-oncogene (JUN), interleukin 1 Beta (IL1B), and nuclear factor kappa B subunit 1 (NFKB1), all of which were enriched in pathways such as lipid and atherosclerosis, PI3K-Akt signaling pathway, mitogen-activated protein kinase (MAPK) signaling pathway, tumor necrosis factor (TNF) signaling pathway, and apoptosis. The binding energies of some core compounds with key proteins were below –5.0 kJ/mol. Conclusion Da Chaihu Decoction may exert anti-inflammatory and anti-apoptotic effects in AP by modulating key protein targets, including AKT1, BCL2, JUN, IL1B, and NFKB1, within pathways such as lipid and atherosclerosis, PI3K-Akt signaling, MAPK signaling, TNF signaling, and apoptosis.
目的 探討成年人顱骨表面遮蓋重建（SSD）的理想閾值設定，為利用SSD進行的頭部CT血管成像（CTA）去骨成像提供閾值設定的依據。 方法 選取2012年8月，在成都市第一人民醫院體檢的、同一單位工作的86位體檢對象，進行顱骨平均CT值、腰椎骨質密度、性別及年齡的兩兩對照及多重線性回歸擬合，建立起預估顱骨平均CT值的多重線性回歸方程，計算出18～80歲人群的理論平均顱骨CT值作為SSD的理想閾值。同時收集2012年10月－2013年2月在該院進行頭部CTA檢查的12例患者的相關資料以驗證其可行性。 結果 18～80歲人群的理論平均顱骨CT值約為640 Hu。以該值作為SSD的閾值進行去骨成像，所有患者圖像質量評分均為5分，可視為去骨成功。 結論 在利用理論顱骨平均CT值作為重建閾值的基礎之上，通過簡單的SSD圖像質量再判斷及修正，即可簡便地完成后處理工作，具有可行性。