Objective To review research progress of corneal tissueengineering.Methods The recent articles on corneal tissue engineering focus on source and selection of corneal cells, the effects of growth factors on culture of corneal cells in vitro. The preparation and selection of three-dimensional biomaterial scaffolds and their b and weak points were discussed. Results The corneal tissue engineering cells come from normal human corneal cells. The embryo corneal cell was excellent. Several kinds of growth factors play important roles in culture, growth and proliferation of corneal cell, and incroporated into matrix.Growth factors including basic fibroblast growth factor, keratinocyte growth factor, transforming growth factor β1 and epidermal growth factor was favor to corneal cell. Collagen, chitosan and glycosaninoglycans were chosen as biomaterial scaffolds. Conclusion Human tissue engineering cornea can be reconstructed and transplanted. It has good tissue compatibility and can be used as human corneal equivalents.
Objective To observe the biocompatibility of the acellular corneal stroma materials prepared by three different methods. Methods Three different serial digestion methods were used to produce the acellular corneal stroma materials. The biocompatibility of the materials was investigated by the cell seeding and the materials were implanted into the rabbit corneal stroma layer. Results The cells in the materials 1 and 2 were not decellularized completely. The rabbit corneal fibroblasts died on the materials 1 and 2 after the cell seeding for 3-4 days. An obvious rejection could be observed after the implantation. The cells in material 3 were decellularized completely and the collagen fibers or elastic fibers were reserved integrally，showing a typical three-dimensional net work. The rabbit corneal fibroblasts could expand on the materials in vitro. No obvious rejection could be observed and the materials were gradually absorbed. Conclusion The acellular porcine cornea stroma materials prepared by trypsin-Dnase-Rnase are suitable for reconstruction of the tissue engineered cornea.
As the most important refraction part in the optical system, cornea possesses characteristics which are important parameters in ophthalmology clinical surgery. During the measurement of the cornea in our study, we acquired the corneal data of Orbscan Ⅱ corneal topographer in real time using the Hook technology under Windows, and then took the data into the corneal analysis software. We then further analyzed and calculated the data to obtain individual Q-value of overall corneal 360 semi-meridian. The corneal analysis software took Visual C++6.0 as development environment, used OpenGL graphics technology to draw three-dimensional individual corneal morphological map and the distribution curve of the Q-value, and achieved real-time corneal data query. It could be concluded that the analysis would further extend the function of the corneal topography system, and provide a solid foundation for the further study of automatic screening of corneal diseases.
目的 探討準分子激光上皮瓣下角膜磨鑲術（LASEK）后角膜上皮下霧狀混濁（Haze）的形成原因及防治措施。 方法 2004年2月－2011年12月期間采用LASEK治療近視患者345 例669 只眼，屈光度?3.50～?13.5 D，平均（?6.87 ± 2.92）D；散光?0.50～?4.00 D，平均（?1.83 ± 1.32）D 。根據患者術前屈光等效球鏡度數分為：中度近視組（?3.50～?5.75 D）120只眼，高度近視組（?6.00～?8.75 D）288只眼，超高度近視組（?9.00～?13.50 D） 261只眼。應用綜合措施防治Haze，術中用日夜配戴的高親水性繃帶型角膜接觸鏡覆蓋保護角膜上皮瓣，術畢頻點激素眼液4次，術后使用激素類聯合非甾體類抗炎眼液及降眼壓藥、抗生素眼液；隨訪6～12個月，觀察術后Haze的發生率，并按Fantes（1990）標準分級，分析相關原因。 結果 LASEK術后6個月時0.5級Haze發生率14.65%（98只眼），無1級Haze。各組0.5級Haze發生率分別為中度近視組2.5%（3只眼），高度近視組11.11%（32只眼），超高度近視組24.14%（63只眼）。 結論 LASEK術后Haze的發生與術眼的屈光度呈正比，與角膜上皮瓣的活性和完整性，術后紫外線照射等有關。術后采用激素類聯合非甾體類抗炎眼液及降眼壓藥等綜合防治措施，可減少、減輕Haze的發生，使LASEK技術更安全有效。
【摘要】 目的 探索顯微眼內窺鏡在后段眼內異物取出及玻璃體切除術中的應用。 方法 將2005年7月-2006年3月38例眼內異物患者分為試驗組（15例）及對照組（23例）。試驗組采用顯微眼內窺鏡下完成對外傷性視網膜玻璃體病變等的處理及眼內異物的取出；對照組采用顯微鏡直視下行常規玻璃體切割及眼內異物取出術。 結果 試驗組術后角膜透明度及眼壓恢復、視力提高及視網膜復位等情況均優于對照組；異物取出情況、手術時間和并發癥兩組無差異。 結論 在觀察困難或異物處于極周邊時應用顯微眼內窺鏡，能夠在手術中直接取出眼內各個部位異物，及時發現視網膜裂孔并同時行抗青光眼手術，減少對角膜材料的依賴和對角膜的損傷，為及時、準確和安全地手術提供了條件。【Abstract】 Objective To investigate the application of the intraocular micro-endoscope in extracting intraocular foreign bodies (IOFB) and vitrectomy. Methods A total of 38 patients with IOFB from July 2005 to March 2006 were divided into a trial group (15 patients) and control group (23 patients). The treatment for traumatic retinal vitreous lesions and extraction of IOFB were performed under the intraocular micro-endoscope in the trial group; while the conventional vitrectomy and IOFB extraction were performed under the microscope in the control group. Results The degree of corneal recovery, improvement of intraocular pressure, visual acuity, and retinal reattachment were better in the trial group than that in the control group. There was no difference in removal of IOFB, surgical time and complications between the two groups. Conclusion In patients with intraocular foreign bodies and cloudy cornea or other conditions that made the observation difficult, intraocular micro-endoscope is a useful convenience without relying on donated cornea. The practice made the judge of size, location and the distance more and more accurate through the monitor.
目的 探討聚乙二醇滴眼液對準分子激光上皮下角膜磨鑲術（LASEK）后角膜霧狀混濁（haze）影響的臨床研究。 方法 回顧性分析2009年3月－2010年3月行LASEK的患者45例90只眼的臨床資料。患者屈光度（?4.68 ± 2.30）D（?1.75～?6.50 D），隨機分為聚乙二醇滴眼液組（試驗組）和對照組，術后定期隨訪觀察兩組角膜haze形成、角膜上皮愈合時間、視力恢復情況及屈光度。 結果 術后1、3、6個月，兩組haze形成差異無統計學意義（P＞0.05），試驗組術后第1、3、5天角膜刺激癥狀明顯輕于對照組（P＜0.05），上皮愈合速度較對照組更快（P＜0.05）。 結論 LASEK術后應用聚乙二醇滴眼液雖然能減輕術后患者角膜刺激癥狀,能促進角膜上皮更快愈合，但是不能減輕haze的形成。