Expression of Cytokine-Induced Neutrophil Chemoattractant-1 in Rats with Transfusion-Related Acute Lung Injury_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

LiangYinghong , GongYanjie , LiuJia ,  WeiMing , TuLing , ZhangYihua , YangLu

Department of Transfusion, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450052, China;

Corresponding?author：

WeiMing, Email: gushiweiming@126.com

Keywords：

Transfusion-related acute lung injury; Cytokine-induced neutrophil chemoattractant; Sprague-Dawley rats

DOI：

10.7507/1671-6205.2016089

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Objective To study the expression of cytokine-induced neutrophil chemoattractant-1(CINC-1)in rats with transfusion-related acute lung injury(TRALI),explore its possible role in the pathogenesis of TRALI. Methods Sixty Sprague-Dawley rats were randomly divided into a normal control group with sham operation,a positive control group with ALI induced by intravenous infusion of lipopolysaccharide(5 mg/kg),and a TRALI group treated by intraperitoneal injection of LPS 2h before the transfusion of human plasma (1mL),a LPS control group treated by intraperitoneal injection of LPS 2h before the transfusion of normal saline(1mL).The reverse transcription-polymerase chain (RT-PCR)was used to detect CINC-1 mRNA.The level of CINC-1 in lung tissue homogenate was measured by ELISA.Morphological changes of the lung tissue were observed under light microscope.Myeloperoxidase (MPO)in lung homogenate and wet lung weight to dry lung weight ratio (W/D)were observed.The number of cells and the percentage of polymorphonuclear neutrophil (PMN)in Bronchoalveolar lavage fluid (BALF)were also compared. Results Compared with the normal control group and the LPS control group,the expression of CINC-1 protein and CINC-1 mRNA were increased significantly in lung of the positive control group and the TRALI group(P<0.05).The number of cells and the percentage of PMN in BALF of the TRALI group [(310.63±76.67)×10⁶/L and (33.57±11.51)%] were significantly higher than those in BALF of the normal control group [(101.36±63.83)×10⁶/L and (9.87±3.56)%](P<0.05).Tissue water content and MPO activity in the TRALI group were significantly higher than those in the normal control group (P<0.05). Conclusion Expression of CINC-1 protein and CINC-1 mRNA are increased in the rat lung with TRALI and PMN infiltration in lung tissue,which suggests CINC-1 participate in the process of the PMN and endothelial cell adhesion and may play an important role in the pathogeneses of TRALI.

Citation： LiangYinghong, GongYanjie, LiuJia, WeiMing, TuLing, ZhangYihua, YangLu. Expression of Cytokine-Induced Neutrophil Chemoattractant-1 in Rats with Transfusion-Related Acute Lung Injury. Chinese Journal of Respiratory and Critical Care Medicine, 2016, 15(4): 379-383. doi: 10.7507/1671-6205.2016089 Copy

1.	王麗, 張春民,張金波.檢測輸血前4項傳染性指標對受血者臨床價值與應用.國際檢驗醫學雜志,2010,31:104.
2.	Anne F,Eder-Beth A,Dy-Jaime M,et al.The residual risk of transfusion-related acute lung injury at the American Red Cross (2008-2011):limitations of a predominantly male-donor plasma mitigation strategy.Transfusion,2013,53:1442-1449.
3.	廖刃, 劉進.圍手術期血液管理:由限制性輸血走向個體化輸血.中華醫學雜志,2014,94:481-482.
4.	Hanane EL,Kenz P,Vander L.Transfusion-related acute lung injury.Euro J Anaesth,2014,31:345-350.
5.	Flesch BK,Petershofen EK,Bux J.TRALI-new challenges for histocompatibility and immunogenetics in transfusion medicine.Tissue Antigens,2011,78:1-7.
6.	Muschtcr S,Bcrthold T,Urcinachcr A.Developments in the definition and clinical impact of human neutrophil antigens.Curr Opin Hematol,2011,18:452-160.
7.	李瑋, 謝如鋒,范華驊.中性粒細胞在輸血相關急性肺損傷中的作用.中國輸血雜志,2012,25:1335-1338.
8.	曹瑞旗, 吳文浩,李曉松,等.羅哌卡因胸段硬膜外阻滯對油酸致豬急性肺損傷時PMN及ICAM-1的影響.河北醫藥,2009,25:2709-2711.
9.	李麗瑋, 花紫菱,張慧箴,等.輸血相關急性肺損傷大鼠模型建立及肺組織病理研究.中國輸血雜志,2012,25:1121-1124.
10.	Gilson CR,Kraus TS,Hod EA,et al.A novel mouse model of red blood cell storage and post-transfusion in vivo survival.Transfusion,2009,49:1546-1553.
11.	Nicholson SE,Johnson RA,John G,et al.Transfusion-related acute lung injury in a rat model of trauma-hemorrhage.J Trau Inj,2011,70:466-471.
12.	Mikawa K,Nishina K,Takaa Y,et al.ONO-1714,a nitric oxide synthase inhibitor,attenuated endotoxin-induced acute lung injury in rabbits.Anesth Anolg,2003,97:1751-1755.
13.	Mark R,Looney N,Roubinian O,et al.Prospective study on the clinical course and outcomes in transfusion-related acute lung injury.Crit Care Med,2014,42:1676-1687.
14.	Silliman CC.The two-event model of transfusion-related acute lung injury.Crit Care Med,2006,34(5 Suppl):S124-S131.
15.	Looney MR,Su X,Van Ziffle JA,et al.Neutrophils and their Fcγ receptors are essential in a mouse model of transfusion-related acute lung injury.J Clin Invest,2006,116:1615-1623.
16.	Takahashi D,Fujihara M,Azuma H,et al.Stimulation of human neutrophils with sera containing HLA Class I alloantibody causes preferential degranulation of azurophilic granules and secretory vesicles.Vox Sang,2010,98:560-566.
17.	Bernadette F,West-Christopher C,Silliman CC.Transfusion-related acute lung injury:advances in understanding the role of proinflammatory mediators in its genesis.Expert Rev Hematol,2013,6:265-276.
18.	Gottschall JL,Triulzi DJ,Curtis B,et al.The frequency and specificity of human neutrophil antigen antibodies in a blood donor population.Transfusion,2011,51:820-827.
19.	Sayah DM,Looney MR, Toy P,et al.Transfusion reactions:newer concepts on the pathophysiology,incidence,treatment,and prevention of transfusion-related acute lung injury.Crit Care Clin,2012,28:363-372.
20.	Silliman CC,Bercovitz RS,Khan SY,et al.Antibodies to the HLA-A2 antigen prime neutrophils and serve as the second event in an in vitro model of transfusion-related acute lung injury.Vox Sanguinis,2014,107:76-82.
21.	Leanne C,Amandeep S,Gregory A,et al.Electronic health record surveillance algorithms facilitate the detection of transfusion-related pulmonary complications.Transfusion,2013,53:1205-1216.
22.	夏長江, 孟革,趙建,等.中性粒細胞在急性肺損傷中的作用機制研究進展.國際藥學研究雜志,2009,36:418-422.
23.	Takaishi K,Ohtsuka T,Tsuneyoshi S,et al.Inhibition of the production of rat cytokine-induced neutrophil chemoattractant (CINC)-1,a member of the interleukin-8 family,by adenovirus-mediated overexpression of IκBα.J Biochem,2000,127:511-516.

1. 王麗, 張春民,張金波.檢測輸血前4項傳染性指標對受血者臨床價值與應用.國際檢驗醫學雜志,2010,31:104.
2. Anne F,Eder-Beth A,Dy-Jaime M,et al.The residual risk of transfusion-related acute lung injury at the American Red Cross (2008-2011):limitations of a predominantly male-donor plasma mitigation strategy.Transfusion,2013,53:1442-1449.
3. 廖刃, 劉進.圍手術期血液管理:由限制性輸血走向個體化輸血.中華醫學雜志,2014,94:481-482.
4. Hanane EL,Kenz P,Vander L.Transfusion-related acute lung injury.Euro J Anaesth,2014,31:345-350.
5. Flesch BK,Petershofen EK,Bux J.TRALI-new challenges for histocompatibility and immunogenetics in transfusion medicine.Tissue Antigens,2011,78:1-7.
6. Muschtcr S,Bcrthold T,Urcinachcr A.Developments in the definition and clinical impact of human neutrophil antigens.Curr Opin Hematol,2011,18:452-160.
7. 李瑋, 謝如鋒,范華驊.中性粒細胞在輸血相關急性肺損傷中的作用.中國輸血雜志,2012,25:1335-1338.
8. 曹瑞旗, 吳文浩,李曉松,等.羅哌卡因胸段硬膜外阻滯對油酸致豬急性肺損傷時PMN及ICAM-1的影響.河北醫藥,2009,25:2709-2711.
9. 李麗瑋, 花紫菱,張慧箴,等.輸血相關急性肺損傷大鼠模型建立及肺組織病理研究.中國輸血雜志,2012,25:1121-1124.
10. Gilson CR,Kraus TS,Hod EA,et al.A novel mouse model of red blood cell storage and post-transfusion in vivo survival.Transfusion,2009,49:1546-1553.
11. Nicholson SE,Johnson RA,John G,et al.Transfusion-related acute lung injury in a rat model of trauma-hemorrhage.J Trau Inj,2011,70:466-471.
12. Mikawa K,Nishina K,Takaa Y,et al.ONO-1714,a nitric oxide synthase inhibitor,attenuated endotoxin-induced acute lung injury in rabbits.Anesth Anolg,2003,97:1751-1755.
13. Mark R,Looney N,Roubinian O,et al.Prospective study on the clinical course and outcomes in transfusion-related acute lung injury.Crit Care Med,2014,42:1676-1687.
14. Silliman CC.The two-event model of transfusion-related acute lung injury.Crit Care Med,2006,34(5 Suppl):S124-S131.
15. Looney MR,Su X,Van Ziffle JA,et al.Neutrophils and their Fcγ receptors are essential in a mouse model of transfusion-related acute lung injury.J Clin Invest,2006,116:1615-1623.
16. Takahashi D,Fujihara M,Azuma H,et al.Stimulation of human neutrophils with sera containing HLA Class I alloantibody causes preferential degranulation of azurophilic granules and secretory vesicles.Vox Sang,2010,98:560-566.
17. Bernadette F,West-Christopher C,Silliman CC.Transfusion-related acute lung injury:advances in understanding the role of proinflammatory mediators in its genesis.Expert Rev Hematol,2013,6:265-276.
18. Gottschall JL,Triulzi DJ,Curtis B,et al.The frequency and specificity of human neutrophil antigen antibodies in a blood donor population.Transfusion,2011,51:820-827.
19. Sayah DM,Looney MR, Toy P,et al.Transfusion reactions:newer concepts on the pathophysiology,incidence,treatment,and prevention of transfusion-related acute lung injury.Crit Care Clin,2012,28:363-372.
20. Silliman CC,Bercovitz RS,Khan SY,et al.Antibodies to the HLA-A2 antigen prime neutrophils and serve as the second event in an in vitro model of transfusion-related acute lung injury.Vox Sanguinis,2014,107:76-82.
21. Leanne C,Amandeep S,Gregory A,et al.Electronic health record surveillance algorithms facilitate the detection of transfusion-related pulmonary complications.Transfusion,2013,53:1205-1216.
22. 夏長江, 孟革,趙建,等.中性粒細胞在急性肺損傷中的作用機制研究進展.國際藥學研究雜志,2009,36:418-422.
23. Takaishi K,Ohtsuka T,Tsuneyoshi S,et al.Inhibition of the production of rat cytokine-induced neutrophil chemoattractant (CINC)-1,a member of the interleukin-8 family,by adenovirus-mediated overexpression of IκBα.J Biochem,2000,127:511-516.

Chinese Journal of Respiratory and Critical Care Medicine

Expression of Cytokine-Induced Neutrophil Chemoattractant-1 in Rats with Transfusion-Related Acute Lung Injury

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