Therapeutic Effect on Radioative Lung Injury by Different Doses of Tanshinone ⅡA Microemulsion_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

SunYunhui ¹ , YangXinlei ¹ , MaXuemei ¹ , YangXiaodong ¹ , PingYang ² , YuLian ² ,  BaoWenhua ¹

1. Department of Respiratory Medicine, School of Jiamusi University, Jiamusi, Heilongjiang, 154002, China;
2. ;

Corresponding?author：

BaoWenhua, Email: baowenhua3@sina.com

Keywords：

Radiation-induced lung injury; Tanshinone ⅡA microemulsion; Dose; Smad

DOI：

10.7507/1671-6205.2016088

Video：

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Objective To investigate the therapeutic effects of different doses of tanshinone ⅡA microemulsion on radioactive lung injury. Methods Seventy-two Wistar rats were randomly divided into a healthy control group,a model group,a liposome microemulsion treatment group,a tanshinone ⅡA microemulsion high-dose group,a tanshinone ⅡA microemulsion middle-dose group,and a tanshinone ⅡA microemulsion low-dose group.Radiation-induced lung injury model was established by irradiation of radiotherapy instrument.In addition to the control group,other groups received 6MV X radiation with one dosage of 22Gy.Four rats in each group were sacrificed on 7^th,14^th,and 28^th day,respectively.Lung tissues were sampled to analyze the pathological changes by HE staining and the Smad7 mRNA expression by RT-PCR.The level of glutathione(GSH)in peripheral blood was determined by ultraviolet spectrophotometric method. Results In the model group and four treatment groups,lung tissue biopsy showed the pathological changes gradually from pulmonary alveolitis to fibrosis.The level of Smad7 mRNA in lung tissue and GSH in peripheral blood were higher in the high-dose group,the middle-dose group and the low-dose group than those in the model group at all time points(P<0.05),and were highest in the high-dose group.There was no significant differences in the level of Smad7 mRNA in lung tissue and GSH in peripheral blood between the liposome microemulsion treatment group and the middle-dose group. Conclusion Tanshinone ⅡA microemulsion has treatment effect on lung injury in a dose dependent manner.

Citation： SunYunhui, YangXinlei, MaXuemei, YangXiaodong, PingYang, YuLian, BaoWenhua. Therapeutic Effect on Radioative Lung Injury by Different Doses of Tanshinone ⅡA Microemulsion. Chinese Journal of Respiratory and Critical Care Medicine, 2016, 15(4): 374-378. doi: 10.7507/1671-6205.2016088 Copy

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2.	史毓杰, 曾抗,李國鋒,等.鬼臼毒素固體脂質納米粒的制備及對人表皮細胞體外增殖的影響.解放軍醫學雜志,2008,33:42-44.
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14.	趙斐. 丹參酮IIA對TGF-β1誘導的HK-2細胞TGF-β1/Smads信號通路影響的實驗研究[學位論文].北京中醫藥大學,2014.
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17.	雷章, 盧宏達,盧馳,等.還原型谷胱甘肽對大鼠急性放射性肺損傷炎癥的影響.中國醫院藥學雜志,2014,34:1449-1453.
18.	徐婷貞, 楊起初,安嬌嬌,等.虎杖對放射性肺損傷大鼠TGF-β1/Smad蛋白的影響.中華中醫藥學刊,2015,33:1421-1425.

1. 毛艷, 劉文其.放射性肺損傷防護的研究進展.腫瘤,2012,32:389-394.
2. 史毓杰, 曾抗,李國鋒,等.鬼臼毒素固體脂質納米粒的制備及對人表皮細胞體外增殖的影響.解放軍醫學雜志,2008,33:42-44.
3. 畢茹. 胰島素固體脂質納米粒粉霧劑的制備.西北藥學雜志,2011,26:204-206.
4. 王新春, 侯世祥,陽長明,等.大黃復方液體噴霧劑中揮發油微乳與相圖的應用.中成藥,2004,26:8-11.
5. 串俊蘭, 龔濤,孫遜,等.利福平固體脂質納米粒的體外細胞攝取研究.華西藥學雜志,2011,26:223-225.
6. 馮欣, 張須學,姬誠.丹參酮亞微乳制劑的制備工藝和質量評價.中國醫藥指南,2012,10:51-53.
7. 叢軍茲, 王丹,周楊,等.丹參酮ⅡA納米結構脂質載體的制備及體外評價.牡丹江醫學院學報,2015,36:27-29.
8. 吳麗明, 黃國紅,李慶國.高效液相色譜法測定吉非替尼的含量.當代醫學,2011,17:23-25.
9. Nakao A,Fujii M,Matsumura R,et al.Transient gene transfer and expression of Smad7 prevents bleomycin induced lung fibrosis in mice.J Clin Invest,1999,104:5-11.
10. 劉雙秀, 郭和宗,馮書鳳,等.丹參酮治療放射性肺炎臨床觀察.中醫學報,2014,29:1411-1412.
11. 李廣虎, 李志平,徐泳,等.丹參酮ⅡA對放射性肺纖維化防治作用的實驗研究.中華放射腫瘤學雜志,2006,15:50-54.
12. 周雨田, 徐軍.反義內皮素轉換酶核酸表達質粒納米粒所致內皮素的減少對哮喘小鼠氣道高反應性的影響.實用醫院臨床雜志,2013,10:28-31.
13. 劉佳. 殼聚糖納米粒包裹反義內皮素轉換酶核酸表達質粒與哮喘氣道高反應性.中國組織工程研究,2015,19:6913-6917.
14. 趙斐. 丹參酮IIA對TGF-β1誘導的HK-2細胞TGF-β1/Smads信號通路影響的實驗研究[學位論文].北京中醫藥大學,2014.
15. 李瑞珊. 卵磷脂注射溶脂的實驗研究[學位論文].南方醫科大學,2010.
16. 陳志霄, 周道安,趙國旗.放射性肺損傷的研究進展.中國腫瘤,2010,19:53-58.
17. 雷章, 盧宏達,盧馳,等.還原型谷胱甘肽對大鼠急性放射性肺損傷炎癥的影響.中國醫院藥學雜志,2014,34:1449-1453.
18. 徐婷貞, 楊起初,安嬌嬌,等.虎杖對放射性肺損傷大鼠TGF-β1/Smad蛋白的影響.中華中醫藥學刊,2015,33:1421-1425.

Chinese Journal of Respiratory and Critical Care Medicine

Therapeutic Effect on Radioative Lung Injury by Different Doses of Tanshinone ⅡA Microemulsion

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