Curcumin can inhibit the lipopolysaccharide-induced up-regulation of endogenousβ-glucoronidase expression_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

WANG Yang ¹ ,  YAO Dianbo ² , WU Shuodong ²

1. Department of Pathology, Shenyang DI’AN Medical Testing Company Limited, Shenyang 110041, P. R. China;
2. Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang 110004, P. R. China;

Corresponding?author：

YAO Dianbo, Email: yaodianbo@163.com

Keywords：

biliary pigment stone; curcumin; lipopolysaccharide; β-glucuronidase; c-myc protein

DOI：

10.7507/1007-9424.201903082

Video：

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Objective To investigate the effect of curcumin on the expression regulation of endogenousβ-glucoronidase (β-GD) induced by lipopolysaccharide (LPS).Methods ① Human normal intrahepatic biliary epithelial cell line (HiBEpiC) cells in the logarithmic growth phase were divided into blank control group (0 h group) and 7 different stimulation time groups. The cell density was adjusted to 1×10⁴/mL, and the cells were stimulated with 100 mg/mL LPS for 1, 3, 6, 18, and 24 hours respectively, including another two groups where the cells were cultured with LPS-free medium for 18 and 24 hours after LPS stimulation for 24 h. ② HiBEpiC cells in the logarithmic growth phase were divided into blank control group, LPS+low, medium, and high concentration curcumin group. The cell density was adjusted to 1×10⁴/mL. In the blank control group, cells were not stimulated with any reagent; in the LPS group, cells were stimulated with 100 mg/mL LPS, in the other three groups, the cells were stimulated with 100 mg/mL LPS and simultaneously 20, 40, and 80 μmol/L curcumin, respectively, for 24 hours. The expressions of c-myc and endogenous β-GD were detected by Western blot method.Results ① The expressions of endogenous β-GD and c-myc in HiBEpiC cells gradually increased with the prolongation of treatment time by LPS, and the expression levels of β-GD and c-myc at each time point group were significantly different from those in the 0 h group (P<0.05). ② There were significant difference between any two groups of the blank control group, LPS group, LPS+low concentration of curcumin group, LPS+medium concentration of curcumin group, and LPS+high concentration of curcumin group (P<0.05).Conclusion Curcumin is able to inhibit the increased expression of endogenous β-GD induced by LPS, possibly via inhibiting expression of c-myc.

Citation： WANG Yang, YAO Dianbo, WU Shuodong. Curcumin can inhibit the lipopolysaccharide-induced up-regulation of endogenousβ-glucoronidase expression. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2019, 26(8): 917-922. doi: 10.7507/1007-9424.201903082 Copy

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9.	Wang JY, Wang X, Wang XJ, et al. Curcumin inhibits the growth via Wnt/β-catenin pathway in non-small-cell lung cancer cells. Eur Rev Med Pharmacol Sci, 2018, 22(21): 7492-7499.
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15.	崔東旭, 孫韶龍, 張小薄, 等. 內毒素調節血細胞釋放β-葡萄糖苷酸酶的實驗研究. 中國現代醫學雜志, 2008, 18(3): 283-285.
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25.	李強, 王斌生, 柴琛. 姜黃素誘導胃癌細胞凋亡中MicroRNA表達譜的分析. 醫藥前沿, 2016, 6(17): 160-162.
26.	周銀霞, 張崇, 傅海珍, 等. 姜黃素類組分通過下調TLR4/NF-κB通路抑制LPS誘導的血管內皮細胞損傷. 中南藥學, 2018, 16(11): 1521-1525.
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29.	Yang Q, Wu Z, Liu F, et al. Effective treatment of chronic proliferative cholangitis by local gentamicin infusion in rabbits. Biomed Res Int, 2018, 2018: 6751952.

1. Tazuma S. Gallstone disease: epidemiology, pathogenesis, and classification of biliary stones (common bile duct and intrahepatic). Best Pract Res Clin Gastroenterol, 2006, 20(6): 1075-1083.
2. 中華醫學會外科學分會膽道外科學組. 肝膽管結石病診斷治療指南. 中華消化外科雜志, 2007, 6(2): 156-161.
3. 吳碩東, 于宏, 蘇洋, 等. 原發性膽管色素結石形成原因的階段性研究總結. 中華肝膽外科雜志, 2007, 13(8): 515-518.
4. Wang Y, Qi M, Qin C, et al. Role of the biliary microbiome in gallstone disease. Expert Rev Gastroenterol Hepatol, 2018, 12(12): 1193-1205.
5. 張西波, 崔乃強, 李東華. 清熱利濕方防治兔膽色素結石形成的實驗研究. 中國中西醫結合雜志, 2007, 27(3): 241-243.
6. 姚殿波, 鄧天麟, 柯昌偉, 等. 內源性β-葡萄糖醛酸酶表達在肝內膽管結石形成中的作用. 中華肝膽外科雜志, 2017, 23(7): 460-463.
7. Notoya M, Nishimura H, Woo JT, et al. Curcumin inhibits the proliferation and mineralization of cultured osteoblasts. Eur J Pharmacol, 2006, 534(1-3): 55-62.
8. Zhu M, Zheng Z, Huang J, et al. Modulation of miR-34a in curcumin-induced antiproliferation of prostate cancer cells. J Cell Biochem, 2019, [Epub ahead of print].
9. Wang JY, Wang X, Wang XJ, et al. Curcumin inhibits the growth via Wnt/β-catenin pathway in non-small-cell lung cancer cells. Eur Rev Med Pharmacol Sci, 2018, 22(21): 7492-7499.
10. Naz H, Islam A, Waheed A, et al. Human β-glucuronidase: structure, function, and application in enzyme replacement therapy. Rejuvenation Res, 2013, 16(5): 352-363.
11. Maki T. Pathogenesis of calcium bilirubinate gallstone: role ofE. coli, beta-glucuronidase and coagulation by inorganic ions, polyelectrolytes and agitation. Ann Surg, 1966, 164(1): 90-100.
12. Ma WJ, Zhou Y, Shrestha A, et al. Applying chemical bile duct embolization to achieve chemical hepatectomy in hepatolithiasis: a further experimental study. J Surg Res, 2014, 187(1): 113-121.
13. Li F, Cheng J, He S, et al. The practical value of applying chemical biliary duct embolization to chemical hepatectomy for treatment of hepatolithiasis. J Surg Res, 2005, 127(2): 131-138.
14. 蘇洋, 吳碩東, 金俊哲, 等. 腸屏障功能對豚鼠膽囊膽色素結石形成的影響. 世界華人消化雜志, 2005, 13(24): 2853-2857.
15. 崔東旭, 孫韶龍, 張小薄, 等. 內毒素調節血細胞釋放β-葡萄糖苷酸酶的實驗研究. 中國現代醫學雜志, 2008, 18(3): 283-285.
16. 孫韶龍, 吳碩東, 戴顯偉, 等. 內毒素調節肝臟β-葡萄糖苷酸酶mRNA的表達. 世界華人消化雜志, 2007, 15(17): 1887-1892.
17. Yao D, Dong Q, Tian Y, et al. Lipopolysaccharide stimulates endogenous β-glucuronidase via PKC/NF-κB/c-myc signaling cascade: a possible factor in hepatolithiasis formation. Mol Cell Biochem, 2018, 444(1-2): 93-102.
18. Soliman MM, Abdo Nassan M, Ismail TA. Immunohistochemical and molecular study on the protective effect of curcumin against hepatic toxicity induced by paracetamol in Wistar rats. BMC Complement Altern Med, 2014, 14: 457.
19. Hao Q, Chen X, Wang X, et al. Curcumin attenuates angiotensin Ⅱ-induced abdominal aortic aneurysm by inhibition of inflammatory response and ERK signaling pathways. Evid Based Complement Alternat Med, 2014, 2014: 270930.
20. Guo LD, Shen YQ, Zhao XH, et al. Curcumin combined with oxaliplatin effectively suppress colorectal carcinoma in vivo through inducing apoptosis. Phytother Res, 2015, 29(3): 357-365.
21. Liu G, Xiang T, Wu QF, et al. Curcumin suppresses the proliferation of gastric cancer cells by downregulating H19. Oncol Lett, 2016, 12(6): 5156-5162.
22. Zheng R, Deng Q, Liu Y, et al. Curcumin inhibits gastric carcinoma cell growth and induces apoptosis by suppressing the Wnt/β-Catenin signaling pathway. Med Sci Monit, 2017, 23: 163-171.
23. Kong YW, Cannell IG, de Moor CH, et al. The mechanism of micro-RNA-mediated translation repression is determined by the promoter of the target gene. Proc Natl Acad Sci U S A, 2008, 105(26): 8866-8871.
24. Li J, Wang K, Chen X, et al. Transcriptional activation of microRNA-34a by NF-kappa B in human esophageal cancer cells. BMC Mol Biol, 2012, 13: 4.
25. 李強, 王斌生, 柴琛. 姜黃素誘導胃癌細胞凋亡中MicroRNA表達譜的分析. 醫藥前沿, 2016, 6(17): 160-162.
26. 周銀霞, 張崇, 傅海珍, 等. 姜黃素類組分通過下調TLR4/NF-κB通路抑制LPS誘導的血管內皮細胞損傷. 中南藥學, 2018, 16(11): 1521-1525.
27. Bandgar BP, Kinkar SN, Chavan HV, et al. Synthesis and biological evaluation of asymmetric indole curcumin analogs as potential anti-inflammatory and antioxidant agents. J Enzyme Inhib Med Chem, 2014, 29(1): 7-11.
28. Yang Q, Wang J, Liu F, et al. A novel rabbit model for benign biliary stricture formation and the effects of medication infusions on stricture formation. Dig Dis Sci, 2018, 63(10): 2653-2661.
29. Yang Q, Wu Z, Liu F, et al. Effective treatment of chronic proliferative cholangitis by local gentamicin infusion in rabbits. Biomed Res Int, 2018, 2018: 6751952.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Curcumin can inhibit the lipopolysaccharide-induced up-regulation of endogenousβ-glucoronidase expression

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