Analysis of drug resistance of staphylococci in Whire Union Bacterial Resistance Surveillance Network across Sichuan from 2015 to 2018_West China Medical Journal

Authors：

WU Xianli ^1,2 , LI Jing ¹ , AO Maocheng ¹ , PANG Zaiyuan ² ,  XIE Yi ¹

1. Department of Experimental Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;
2. Department of Clinical Laboratory, Center Hospital of Panzhihua, Panzhihua, Sichuan 617000, P. R. China;

Corresponding?author：

XIE Yi, Email: xie_yi_77@163.com

Keywords：

Staphylococci; Staphylococcus aureus; Coagulase-negative staphylococci; Methicillin-resistant coagulase-negative staphylococci; Methicillin-resistant Staphylococcus aureus

DOI：

10.7507/1002-0179.201907074

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Objective To analyze the characteristics of distribution and drug resistance of clinical isolated staphylococci in the Whire Union Bacterial Resistance Surveillance Network across Sichuan from 2015 to 2018, so as to provide reference for clinical rational drug use and management of drug-resistant bacteria in Sichuan.Methods A total of 18 023 strains of staphylococci were isolated from 9 hospitals of Whire Union Bacterial Resistance Surveillance Network for four years (2015-2018). Drug susceptibility test was carried out by disk diffusion method or automated instrument method. The data were statistically analyzed by WHONET 5.6 according to CLSI 2016 standard.Results The 18 023 strains of staphylococci included 10 865 (60.28%) Staphylococcus aureus and 7 158 (39.72%) coagulase negative staphylococci. No strains resistant to vancomycin and linezolid were found. The detection rates of methicillin-resistant Staphylococcus aureus and methicillin-resistant coagulase-negative staphylococci were 25.10% (2 727/ 10 865) and 75.60% (5 411/7 158), respectively. The sensitivity of methicillin-resistant staphylococci to most antibiotics was significantly lower than that of methicillin-sensitive strains (P<0.05). The susceptibility rate of staphylococci to some antibiotics was significantly different from 2015 to 2018(P<0.05). The susceptibility rates of Staphylococcus aureus from different samples to rifampicin, moxifloxacin, ciprofloxacin, levofloxacin, oxacillin and erythromycin were significantly different (P<0.05). The susceptibility rates of Staphylococcus aureus from different departments in different samples of sulfamethoxazole, rifampicin, moxifloxacin, ciprofloxacin, levofloxacin, oxacillin, gentamicin, tetracycline, clindamycin and erythromycin were significantly different (P<0.05).Conclusions The susceptibility of strains isolated from different periods, different specimens and departments to the same antimicrobial agents varies greatly. For the infection of staphylococci, we should use drugs under the guidance of drug susceptibility according to the source of samples, which can avoid the abuse of beta-lactam drugs. Strengthening the monitoring and control of drug-resistant bacteria can prevent or reduce the spread of drug-resistant bacteria.

Citation： WU Xianli, LI Jing, AO Maocheng, PANG Zaiyuan, XIE Yi. Analysis of drug resistance of staphylococci in Whire Union Bacterial Resistance Surveillance Network across Sichuan from 2015 to 2018. West China Medical Journal, 2019, 34(8): 900-906. doi: 10.7507/1002-0179.201907074 Copy

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5.	李艷, 楊崇勤. 改進皮膚與瓶蓋消毒方法對血培養凝固酶陰性葡萄球菌檢出率的影響. 中國感染控制雜志, 2016, 15(5): 324-326.
6.	李明友, 林茂銳, 黃基偉, 等. 血培養和血清降鈣素原檢測對血流感染者早期診治的臨床價值. 國際檢驗醫學雜志, 2015, 36(10): 1381-1382.
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10.	方小敏, 李小冰, 郭兆旺. 2014-2015年我院凝固酶陰性葡萄球菌的分布及耐藥性分析. 臨床合理用藥雜志, 2016, 9(10B): 7-8.
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13.	劉青, 樊冰. 兒童呼吸道感染患者金黃色葡萄球菌的分離與耐藥性分析. 現代檢驗醫學雜志, 2016, 31(3): 150-152, 155.
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15.	石秀梅, 朱光發, 王愛萍. 509株金黃色葡萄球菌感染的臨床分布和耐藥性分析. 臨床肺科雜志, 2016, 21(8): 1381-1384.
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19.	孫敏, 徐永成, 苗祖豪. 金黃色葡萄球菌的分布特征及耐藥性分析. 國際檢驗醫學雜志, 2016, 37(16): 2310-2313.
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1. 倪語星, 尚紅. 臨床微生物學檢驗. 5版. 北京: 人民衛生出版社, 2012: 80-81.
2. Clinical and Laboratory Standards Institute. M100S: performance standards for antimicrobial susceptibility testing. 26th Edition, 2016.(2016-01)[2019-07-05]. http://ljzx.cqrmhospital.com/upfiles/201601/20160112155335884.pdf.
3. 胡洪華, 楊永長, 肖代雯, 等. 凝固酶陰性葡萄球菌生物被膜形成及耐藥性分析. 中國感染控制雜志, 2015, 14(11): 721-725.
4. 郭瑞林, 李凱旋, 蘇冰, 等. 新生兒呼吸道分離凝固酶陰性葡萄球菌耐藥性分析. 檢驗醫學與臨床, 2015, 12(3): 322-323, 326.
5. 李艷, 楊崇勤. 改進皮膚與瓶蓋消毒方法對血培養凝固酶陰性葡萄球菌檢出率的影響. 中國感染控制雜志, 2016, 15(5): 324-326.
6. 李明友, 林茂銳, 黃基偉, 等. 血培養和血清降鈣素原檢測對血流感染者早期診治的臨床價值. 國際檢驗醫學雜志, 2015, 36(10): 1381-1382.
7. 黃有平, 李珺. 血培養檢出病原菌分布及雙份血培養法的價值分析. 國際檢驗醫學雜志, 2015, 36(20): 2994-2996.
8. 陳海濱, 單文治, 周光, 等. 骨科病房凝固酶陰性葡萄球菌耐藥性分析. 中國藥業, 2009, 18(9): 59-60.
9. 趙珉, 戴宏, 沈繼錄, 等. 132例腹膜透析相關性腹膜炎病原菌及其耐藥性分析. 中國感染與化療雜志, 2015, 15(3): 236-243.
10. 方小敏, 李小冰, 郭兆旺. 2014-2015年我院凝固酶陰性葡萄球菌的分布及耐藥性分析. 臨床合理用藥雜志, 2016, 9(10B): 7-8.
11. 陽蘋, 夏云. 葡萄球菌感染的臨床分布及耐藥性分析. 重慶醫學, 2012, 41(30): 3167-3168, 3170.
12. 朱德妹, 胡付品, 汪復, 等. 2009年中國CHINET葡萄球菌屬細菌耐藥性監測. 中國感染與化療雜志, 2010, 10(6): 414-420.
13. 劉青, 樊冰. 兒童呼吸道感染患者金黃色葡萄球菌的分離與耐藥性分析. 現代檢驗醫學雜志, 2016, 31(3): 150-152, 155.
14. 胡付品, 郭燕, 朱德妹, 等. 2016年中國CHINET細菌耐藥性監測. 中國感染與化療雜志, 2017, 17(5): 481-491.
15. 石秀梅, 朱光發, 王愛萍. 509株金黃色葡萄球菌感染的臨床分布和耐藥性分析. 臨床肺科雜志, 2016, 21(8): 1381-1384.
16. Sun W, Chen H, Liu Y, et al. Prevalence and characterization of heterogeneous vancomycin-intermediate Staphylococcus aureus isolates from 14 gitics in China. Antimicrob Agents Chemother, 2009, 53(9): 3642-3649.
17. 孫聞嘉. 我國異質性萬古霉素中介的金黃色葡萄球菌（hVISA）的發生率、分子流行病學及病例對照研究. 北京: 中國協和醫科大學, 2008.
18. 王敬華, 周惠琴, 楊歡, 等. 利奈唑胺對h-VRS體外抗菌活性研究. 中國抗生素雜志, 2009, 34(9): 570-574.
19. 孫敏, 徐永成, 苗祖豪. 金黃色葡萄球菌的分布特征及耐藥性分析. 國際檢驗醫學雜志, 2016, 37(16): 2310-2313.
20. 姚明, 關立鋒, 吳銀生, 等. 甲氧西林耐藥金黃色葡萄球菌基因分型及耐藥現狀研究. 中華燒傷雜志, 2014, 30(5): 428-432.

West China Medical Journal

Analysis of drug resistance of staphylococci in Whire Union Bacterial Resistance Surveillance Network across Sichuan from 2015 to 2018

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