Distribution of human papilloma virus subtypes detected by flow fluorescence hybridization in physical examination women in West China Hospital of Sichuan University_West China Medical Journal

Authors：

ZHONG Huiyu , LIU Tangyuheng , WANG Minjin , WU Lijuan , ZHUANG Jie , WANG Jun ,  SONG Xingbo

Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;

Corresponding?author：

SONG Xingbo, Email: songxingbo1981@163.com

Keywords：

Human papilloma virus; Flow fluorescent hybridization technology; Physical examination population

DOI：

10.7507/1002-0179.201907058

Video：

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Objective Using molecular biology method to detect and genotype human papilloma virus (HPV) in women taking physical examination in West China Hospital, Sichuan University, to explore the infection status and genotype distribution of HPV in normal women in Chengdu area, and to provide basis for early effective prevention and control of cervical cancer and domestic research and development of HPV vaccine. Methods Flow fluorescent hybridization technique was used to detect and genotype HPV-DNA in 25 148 healthy women taking physical examination in West China Hospital, Sichuan University between May 1st, 2018 and May 31st, 2019. The overall positive HPV infection rate, HPV genotype distribution, and characteristics of HPV infections were analyzed and calculated, and the HPV infection rates of different age groups were calculated and compared by chi-square test using SPSS 17.0 software. Results The overall positive rate of HPV infection was 12.19% (3 066/25 148). The high-risk HPV genotypes infection rate was 8.69% (2 186/25 148), and the top five subtypes with the highest infection rates were HPV52, HPV53, HPV58, HPV16, and HPV39. The low-risk HPV genotypes infection rate was 4.66% (1 171/25 148), and the top five subtypes with the highest infection rates were HPV61, HPV81, HPV43, HPV44, and HPV6. Single subtype infections were the main infections with a proportion of 81.74% (2 506/3 066), and the most common multiple infections were double infections which accounted for 13.96% (428/3 066). In different age groups, the HPV infection rate of group 60-69 was the highest (12.87%), while that of group 70-89 was the lowest (10.88%), but the difference among different age groups was not statistically significant (χ²=4.035, P=0.544). Conclusion According to the results of this study in women taking physical examination in West China Hospital, Sichuan University, we suggest adding HPV52, HPV53, and HPV58 which have the highest infection rate in high-risk HPV subtypes to the evaluation of domestic HPV vaccine screening and the cervical cancer prevention and control system.

Citation： ZHONG Huiyu, LIU Tangyuheng, WANG Minjin, WU Lijuan, ZHUANG Jie, WANG Jun, SONG Xingbo. Distribution of human papilloma virus subtypes detected by flow fluorescence hybridization in physical examination women in West China Hospital of Sichuan University. West China Medical Journal, 2019, 34(8): 895-899. doi: 10.7507/1002-0179.201907058 Copy

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5.	WHO/ICO Information Centre on HPV and Cervical Cancer. HPV and cervical cancer in the 2007 report. Vaccine, 2007, 25(Suppl 3): C1-C230.
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10.	張建海, 王前, 王巍, 等. 北京市大興區 2117 例女性宮頸人乳頭瘤病毒感染基因亞型狀況分析. 實用預防醫學, 2019, 26(4): 468-470.
11.	王燕, 吳珍珍, 周慶云. 甘肅省婦女宮頸 HPV 感染的流行病學特征. 中國婦幼保健, 2010, 25(36): 5371-5373.
12.	郎妙雙, 馬陳美, 孫麗萍. 宮頸人乳頭瘤病毒感染調查與危險因素分析. 中國預防醫學雜志, 2019, 20(5): 442-445.
13.	吳穎, 曹芬芳, 劉太林, 等. 湖南地區 7076 例女性 HPV 感染情況及分型分析. 實用預防醫學, 2019, 26(4): 474-477.
14.	張美娟, 成祥君, 邱勝豐. 南京地區女性高危型 HPV 亞型分布特征及與宮頸病變的臨床分析. 國際檢驗醫學雜志, 2019, 40(10): 1258-1261.
15.	張亞麗, 彭仁佳, 范毅凱, 等. 鄭州地區 22708 例女性 HPV 感染及基因亞型分布研究. 檢驗醫學與臨床, 2019, 16(11): 1537-1540, 1543.
16.	劉玉萍, 沈太敏, 帥平, 等. 成都市健康體檢婦女人乳頭狀瘤病毒感染及基因亞型分析. 實用醫院臨床雜志, 2015, 12(6): 52-54.
17.	趙玉萍, 王中才, 閆藝, 等. 新疆昌吉地區 1100 例婦女 HPV 感染情況及其亞型分型. 新疆醫學, 2017, 47(12): 1388-1390.
18.	李芳芹, 汪敏, 杜偉平. 719 例女性 HPV 感染情況及其亞型分析. 延安大學學報(醫學科學版), 2017, 15(4): 55-57, 60.
19.	蔡鵬飛, 許澤輝, 王敬仁, 等. 廣西柳州地區 9810 例人乳頭瘤病毒檢測結果分析. 檢驗醫學, 2019, 34(3): 246-248.
20.	鄭紅云, 楊鵬, 申復進, 等. 7746 例婦女宮頸脫落細胞 HPV 基因分型結果研究. 國際檢驗醫學雜志, 2017, 38(24): 3391-3392, 3395.

1. Schiffman M, Clifford G, Buonaguro FM. Classification of weakly carcinogenic human papillomavirus types: addressing the limits of epidemiology at the borderline. Infect Agent Cancer, 2009, 4(1): 8.
2. Torre LA, Bray F, Siegel RL, et al. Global cancer statistics, 2012. CA Cancer J Clin, 2015, 65(2): 87-108.
3. 耿建祥, 黃華藝, 劉建華, 等. HPV 感染疾病相關問題專家共識 (2017). 醫學研究生學報, 2017, 30(12): 1238-1241.
4. Couto E, Saeterdal I, Juvet LK, et al. HPV catch-up vaccination of young women: a systematic review and meta-analysis. BMC Public Health, 2014, 14: 867.
5. WHO/ICO Information Centre on HPV and Cervical Cancer. HPV and cervical cancer in the 2007 report. Vaccine, 2007, 25(Suppl 3): C1-C230.
6. Zeng Z, Yang H, Li Z, et al. Prevalence and genotype distribution of HPV infection in China: analysis of 51,345 HPV genotyping results from China’s largest CAP certified laboratory. J Cancer, 2016, 7(9): 1037-1043.
7. 吳海燕, 肖琳, 周琴, 等. 成都北部地區女性人乳頭瘤病毒感染特征分析. 成都醫學院學報, 2019(3): 393-396, 400.
8. 方莉, 葉紅, 許媛, 等. 四川東北部地區女性高危型人乳頭瘤病毒感染情況分析. 檢驗醫學與臨床, 2017, 14(3): 352-354.
9. 趙佳, 何玉霞, 杜利君. 四川南充地區 3762 例女性高危型人乳頭瘤病毒感染結果分析. 中國計劃生育和婦產科, 2018, 10(8): 94-96.
10. 張建海, 王前, 王巍, 等. 北京市大興區 2117 例女性宮頸人乳頭瘤病毒感染基因亞型狀況分析. 實用預防醫學, 2019, 26(4): 468-470.
11. 王燕, 吳珍珍, 周慶云. 甘肅省婦女宮頸 HPV 感染的流行病學特征. 中國婦幼保健, 2010, 25(36): 5371-5373.
12. 郎妙雙, 馬陳美, 孫麗萍. 宮頸人乳頭瘤病毒感染調查與危險因素分析. 中國預防醫學雜志, 2019, 20(5): 442-445.
13. 吳穎, 曹芬芳, 劉太林, 等. 湖南地區 7076 例女性 HPV 感染情況及分型分析. 實用預防醫學, 2019, 26(4): 474-477.
14. 張美娟, 成祥君, 邱勝豐. 南京地區女性高危型 HPV 亞型分布特征及與宮頸病變的臨床分析. 國際檢驗醫學雜志, 2019, 40(10): 1258-1261.
15. 張亞麗, 彭仁佳, 范毅凱, 等. 鄭州地區 22708 例女性 HPV 感染及基因亞型分布研究. 檢驗醫學與臨床, 2019, 16(11): 1537-1540, 1543.
16. 劉玉萍, 沈太敏, 帥平, 等. 成都市健康體檢婦女人乳頭狀瘤病毒感染及基因亞型分析. 實用醫院臨床雜志, 2015, 12(6): 52-54.
17. 趙玉萍, 王中才, 閆藝, 等. 新疆昌吉地區 1100 例婦女 HPV 感染情況及其亞型分型. 新疆醫學, 2017, 47(12): 1388-1390.
18. 李芳芹, 汪敏, 杜偉平. 719 例女性 HPV 感染情況及其亞型分析. 延安大學學報(醫學科學版), 2017, 15(4): 55-57, 60.
19. 蔡鵬飛, 許澤輝, 王敬仁, 等. 廣西柳州地區 9810 例人乳頭瘤病毒檢測結果分析. 檢驗醫學, 2019, 34(3): 246-248.
20. 鄭紅云, 楊鵬, 申復進, 等. 7746 例婦女宮頸脫落細胞 HPV 基因分型結果研究. 國際檢驗醫學雜志, 2017, 38(24): 3391-3392, 3395.

West China Medical Journal

Distribution of human papilloma virus subtypes detected by flow fluorescence hybridization in physical examination women in West China Hospital of Sichuan University

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