Rat Model of Chronic Obstructive Pulmonary Disease Combined with Type 2 Diabetes Mellitus_West China Medical Journal

Authors：

 GAOLing-yun ¹ ,  RENHui ¹ , YANGXun ² , LIUXiao-jun ² , TAOShao-hua ² , PANLin-hai ²

1. Department of Respiratory Medicine, East Section, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu, Sichuan 610101, P. R. China;
2. Department of Respiratory Medicine, Affiliated Hospital of Chengdu University, Chengdu, Sichuan 610101, P. R. China;

Corresponding?author：

RENHui, Email: 592897298@qq.com

Keywords：

Smoking; Chronic obstructive pulmonary disease; Diabetes mellitus; Rats

DOI：

10.7507/1002-0179.20140610

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To investigate the establishment of rat models with chronic obstructive pulmonary disease (COPD) combined with type 2 diabetes mellitus (DM). Methods Eighty Sprague-Dawley (SD) male rats were randomly divided into four groups:COPD group (n=20), DM group (n=20), COPD combined with DM group (n=20) and normal group (n=20). COPD rats were established by cigarette smoke. Type 2 diabetes rats were modeled by streptozotocin injection. COPD combined with DM rats were modeled by cigarette smoking and streptozotocin injection at the same time. Pathological examination and blood glucose were tested after three months. Results Bronchial epithelium was seriously shedding in COPD+DM group, with alveolar structure damaged and some alveolar fused into bullae. The blood glucose level in COPD+DM group was (27.1±1.1) mmol/L, which was statistically different from other groups (P<0.05). Conclusion Rat model of COPD combined with type 2 DM could be established by cigarette smoking and streptozotocin injection, which can provide an animal model for further medical research.

Citation： GAOLing-yun, RENHui, YANGXun, LIUXiao-jun, TAOShao-hua, PANLin-hai. Rat Model of Chronic Obstructive Pulmonary Disease Combined with Type 2 Diabetes Mellitus. West China Medical Journal, 2014, 29(11): 2012-2015. doi: 10.7507/1002-0179.20140610 Copy

1.	Stinn W, Buettner A, Weiler H, et al. Lung inflammatory effects, tumorigenesis, and emphysema development in a long-term inhalation study with cigarette mainstream smoke in mice[J]. Toxicol Sci, 2013, 131(2):596-611.
2.	Lawal M. Management of diabetes mellitus in clinical practice[J]. Br J Nurs, 2008, 17(17):1106-1113.
3.	高凌云, 楊恂, 官和立, 等. 強化血糖控制對AECOPD合并高血糖患者肺功能的影響[J].國際老年醫學雜志, 2010, 31(5):193-195.
4.	高凌云, 楊恂, 楊建南. 慢性阻塞性肺疾病合并高血糖強化血糖控制的醫療費用特征分析[J]. 重慶醫學, 2012, 41(2):122-123, 126.
5.	Wright JL, Churg A. Cigarette smoke causes physiologic and morphologic changes of emphysema in the guinea pig[J]. Am Rev Respir Dis, 1990, 142(6 Pt 1):1422-1428.
6.	Rakieten N, Rakieten ML, Nadkarni MR. Studies on the diabetogenic action of streptozotocin (NSC-37917)[J]. Cancer Chemother Rep, 1963, 29:91-98.
7.	Kawano K, Hirashima T, Mori S, et al. Spontaneous long-term hyperglycemic rat with diabetic complications. Otsuka Long-Evans Tokushima Fatty (OLETF) strain[J]. Diabetes, 1992, 41(11):1422-1428.
8.	Vestbo J, Hurd SS, Agusti AG, et al. Global strategy for the diagnosis, management, and prevention of chronic obstructive pulmonary disease Gold executive summary[J]. Am J Respir Crit Care Med, 2013, 187(4):347-365.
9.	Schmier JK, Halpern MT, Higashi MK, et al. The quality of Life impact of acute exacerbations of chronic bronchitis (AECB):a literature review[J]. Qual Life Res, 2005, 14(2):329-347.
10.	Rincon M, Irvin CG. Role of IL-6 in asthma and other inflammatory pulmonary diseases[J]. Int J Biol Sci, 2012, 8(9):1281-1290.
11.	Hoidal JR, Niewoehner DE. Cigarette smoke inhalation potentiates elastase-induced emphysema in hamsters[J]. Am Rev Respir Dis, 1983, 127(4):478-481.
12.	Sakai H, Watanabe A, Fujita A, et al. Augmented bronchial smooth muscle contractility induced by aqueous cigarette smoke extract in rats[J]. J Smooth Muscle Res, 2014, 50(0):39-47.
13.	Baker EH, Janaway CH, Philips BJ, et al. Hyperglycaemia is associated with poor outcomes in patients admitted to hospital with acute exacerbations of chronic obstructive pulmonary disease[J]. Thorax, 2006, 61(4):284-289.
14.	Moretti M, Cilione C, Tampieri A, et al. Incidence and causes of non-invasive mechanical ventilation failure after initial success[J]. Thorax, 2000, 55(10):819-825.
15.	Esquivel AL, Pérez-Ramos J, Cisneros J, et al. Effect of obesity and tobacco smoke exposure on inflammatory mediators and matrix metalloproteinases in rat model[J/OL]. Toxicol Mech Methods, 2014:1-38. http://www.ncbi.nlm.nih.gov/pubmed/?term=PMID%3A+25141943.

1. Stinn W, Buettner A, Weiler H, et al. Lung inflammatory effects, tumorigenesis, and emphysema development in a long-term inhalation study with cigarette mainstream smoke in mice[J]. Toxicol Sci, 2013, 131(2):596-611.
2. Lawal M. Management of diabetes mellitus in clinical practice[J]. Br J Nurs, 2008, 17(17):1106-1113.
3. 高凌云, 楊恂, 官和立, 等. 強化血糖控制對AECOPD合并高血糖患者肺功能的影響[J].國際老年醫學雜志, 2010, 31(5):193-195.
4. 高凌云, 楊恂, 楊建南. 慢性阻塞性肺疾病合并高血糖強化血糖控制的醫療費用特征分析[J]. 重慶醫學, 2012, 41(2):122-123, 126.
5. Wright JL, Churg A. Cigarette smoke causes physiologic and morphologic changes of emphysema in the guinea pig[J]. Am Rev Respir Dis, 1990, 142(6 Pt 1):1422-1428.
6. Rakieten N, Rakieten ML, Nadkarni MR. Studies on the diabetogenic action of streptozotocin (NSC-37917)[J]. Cancer Chemother Rep, 1963, 29:91-98.
7. Kawano K, Hirashima T, Mori S, et al. Spontaneous long-term hyperglycemic rat with diabetic complications. Otsuka Long-Evans Tokushima Fatty (OLETF) strain[J]. Diabetes, 1992, 41(11):1422-1428.
8. Vestbo J, Hurd SS, Agusti AG, et al. Global strategy for the diagnosis, management, and prevention of chronic obstructive pulmonary disease Gold executive summary[J]. Am J Respir Crit Care Med, 2013, 187(4):347-365.
9. Schmier JK, Halpern MT, Higashi MK, et al. The quality of Life impact of acute exacerbations of chronic bronchitis (AECB):a literature review[J]. Qual Life Res, 2005, 14(2):329-347.
10. Rincon M, Irvin CG. Role of IL-6 in asthma and other inflammatory pulmonary diseases[J]. Int J Biol Sci, 2012, 8(9):1281-1290.
11. Hoidal JR, Niewoehner DE. Cigarette smoke inhalation potentiates elastase-induced emphysema in hamsters[J]. Am Rev Respir Dis, 1983, 127(4):478-481.
12. Sakai H, Watanabe A, Fujita A, et al. Augmented bronchial smooth muscle contractility induced by aqueous cigarette smoke extract in rats[J]. J Smooth Muscle Res, 2014, 50(0):39-47.
13. Baker EH, Janaway CH, Philips BJ, et al. Hyperglycaemia is associated with poor outcomes in patients admitted to hospital with acute exacerbations of chronic obstructive pulmonary disease[J]. Thorax, 2006, 61(4):284-289.
14. Moretti M, Cilione C, Tampieri A, et al. Incidence and causes of non-invasive mechanical ventilation failure after initial success[J]. Thorax, 2000, 55(10):819-825.
15. Esquivel AL, Pérez-Ramos J, Cisneros J, et al. Effect of obesity and tobacco smoke exposure on inflammatory mediators and matrix metalloproteinases in rat model[J/OL]. Toxicol Mech Methods, 2014:1-38. http://www.ncbi.nlm.nih.gov/pubmed/?term=PMID%3A+25141943.

West China Medical Journal

Rat Model of Chronic Obstructive Pulmonary Disease Combined with Type 2 Diabetes Mellitus

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