Research on the Application of UF-1000i Urine Sediment Analyzer in Rapid Urinary Tract Infection Screening by Receiver Operator Characteristic Curve_West China Medical Journal

Authors：

 MAOZhi-gang , WANGXia , JINYong-mei , XIONGMing , ZHENGQin ,  SUJun

Department of Clinical Laboratory, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;

Corresponding?author：

SUJun, Email: daisy2667@163.com

Keywords：

Urinary tract infection; UF-1000i urinary sediment analyzer; Receiver operator characteristic curve; Bacterial Count; Rapid diagnosis

DOI：

10.7507/1002-0179.20140333

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To investigate the feasibility of quantitative detection of WBC count and bacteria count with UF-1000i urinary sediment analyzer in rapid screening for urinary tract infection by receiver operator characteristic (ROC) curve. Methods From August to December 2013, we used quantitative bacterial culture and UF-1000i automatic urine sediment analyzer respectively to examine asepsis urine specimens of 218 patients with suspected urinary tract infection. Among them, there were 95 males and 123 females, with an average age of 54.7 years old. Results Among the 218 urinary samples, 65 were culture positive specimens. With positive urine culture as the gold standard for making ROC curve, the area under ROC curve for WBC count and bacterial numbers by UF-1000i urine sediment analyzer were respectively 0.839 and 0.894. The cut-off values of Youden index for optimal WBC cell count and bacterial count were ≥31.0/μL and 38.8/μL, respectively. When the above numbers were used as cut-off values, the WBC count sensitivity and specificity were 78.3% and 80.4%, the positive likelihood ratio was 3.99, and the negative likelihood ratio was 1.11. And the bacterial count sensitivity and specificity were 84.3% and 80.6%, the positive likelihood ratio was 4.30, and the negative likelihood ratio was 0.80. Conclusion Using white blood cell count ≥31/μL and bacterial count ≥38.8/μL detected by UF-1000i urine sediment analyzer as the cut off values of noninvasive screening indexes has a very important value in screening for urinary tract infection in the early stage, determining whether there is a need for urine culture, and guiding clinical rational application of antibiotics

Citation： MAOZhi-gang, WANGXia, JINYong-mei, XIONGMing, ZHENGQin, SUJun. Research on the Application of UF-1000i Urine Sediment Analyzer in Rapid Urinary Tract Infection Screening by Receiver Operator Characteristic Curve. West China Medical Journal, 2014, 29(6): 1092-1095. doi: 10.7507/1002-0179.20140333 Copy

1.	Graham JC, Galloway A. ACP Best Practice No 167: the laboratory diagnosis of urinary tract infection[J]. J Clin Pathol, 2001, 54(12): 913-919.
2.	杭建峰, 孫朝暉, 石玉玲, 等. Sysmex UF-1000i全自動尿沉渣分析儀的性能分析及臨床應用[J]. 檢驗醫學, 2011, 26(2): 108-110.
3.	黃春光, 楊潔飛, 李小平. UF-1000i尿沉渣分析系統在尿路感染快速診斷中的應用[J]. 實驗與檢驗醫學, 2009, 27(5): 489-490.
4.	叢玉隆. 尿液沉渣檢查標準化的建議[J]. 中華檢驗醫學雜志, 2002, 25(4): 56-57.
5.	陳衛中, 倪宗瓚, 潘曉平, 等. 用ROC曲線確定最佳臨界點和可疑值范圍[J]. 現代預防醫學, 2005, 32(7): 729-731.
6.	石晶, 趙敏, 歐陽金銘. 以UF-100尿分析儀的WBC和細菌定量結果建立閾值篩選尿細菌培養標本[J]. 臨床檢驗雜志, 2008, 26(3): 181-182.
7.	Manoni F, Fornasiero L, Ercolin M, et al. Cutoff values for bacteria and leukocytes for urine flow cytometer Sysmex UF-1000i in urinary tract infections[J]. Diagn Microbiol Infect Dis, 2009, 65(2): 103-107.
8.	Delanghe JR, Kouri TT, Huber AR, et al. The role of automated urine particle flow cytometry in clinical practice[J]. Clin Chim Acta, 2000, 301(1-2): 1-18.
9.	吳晶晶, 劉佳, 梅亞寧, 等. 尿路感染病原菌分布及耐藥性分析[J]. 南京醫科大學學報·自然科學版, 2010, 30(9): 1356-1359.
10.	陳麗, 張坤, 李月強, 等. Uf-1000i尿沉渣分析儀檢測細菌的性能及對尿路感染的篩查價值[J]. 華中科技大學學報·醫學版, 2011, 40(3): 354-356, 360.
11.	王盛華, 劉蕓, 肖平, 等. 健康人群Sysmex UF-1000i尿液流式分析參考范圍的調查[J]. 國際檢驗醫學雜志, 2012, 33(4): 442-443, 455.
12.	鄧益斌, 張梁, 鄧巧瑩, 等. 尿沉渣細菌定量計數與尿液細菌培養比較及其在篩查泌尿系統感染中的臨床應用[J]. 中國實驗診斷學, 2010, 14(3): 449-450.
13.	羅劍, 孫朝暉, 甘燕玲, 等. UF-1000i尿沉渣分析儀對尿路感染早期診斷的價值[J]. 華南國防醫學雜志, 2010, 24(1): 61-62.
14.	吳華軍, 呂青山, 侯史文, 等. UF-1000i尿液分析儀在尿路感染診斷中的應用[J]. 浙江實用醫學, 2010, 15(4): 324-326.

1. Graham JC, Galloway A. ACP Best Practice No 167: the laboratory diagnosis of urinary tract infection[J]. J Clin Pathol, 2001, 54(12): 913-919.
2. 杭建峰, 孫朝暉, 石玉玲, 等. Sysmex UF-1000i全自動尿沉渣分析儀的性能分析及臨床應用[J]. 檢驗醫學, 2011, 26(2): 108-110.
3. 黃春光, 楊潔飛, 李小平. UF-1000i尿沉渣分析系統在尿路感染快速診斷中的應用[J]. 實驗與檢驗醫學, 2009, 27(5): 489-490.
4. 叢玉隆. 尿液沉渣檢查標準化的建議[J]. 中華檢驗醫學雜志, 2002, 25(4): 56-57.
5. 陳衛中, 倪宗瓚, 潘曉平, 等. 用ROC曲線確定最佳臨界點和可疑值范圍[J]. 現代預防醫學, 2005, 32(7): 729-731.
6. 石晶, 趙敏, 歐陽金銘. 以UF-100尿分析儀的WBC和細菌定量結果建立閾值篩選尿細菌培養標本[J]. 臨床檢驗雜志, 2008, 26(3): 181-182.
7. Manoni F, Fornasiero L, Ercolin M, et al. Cutoff values for bacteria and leukocytes for urine flow cytometer Sysmex UF-1000i in urinary tract infections[J]. Diagn Microbiol Infect Dis, 2009, 65(2): 103-107.
8. Delanghe JR, Kouri TT, Huber AR, et al. The role of automated urine particle flow cytometry in clinical practice[J]. Clin Chim Acta, 2000, 301(1-2): 1-18.
9. 吳晶晶, 劉佳, 梅亞寧, 等. 尿路感染病原菌分布及耐藥性分析[J]. 南京醫科大學學報·自然科學版, 2010, 30(9): 1356-1359.
10. 陳麗, 張坤, 李月強, 等. Uf-1000i尿沉渣分析儀檢測細菌的性能及對尿路感染的篩查價值[J]. 華中科技大學學報·醫學版, 2011, 40(3): 354-356, 360.
11. 王盛華, 劉蕓, 肖平, 等. 健康人群Sysmex UF-1000i尿液流式分析參考范圍的調查[J]. 國際檢驗醫學雜志, 2012, 33(4): 442-443, 455.
12. 鄧益斌, 張梁, 鄧巧瑩, 等. 尿沉渣細菌定量計數與尿液細菌培養比較及其在篩查泌尿系統感染中的臨床應用[J]. 中國實驗診斷學, 2010, 14(3): 449-450.
13. 羅劍, 孫朝暉, 甘燕玲, 等. UF-1000i尿沉渣分析儀對尿路感染早期診斷的價值[J]. 華南國防醫學雜志, 2010, 24(1): 61-62.
14. 吳華軍, 呂青山, 侯史文, 等. UF-1000i尿液分析儀在尿路感染診斷中的應用[J]. 浙江實用醫學, 2010, 15(4): 324-326.

West China Medical Journal

Research on the Application of UF-1000i Urine Sediment Analyzer in Rapid Urinary Tract Infection Screening by Receiver Operator Characteristic Curve

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