PURPOSE:To observe the clinical features of the macular hemorrhage in myopes. METHOD:Twenty-four patients(30 eyes)with myopic macular hemorrhage were examined with slitlamp biomicroscopy,funduscope,A/B ultrasonography,and fundus fluorecein angiography(FFA). The patients were followed up for 3~18 months(average 12 months). RESULTS: Four of 26 eyes with macular hemorrhage examined with FFA were found to be due to choroidal neovaseulature,and they were associated with posterior staphyloma. The other 22 eyes without neovascular change were thought to be simple type,and 19 of them were associated with lacquer cracks. The hemorrhage in simple type cases deminished usually within 1~3 months. CONCLUSION:Myopic macular hemorrhagic eyes of neovascular type resulted usually in recurrent hemorrhage and worse prognosis in visual acuity than those of simple type. （Chin J Ocul Fundus Dis,1996,12: 220-222）
目的:探討宮頸癌體外放射治療加后裝腔內治療的療效和放射反應。方法:對76例宮頸癌患者均采用體外放射治療加60Co 后裝腔內治療臨床資料進行回顧性分析。結果:全組 5年總生存率為 63.7%,Ⅰ期生存率為88.9%,Ⅱ期為 71.9%,Ⅲ期為48.1%,Ⅳ期為 20.0%。10年總生存率為 31.6%,Ⅰ期生存率為 53.3%,Ⅱ期為33.3%,Ⅲ期為 22.0%,Ⅳ期為 0.0%,經Log Rank法檢驗各期生存率有顯著差異(Plt;0.01)。放射治療后發生陰道粘連 9例(11.8%),放射性膀胱炎 6例(7.9%),放射性直腸炎13例(17.1%)。放射性直腸炎按 RTOG 晚期放射損傷分級方案分級,1級7例(53.8%),2級4例(30.8%),3級 2例(15.4%),4級 0例(0.0%)。結論:體外放射治療加60Co后裝腔內治療宮頸癌療效肯定,副作用少。但如何進一步提高生存率,盡可能減少放射反應仍是今后研究的方向。
Abstract: Objective To investigate the feasibility of the bone marrow mesenchymal stem cells (BMSCs) as the seed cells for construction of small diameter blood vessels and its induced mechanisms. Methods The bone marrow cells were obtained from hind femur and tibia of male Sprague-Dawley（SD） rats with a body weight of 100 g. The cells were purified by whole bone marrow primary culture before repeated passage in vitro amplification. Cell morphology was observed, and expressions of CD34, CD90, and CD105 cell factors were examined by flow cytometry to identify whether they were the BMSCs. Then, the BMSCs obtained were divided into the experiment group and the control group. The cells in the experiment group were induced to differentiate into the vascular smooth musclelike cells by the Dulbecco’s modified Eagle’s mediumlow glucose(DMEM-LG) plus alltrans retinoic acid and dbcAMP, while the cells in the control group were cultured by the normal DMEM-LG. We observed the morphological characteristics of the BMSCs and detected the expressions of smooth muscle-α actin (SM-α-actin), calponin, and vascular smooth muscle myosin heavy chain(SMMHC) by immunofluorescence and flow cytometry with the fifth generations cells after induction. Results The cells obtained through primary culture appeared spindleshaped and showed characteristic swirling growth. The surface marker CD34 was negative, while CD90 and CD105 were positive. After induction, the cells in the experiment group grew slowly and were slightly ovalshaped. The expression of SM-α-actin, calponin, and SMMHC was significant in the experiment group. In the control group, cell morphology and cell growth were similar to the those of BMSCs in the experiment group, but the expression of SM-α-actin, calponin, and SMMHC was negative. Conclusion The BMSCs can be induced to differentiate into the phenotype of vascular smooth musclelike cells by alltrans retinoic acid，the induced cells which can act as seed cells for tissue engineering construction of small diameter blood vessels.
Objective To study the retinal light sensitivity of central visual field in normal children. Methods The QZS-Ⅱ automated perimetry was used to assess the visual field of centro-30deg;and centro-6deg; in normal or ametropic eyes in 60 eyes of 5～9 years old children. Results The mean sensitivity(MS)was not influenced by sex,age and laterality and ametropia of the eye.The normal type of dB distribution was obviously higher than the abnormal(P＜0.01).We set normal range as 30deg;MS＞19.3 dB, 6deg;MS＞22.5 dB.The abnormality of value or distribution didnprime;t appear in the same field. Conclusions In normal children,the dB distribution of visual field was mainly of the normal type.We suggest that in evaluating function of visual filed of the children,the dB distribution of centro-30deg;and centro-6deg;field and the value of MS should be included. (Chin J Ocul Fundus Dis, 1999, 15: 137-138)
目的:觀察七氟醚靶控用于低流量吸入麻醉維持的臨床規律及血流動力學變化,尋求靶控下的藥物量化指標。方法:選擇60例20～60歲手術患者,隨機分2組每組30例:P組(丙泊酚)常規實施全憑靜脈TCI靶控麻醉;S組(七氟醚),實施低流量七氟醚靶控吸入麻醉(BIS值40)。分別記錄誘導前(T1),插管后5(T2)、15(T3)、30(T4)45(T5)、60(T6)、90(T7)、120(T8)min時的、MBP、HR、;Sev組患者加記各個時點的七氟醚IT、ET的MAC值。結果:T2點兩組均比T1 降低(Plt;0.05),S 組高于P組(Plt;0.05),兩組比較T3至T7無顯著性差異(Pgt;0.05);S 組T3 至T7 各點ET值無統計學差異,ET%:2.46,約1.4 MAC。結論:(1)七氟醚誘導較靜脈麻醉誘導患者血流動力學穩定,(2)低流量七氟醚靶控吸入麻醉維持平穩,調控簡便,效果良好。
Objective To investigate the clinical features, etiological classification and staging of epiretinal macular membrane(MEM)． Methods Clinical materials of 194 cases of MEM diagnosed by fundus fluorescein angiography in outpatient department of eye clinic in this hospital from 1983 to 2000 were retrospectively analyzed． Results There were typical clinical symptoms and signs of MEM in all of this 222 eyes of 194 patients． Etiological classification revealed that 4 cases were congenital(2．12%), 22 cases were secondary(11．34%), and 168 cases were idio pathic(86．60%)． Staging of course of disease indicated that 119 eyes were in early stage(53．60%), 72 eyes were in middle stage(32．43%), and 31 eyes were in late stage(13．96%)． Conclusion MEM may be classified as congenital, secondary and idiopathic type according to its pathogenesis , as early, middle and late stage according to the clinical course of disease．This can be helpful in treating the disease． (Chin J Ocul Fundus Dis, 2001,17:210-213)
Objective To explore the expression and activation of transcription factor E2F1 in cultured human retinal pigment epithelium (RPE) cells. Methods Cultured human RPE cells were divided into two groups after synchronization: one was cultured in Dulbecco′s modified Eagle′s medium (DMEM) without serum; the other was cultured in DMEM supplemented with 20% serum of newborn calf. The expressions of E2F1 protein in two groups were detected by Western blot analysis. The E2F1-DNA binding activities were measured by gel mobility-shift assay(EMSA). Results E2F1 protein of 60 000 molecular weight was detected in the nuclear extract of human RPE cells, and serum stimulation could increase its expression（P＜0.001）. EMSA exhibited the increased binding activity of E2F1 in the serum-stimulated RPE cells with DNA. Conclusions E2F1 is expressed in the nuclei of human RPE cells. Serum stimulation can increase its protein expression as well as binding activity, so as to play a regulation role of gene transcription. (Chin J Ocul Fundus Dis, 2002, 18: 224-226)
Objective To observe the clinical features of polypoidal choroidal vasculopathy (PCV) in Chinese patients.Methods Nine cases (9 eyes ) were examined with fundus examination, fundus fluorescein angiography (FFA) and indocyanine green angiography angiography (ICGA).Results FFA and ICGA showed the branching vascular networks (7 cases) and polyplike dilation at terminals of branches (9 cases), which mainly located in macular area (8 cases) and in peripapillary area (1 case), and which accompanied hemorrhagic or serous pigment epithelial detachment in 7 cases,and 4 of 7 cases had a significant horizontal black-white damarcation line. It definitely differed from fine choroidal neovascularization (CNV).Conclusion PCV in Chinese patients has the cardinal clinical features, i.e., polyplike lesions located mainly in macular area and most cases accompanied by hemorrhagic or serous pigment epithelium detachment. (Chin J Ocul Fundus Dis，2003，19：269-332)
目的:探討行氣管切開術搶救成功的重型顱腦損傷及高血壓腦出血患者直接除管的安全性和可行性。方法:在507監護儀行SPO2監測和嚴密觀察下,不經過試阻管而直接將氣管套管拔除。結果:本組除1例患者因帶管時間長,切口周圍氣管內炎性肉芽生長而重新插管外,其余患者呼吸平穩,呼吸道通暢,無呼吸急促、嗆咳、紫紺及SPO2降低。結論:此法避免了傳統除管前試阻管的繁鎖和由阻管而引起的多種不良反應,有臨床實用價值。