Due to the complexity of etiology and a lot of complication of diabetes mllitus, the reliable conclusion of studies of etiology and treatment of diabtets mellitus should rely on randomized controlled trial and systematic review. Alongside the development of meta-analysis, systematic reviewes have provided many beneficial information, including gene mutation and diabetes mellitus, evaluation of risk factors, diagnostic test and treatment of diabets mellitus.
ObjectiveTo detect the induction of inducible nitric oxide synthase (iNOS) and nitric oxide (NO) production in immunostimulated retinal pigment epithelial (RPE) cells to seek for the supplying of the arginine, a substrate for NOS; as well as the effects of produced NO on the tight junction of RPE-J cells. MethodsRat′s RPE-J cells were treated with interferon-γ(INF-γ), tumor necrosis factor-α(TNF-α) and lipopolysaccharide (LPS), and Northern and Western blotting were applied to analyze the expression of the citrulline-NO cycle enzymes and related enzymes and the effect of dexamethasone and cyclic adenosine monophosphate (camp) on the expression of iNOS. Immunocytochemistry reaction and Western blotting were used to evaluate the effect of produced NO on the tight junctions of RPE-J cells.ResultsiNOS and argininosuccinate synthetase (AS) were highly induced at both mRNA and protection levels in immunostimulated RPE cells while arginiosuccinate lyase (AL) was not induced. NO was produced by cells after stimulation with TNFα, IFNγ and LPS. The induction of iNOS mRNA and the production of NO by these immunostimulated cells was further enhanced by cAMP. NO was produced from citrulline as well as from arginine. And the produced NO impaired the tight junction of RPE-J cells, decreased the production of tight junction related protein ZO-1.ConclusionIn activated RPE-J cells, citrullinearginine recycling is important for NO production, and the produced NO weakened the function of tight junction of RPE-J cells.(Chin J Ocul Fundus Dis, 2005,21:32-36)
【摘要】 目的 優化牙釉質電子順磁共振（EPR）譜擬合程序。 方法 將牙釉質EPR譜擬合程序中的RIS-BGS偏移量設置為定參，取優化值；增加手動擬合功能，可以將參數初始值直接代入高斯模型得到擬合譜。 結果 將牙釉質EPR譜擬合程序中RIS-BGS偏移量設置為定參后，其優化值為0.529，減少了變參數量；采用手動擬合功能有助于調整參數初始值；在自動擬合程序對于個別樣品譜不能給出正確結果情況時，仍然可以通過手動擬合來得到一個近似的擬合結果。 結論 優化了EPR譜擬合程序的應用過程。【Abstract】 Objective To optimize the enamel electron paramagnetic resonance (EPR) spectrum fitting procedure. Methods RIS-BGS offset was set as a determined parameter in enamel EPR spectrum fitting program, and an optimized value was given for RIS-BGS offset. A manual fitting function was designed that can substite the initutial value into the Gaussian model function directly. Results RIS-BGS offset was set as a determined parameter, the optimized value of which was 0.529. The number of parameters decreased; the manual fitting function maked it easier to select initial values for parameters. When the auto fitting program failed to give a correct result for some EPR spectrum, a fitting result could still be obtained with the manual fitting method. Conclusion The application of the spectrum fitting procedure can be optimized.