Objective To investigate the iron regulated locus in Klebsiella pneumoniae isolates from blood culture of liver abscess patients in Sichuan Provincial People’s Hospital. Methods From January to December of 2015, a total of 10 isolates of Klebsiella pneumoniae were collected from blood culture of liver abscess patients from Sichuan Provincial People’s Hospital. The genomic DNA was extracted to identify the genes of iroB, iroC, and iroD by PCR, and data was further analyzed by Graphpad Prism 5 software. Results Among the 10 Klebsiella pneumoniae clinical strains, 9 strains were iroB positive strains, 9 strains were iroC positive strains, and 10 strains were iroD positive strains, 9 strains were iroB/C/D triple positive. Conclusion The current study suggests that the frequency of triple positive of iroB/C/D in Klebsiella pneumoniae is high in isolates from liver abscess patients, the triple positive of iroB/C/D may contribute to liver abscess.
Objective To investigate the expressions of β1, 3-N-acetyl glucosaminyl transfrases ( Fringe) ( RFNG, LFNG and MFNG) in lung tissues and lung T cells isolated from asthmatic mice, and to explore the role of Fringe in pathogenesis of asthma. Methods Asthmatic BALB/ c mouse model was established by inhalation of ovalbumin after intraperitoneal injection. Meanwhile, the control groups were established by normal saline. Lung tissues were sampled after 24 hours since the last stimulation. T cells were isolated from the lung tissues using percol and NylonFiber. The mRNA expressions of three kinds of Fringe in the lung tissues and lung T cells were examined by reverse transcription-PCR ( RT-PCR) . The protein expressions of Fringe in the lung tissues were detected by Western blot. Results The mRNA expressions of RFNG, LFNG and MFNG were detectable in the lung tissues and lung T cells. The mRNA expressions of RFNG increased in the asthmatic group compared with the control group( lung tissues: 0. 92 ±0. 35 vs 0. 51 ±0. 13, P lt; 0. 01; lung T cells: 0. 33 ±0. 06 vs 0. 18 ±0. 07, P lt; 0. 01) . LFNG mRNA had lower expression level in the asthmatic group( lung tissue: 0. 77 ±0. 32 vs 1. 61 ±0. 31, P lt; 0. 01; lung T cells: 0. 49 ±0. 19 vs 0. 71 ±0. 03, P lt;0. 01) . No difference on the mRNA expression of MFNG was found in the lung tissues( 1. 44 ±0. 29 vs 1. 70 ±0. 44, P gt; 0. 05) . MFNG mRNA expression decreased in the asthmatic group compared with the control group in the T cells( 1. 17 ±0. 04 vs 0. 68 ±0. 07, P lt;0. 05) . The results of western blot were consistent with RT-PCR results of the lung tissues. The expressions of RFNG increased in the asthmatic group( 1. 17 ±0. 04 vs 0. 68 ±0. 07, P lt;0. 05) . The expression of MFNG has no difference between two groups( 8. 10 ±0. 60 vs 9. 12 ±0. 07, P gt;0. 05) . LFNG had a lower expression in the asthmatic group( 4. 11 ±0. 38 vs 6. 41 ±0. 11, P lt; 0. 05) . Conclusion The abnormal expressions of three kinds of Fringe may be involved in the pathogenesis of asthma.
【摘要】 目的 探討男性和女性糖尿病腎病患者危險因素之間的差異。方法 收集2004年1月—2008年12月以糖尿病腎病為診斷的住院患者1 300例,根據入院日期以系統的方法隨機納入650例患者,排除20例尿常規正常和血肌酐值正常的患者,共630例,其中男342例,女288例,平均年齡65歲。288例女性患者中有271例為絕經后婦女,平均年齡48歲。比較女性和男性糖尿病腎病患者危險因素的差別。結果 ①絕經后糖尿病腎病患者占女性患者的94.1%；②女性糖尿病腎病患者組收縮壓、脈壓及糖化血紅蛋白水平高于男性糖尿病腎病患者組,兩者有統計學意義(Plt;0.05)；③兩組患者在發病年齡,病程,糖尿病家族史,空腹血糖水平,低密度脂蛋白,高密度脂蛋白,血肌酐,24 h尿蛋白定量、是否使用血管緊張素轉換酶抑制劑,血管緊張素受體拮抗劑,胰島素,是否透析,死亡率均無統計學意義(Pgt;0.05)。結論 在女性糖尿病腎病患者中絕經后婦女所占比例較高,女性糖尿病腎病患者收縮壓、脈壓及糖化血紅蛋白水平均高于男性糖尿病腎病患者,雌激素的水平可能導致差異的產生。
ObjectiveTo summarize strategy of diagnosis and treatment of thrombotic iliac vein stenosis in recent years.MethodThe relevant literatures in recent 5 years on the current status and trend of diagnosis and treatment of the thrombotic iliac vein stenosis were reviewed.ResultsAt present, the diagnostic methods of thrombotic iliac vein stenosis mainly included that the multi-slice spiral CT, venography, intracavitary ultrasound, magnetic resonance imaging, color Doppler ultrasound and other imaging examinations, each of which had its advantages and disadvantages. There were still no uniform criteria whether a filter should be placed, whether the stents should and how to be placed, and when to intervene in the patients with acute thrombotic iliac vein stenosis. There was no definite conclusion when and how to effectively intervene in the patients with chronic thrombotic iliac vein stenosis.ConclusionsIncidence of thrombotic iliac vein stenosis is high and sequela is severe. Early diagnosis and reasonable treatment are very important. Only strictly grasping indications, combining medical technology and patient’s condition, and adopting appropriate treatment strategy, can make patients’ maximal benefit.
Objective To investigate the value of thyroid stimulating hormone (TSH) inhibition therapy in postoperative patients with differentiated thyroid carcinoma. Methods One hundred and six patients with differentiated thyroid carcinoma after total or near-total thyroidectomy were divided into two groups: TSH inhibition therapy group and thyroid hormone replacement therapy group. The non-recurrence and (or) non-metastasis rate in two groups was analyzed retrospectively by Wilcoxon statistical analysis. Results The non-recurrence and (or) non-metastasis rate of 3-， 5- and 10-year in TSH inhibition therapy group were 98.31%， 92.41% and 75.45% respectively， and were higher than those (93.57%， 84.18% and 52.06% respectively) in thyroid hormone replacement therapy group （P=0.046 5）. Conclusion TSH inhibition therapy after total or near-total thyroidectomy may be an essential treatment for differentiated thyroid carcinoma patients.
ObjectiveTo systematically review the influence of childhood psychosocial stress on pubertal emotional and behavioral problems. MethodsThe PubMed, OVID, EBSCO, Web of Science, CBM, VIP, WanFang Data and CNKI databases were electronically searched to collect studies on the relationships between childhood psychosocial stress and pubertal emotional and behavioral problems from inception to February 29, 2024. Two reviewers independently screened the literature, extracted data and assessed the risk of bias in the included studies. Qualitative methods were then used to analyze the data. ResultsA total of 41 cohort studies were included. The outcomes of 19 studies involved pubertal emotional problems, 26 studies involved behavioral problems, and 7 studies involved overall problems. The results showed that depression (14/19) and anxiety (8/19) were the most commonly reported emotional behaviors. Most studies (17/19) showed that childhood psychological stress had a positive predictive effect on pubertal emotional problems. Behavioral problems involved many outcomes, including smoking, drinking, illegal substance use, self-injurious behavior, suicide, externalizing behavior, criminal behavior, bullying behavior, sexual behavior, mobile phone dependence, etc. However, few studies were on the same behaviors, and the relationship between childhood psychosocial stress and behavioral problems was unclear. ConclusionChildhood psychosocial stress may have a positive predictive effect on depression and anxiety. The associations between childhood psychosocial stress and pubertal behavioral problems and other emotional problems remain to be determined by more studies.
Objective To compare and research the process of woundhealing in occlusive moist environment and dry environment on the skin donor site. Methods The wound healing of adult skin donor site was studied by clinical observation, histological and electromicroscopical examinations on the operative day and the 1st, 2nd, 3rd, 4th, 7th days postoperatively, each skin donor site was divided into two parts: occlusive environment and dry environment. Results The wounds of occlusive moist environment healed faster than those of dry environment; thefibroblasts were more active and activated earlier, revascularization and re-epithelialization happened earlier and more quickly. Conclusion In occlusive environment, more active fibroblasts can accelerate granulation growth; quicker regenerative capillaries bring more nourishment; quicker re-epithelialization accelerates the wound healing.
ObjectiveTo investigate the mental state of people during the outbreak of coronavirus disease 2019.MethodsA survey was conducted on 2 141 subjects using the anxiety and terror subscales of the 90-item symptom self-rating scale and a self-designed questionnaire by internet.ResultsThe anxiety level of the subjects in epidemic situation were slightly worse than the national average level in the normal period, and the terror level of the subjects in epidemic situation were extremely worse than the national average level in the normal period (P<0.05). The mental state of the subjects showed significant differences in gender, age, source and Wuhan contact history. There was a significant positive correlation between epidemic information and anxiety, fear and sleep status of subjects. The anxiety and fear levels of subjects with the cognitive tendency of systems thinking were significantly lower than other subjects (P<0.05).ConclusionsStress responses have emerged in the population during the outbreak of coronavirus disease 2019, mainly caused by the epidemic events directly threatened the health and safety of life. People need timely mental support provided by public emergency management.
目的 研究C-C亞族趨化因子單核細胞趨化蛋白-4（MCP-4/CCL13）在系統性紅斑狼瘡（SLE）患者外周血的表達水平，并分析MCP-4的水平與狼瘡腎炎的關系，以探討MCP-4在SLE發病機制中可能起的作用。 方法 選取2007年9月－2010年8月在四川大學華西醫院和宜賓市第一人民醫院診斷明確的SLE及類風濕關節炎（RA）患者各40例。另收入正常健康對照組（20例），應用酶聯免疫吸附試驗定量方法測定SLE組、RA患者和正常健康對照者血清中MCP-4的水平，SLE患者根據有無腎臟受累分為非狼瘡腎炎組和狼瘡腎炎組，其中非狼瘡腎炎組20例，狼瘡腎炎組20例，并分析SLE組血清MCP-4水平是否與抗核抗體、補體C3、C4等指標及SLE疾病活動指數SLEDAI評分相關性，血清MCP-4水平采用方差分析、LSD-t檢驗和Spearman相關進行統計分析。 結果 血清MCP-4水平SLE組為（216.32 ± 12.65）pg/mL，RA組為（203.79 ± 18.64）pg/mL，正常健康對照組為（125.13 ± 11.08）pg/mL。SLE組、RA組血清MCP-4水平與正常健康對照組相比均有統計學意義（P＜0.05），SLE組與RA組比較血清MCP-4水平無統計學意義（P＞0.05）；SLE患者中狼瘡腎炎組與非狼瘡腎炎組比較血清MCP-4水平無統計學意義（P＞0.05）。SLE組血清MCP-4水平與抗核抗體、補體C3、C4等指標及SLEDAI評分無相關性。 結論 MCP-4在SLE組患者血清中表達增高，MCP-4可能參與了SLE的發病過程，可能成為SLE新的血清學有用指標并作為治療的靶點。
Objective To review the research progress of the skin flap, fascial flap, muscle flap, and myocutaneous flap for repairing soft tissue defects around the knee so as to provide information for clinical application. Methods Domestic and abroad literature concerning the methods of soft tissue repair around the knee in recent years was reviewed extensively and analyzed. Results Fascial flaps meet the requirements of thin, pliable, and tough skin in the soft tissue repair around the knee. Myocutaneous flaps and muscle flaps have more abundant blood supply and anti-infection function. Free skin flaps are the only option when defects are extensive and local flaps are unavailable. Conclusion Suitable flaps should be chosen for soft tissue repair around the knee according to defect position, depth, and extent. Fascial flaps may be selected as the first flaps for defects repair because of excellent aesthetic results and less injury at the donor site.