Objective To study the molecular characteristics of RNA binding protein aldehyde dehydrogenase 18 family member A1 (ALDH18A1) in esophageal carcinoma cells (KYSE150 cells) and its effect on tumor growth. MethodsHuman esophageal squamous cell (KYSE150 cells) was cultured in vitro. At the same time, RNA co-immuno precipitation technology was used to study the binding of RNA and protein in the cell, and the corresponding RNA-protein complex was precipitated by the antibody of the target protein to separate and purify the captured RNA. The molecular characteristics of ALDH18A1 binding RNA were analyzed, and KyotoEncyclopedia of Genes and Genomes cluster analysis was performed for ALDH18A1 binding target genes. Results Protein immunoblotting experiments showed that the target protein was well enriched by antibodies. ALDH18A1 had extensive RNA binding activity, with significant enrichment in regions such as coding sequences, intron, and 5’untranslated region. ALDH18A1 mainly bound to the UGUAAUC motif of RNA. The cluster analysis showed that the RNA molecules bound to ALDH18A1 mainly participated in focal adhesion, central carbon metabolism in cancer, cell cycle, spliceosome, RNA transport, and ubiquitin mediated protein hydrolysis. Conclusion ALDH18A1 has the function of binding to RNA molecules and may play a role in the expression of esophageal cancer-related genes and related biological processes.
Objective To investigate the clinical and pathological characteristics, prognosis and treatment strategies of adenocarcinoma in situ (AIS) and minimally invasive adenocarcinoma (MIA). Methods We retrospectively analyzed the clinical data of 489 patients with AIS and MIA in our hospital from January 2007 to August 2015. There were 122 males and 367 females with an average age of 26–78 (51±9) years. According to the pathological types, they were divided into the AIS group (246 patients) and the MIA group (243 patients). In the AIS group, there were 60 males and 186 females with an average age of 50±7 years. In the MIA group, there were 62 males and 181 females with an average age of 54±5 years. The clinicopathological features, surgical methods and prognosis of the two groups were compared. Results There were significant differences in age, value of carcino-embryonic antigen (CEA), nodule shape and nodule size between the AIS and MIA groups (P<0.05). AIS patients were mostly under the age of 60 years with the value of CEA in the normal range which often appeared as pure ground-glass opacity lung nodules <1 cm in diameter on the CT scan. MIA often appeared as mixed ground-glass nodules <1.5 cm in diameter, accompanied by bronchiectasis and pleural indentation. The 5-year disease-free survival rate of the AIS and MIA groups reached 100%, and there was no statistical difference in the prognosis between the two groups after subtotal lobectomy (pulmonary resection and wedge resection) and lobectomy, systematic lymph node dissection and mediastinal lymph node sampling. Conclusion The analysis of preoperative clinical and imaging features can predict the AIS and MIA and provide individualized surgery and postoperative treatment program.
Objective To observe the effects of valsartan/ hydrochlorothiazide and valsartan on left ventricular thickness and the left ventricular diastolic function in patients with essential hypertension and left ventricular hypertrophy and impaired left ventricular diastolic function. Methods 56 patients of essential hypertension with left ventricular hypertrophy and impaired left ventricular diastolic function were randomized into two randomized double-blind groups, valsartan/hydrochlorothiazide (HCTZ) 80/12.5 mg o.d were gave to A group and valsartan 80 mg o.d were gave to B group. The dosage would be doubled in patients whose SDBP ≥ 12 kPa or SSBP ≥ 18.7 kPa after 4 weeks. Treatment lasted for 6 months. Result At the end of 6 months, valsartan/ hydrochlorothiazide and valsartan significantly reduced BP from baseline (Plt;0.01), there was significant difference in reducing BP between the two groups (Plt;0.05). Indexes of left ventricular diastolic function (IVST, LVPWT, LVMI) significantly decreased (Plt;0.01). LVEF increased significantly (Plt;0.01). There was significant difference in IVST, LVPWT, LVMI and LVEF between two groups (Plt;0.05). Conclusion Valsartan/ hydrochlorothiazide (HCTZ) can not only decrease blood pressure effectively, but also can significantly improve left ventricular hypertrophy and left ventricular diastolic function.
目的 討論多窗技術+凝血酶封閉在CT導向下經皮肺穿刺活檢中的應用價值。 方法 2009年6月－2010年3月收集分析由同一工作組連續完成的CT導向下肺穿刺活檢患者共128例，其中A組58例，采用雙窗技術+注射生理鹽水；B組70例，采用多窗技術+注射凝血酶）。比較兩組患者的診斷陽性率、氣胸及肺出血發生率的差異。 結果 128例均穿刺成功，A組的穿刺診斷陽性率、氣胸發生率及肺出血發生率分別為87.9%、13.8%、17.5%。B組的穿刺診斷陽性率為92.9%，氣胸發生率為8.6%，肺出血發生率為5.7%。兩組穿刺診斷陽性率和氣胸發生率的差異無統計學意義（P＞0.05）；B組肺出血的發生率均明顯低于A組，兩組間肺內出血的發生率差異有統計學意義（P＜0.05）。 結論 多窗技術+凝血酶針道封閉技術能有助于減少氣胸、肺出血等肺穿刺活術的并發癥，具有重要的臨床應用價值。