Objective To explore the microRNA （miRNA） expression changes and related miRNA characteristics of colorectal cancer （CRC） with hepatic metastasis by miRNA microarray. Methods The fresh specimens of primary CRC were collected in 10 patients during operation， which with hepatic metastasis or not. miRNA microarray analysis was performed to compare the miRNA expression levels in two groups. The different expression levels of miRNA were validated by quantitative real-time PCR analysis. Results A total of six dysregulated miRNAs were identified in the CRC patients with hepatic metastasis comparing with CRC patients without hepatic metastasis， including 3 up-regulated miRNAs （miR-224， miR-1236， and miR-622） and 3 down-regulated miRNAs （miR-155， miR-342-5p， and miR-363）， and the quantitative real-time PCR result of miR-224 consisted with the microarray finding. Conclusions miR-224 may be involved in the process of CRC with hepatic metastasis pathogenesis. miR-224 would be a research direction on a new biomarker or therapic method in CRC with hepatic metastasis.
目的:探討開顱夾閉和血管內栓塞治療顱內動脈瘤的效果比較及并發癥。方法:從2003～2008年近五年來我院收治的顱內動脈瘤54例,其中開顱夾閉24例(Hunt Hess分級Ⅰ～Ⅱ級20例,Ⅲ級2例,Ⅳ級2例),共26個動脈瘤。血管內栓塞30例(HuntHess分級Ⅰ～Ⅱ級23例,Ⅲ級5例,Ⅳ級2例),共31個動脈瘤。臨床結果按GOS進行評價。結果:兩組的良好率、并發癥及死亡率無顯著差別(Pgt;0.05)。隨訪平均12月,Ⅰ～Ⅱ級動脈瘤患者治療良好率為100.0％(43/43),并發癥發生率為4.7％(2/43),Ⅲ～Ⅳ級者分別為18.2％(2/11)和90.9％(10/11),兩者相差顯著(Plt;0.05)。結論:開顱夾閉和血管內栓塞治療顱內動脈瘤,二者療效相仿,各有優缺點。動脈瘤患者病情級別越高,治療效果越差。