【摘要】 目的 了解中藥千里光對金黃色葡萄球菌的抗菌作用，為治療金黃色葡萄球菌感染提供依據。 方法 將金黃色葡萄球菌在固體培養基上傳代培養后收集菌株，以一定濃度（菌落濃度為 3億/mL）用于腹腔注射。提取千里光水煎劑（2 g/mL）， 以其用于對小白鼠體內抗菌實驗。 結果 灌胃千里光水煎劑的實驗組死亡率顯著降低。 結論 中藥千里光對金黃色葡萄球菌有一定的抑制效果。【Abstract】 Objective To evaluate the antibacterial effect of traditional Chinese medicine Senecio against staphylococcus aureus, and to provide a experimental basis for the infectious treatment of staphylococcus aureus. Methods Staphylococcus aureus were cultured in solid medium and collected after uploading strains of a certain concentration (with the concentration of 300 million colonies/mL) for intraperitoneal injection. Senecio decoction extraction (2 g/mL) was used for mice in vivo antibacterial tests. Results The mortality rate in the experimental group with stomach perfusion of the Senecio water decoction decreased significantly. Conclusion The traditional Chinese medicine Senecio has a certain suppressive effect on staphylococcus aureus.
Objective To investigate the inhibition effect of silence of a disintegrin and metalloproteinase 17 (ADAM17) gene on proliferation and apoptosis of HT29 colon cancer cells and its possible mechanism. Methods HT29cells were divided into 3 groups: cells of interference group were transfected with recombinant lentivirus vector, cells of negative control group were transfected with negative recombinant lentivirus vector, and cells of blank control group were treated with PBS. The expression of ADAM17 mRNA was detected by real-time PCR, the expressions of ADAM17 protein, caspase3, protein kinase B (Akt), glycogen synthase kinase-3β (GSK3β), phospho-protein kinase B (P-Akt), phospho-glycogen synthase kinase-3β (P-GSK3β) protein were detected by Western blot method, the cell proliferation was detected by MTT assay, and the apoptosis rate was detected by Annexin V-FITC/PI cell death detection kit. Results Compared with the control group and the negative control group, the interference group was related to low expressions of ADAM17 mRNA and its protein, low optical density value at the same time point (24, 48, and 72 h), high apoptosis rate, high expression level of caspase3 protein, but low expression levels of P-Akt and P-GSK3β protein (P<0.05). Conclusion Silent ADAM17 gene could significantly induces apoptosis and inhibits the proliferation of HT29 cells, which maybe via inhibiting Akt/GSK3β signaling pathway.