【摘要】 目的 優選出紫冰栓中紫草油的最佳提取工藝。 方法 以左旋紫草素的含量為工藝考察指標，使用高效液相色譜建立左旋紫草素含量測定方法，采用L9（34）正交試驗法優化紫草油的提取條件。 結果 選用紫草，加8倍菜籽油，溫度140 ℃，加熱0.5 h為最優提取工藝。 結論 優選的提取工藝合理、可行，質量可控。【Abstract】 Objective To optimize the preparation process of extracting arnebiae oil from Zibing suppository. Methods The optimum preparation process of extracting arnebiae oil was investigated by L9(34) orthogonal design as the shikonin content index. Results The optimum technical condition: added eight times rape oil and cooked for half an hour with the temperature of 140 ℃. Conclusion The optimized preparation procedure is feasible and the quality of the product can be controled.
【摘要】 目的 考察用烏頭堿水解物、新烏頭堿水解物、次烏頭堿水解物的含量作為附片水煎劑及附片質量控制指標的可行性。 方法 采用高效液相色譜分析法，色譜柱為Shimpack CLC-ODS，以甲醇-乙酸銨溶液（0.2 mol/L）（200︰210）為流動相，流速1.0 mL/min，檢測波長為241 nm，對烏頭堿新，烏頭堿、次烏頭堿水解物的含量測定進行研究。 結果 3種水解物檢測方法測得水解物在進樣范圍有良好線性關系，重現性好，穩定性好，生藥提取時間宜選定為40 min，不同批號的附片中3種水解物含量差別較大。 結論 此方法簡便，使用新烏頭堿、烏頭堿、次烏頭堿的水解物作為含附片制劑的質量控制指標是可行的。【Abstract】 Objective To examine the feasibility of regarding the hydrolyzate from aconitine, new aconitine, and hypaconitine to be the decoction of radix aconite and the quality control index. Methods High performance liquid chromatographic method (HPLC) method was adopted. Shimpack CLC-ODS column with UV detection at 241 nm was used. The mobile phase consisted of (200∶210), and the flow rate was 1.0 mL/min. Results The content of the hydrolysis objects in the water decoction and toxicity were correlated with each other, and the contents of hydrolysis were significantly different among different groups of radix aconiti. Conclusion This method is simple, accurate and effective for content research of hydrolyzate from aconitine-type alkaloids in Monkshoo decoction of Radix Aconiti, and it is feasible to take it as the quality control index.
Objective To compare endoscopic sinius surgery plus middle meatus fenestration with endoscopic sinius surgery plus middle and inferior meatus fenestration for fungus ball maxillary sinusitis. Methods Applying a prospective randomized controlled trial, 80 patients with fungal ball maxillary sinusitis from January, 2010 to March, 2011 were collected and then divided into two groups, including experiment (40 cases) and control groups (40 cases). The trial group received endoscopic sinius surgery plus middle and inferior meatus fenestration, which the control group received endoscopic sinius surgery plus middle meatus fenestration. Then a follow-up was conducted from the end of surgery to February 28th, 2013. All patients took subjective and objective assessment before and after the surgery, including VAS, SNOT-20, Lund-Mackay CT system scores and Lund-Kennedy endoscopic mucosal score. Results with the trial group was superior to the control group in VAS score, SNOT rating and Lund-Kennedy mucosa score 6 months, 1 year, and 2 years after surgery (Plt;0.01). Lund-Mackay CT score of the control group was significantly higher than the trial group after 1 year of surgery (Plt;0.01). According to the Haikou standard to assess the efficacy of surgery, we found that the total effectiveness rate of the trial group (100.0%; recovery: 36 cases; improved: 4 cases) was higher than that of the control group (87.5%; recovery: 28 cases; improved: 4 cases), with a significant difference (P=0.021). Conclusion Endoscopic sinius surgery plus middle and inferior meatus fenestration with a lower reoccurrence rate is superior to endoscopic sinius surgery plus middle meatus fenestration for fungus ball maxillary sinusitis in clinical efficacy.