目的 研究質子泵抑制劑在反流性食管炎維持治療的臨床療效。 方法 將2009年3月-月門診及住院的121例反流性食管炎并胃鏡證實病灶已愈合，且停藥1周內癥狀又復發者，隨機分為A、B、C 3組，3組均選用蘭索拉唑。A組為蘭索拉唑15 mg，1次/d，早餐前服；B組為蘭索拉唑15 mg，1次/d，晚餐前服；C組蘭索拉唑15 mg，2次/d，餐前服。3組療程均為4周。療程結束后進行臨床癥狀療效評定，并予復查胃鏡，評價3組胃鏡下總有效率，并觀察3組不良反應。 結果 三種方案有效率分別為77.5%、95.0%、92.7%。 結論 晚餐前15 mg 1次/d的蘭索拉唑為反流性食管炎較佳維持治療方案。
Chemotherapy-related cognitive impairment (CRCI) is one of the treatment-related side effects in cancer patients, which can reduce patients’ participation in medical decision-making and treatment, seriously affecting their daily function and quality of life. This article reviews the definition, research status, and influencing factors of CRCI in lung cancer patients, in order to provide basis and ideas for the subsequent evaluation and management of CRCI in lung cancer patients, and promote the optimization and improvement of the overall rehabilitation process of lung cancer patients.
ObjectiveTo explore the feasibility of organoid culture derived from the patients with gastric cancer by suspension culture. MethodsThe fresh gastric cancer tissues of the 3 patients with gastric cancer were selected, which were digested with mixed enzymes and then made into cell suspensions, and were inoculated into ultra-low attachment plates to culture organoid by suspension. When the organoid growth was dense, the passage and freezing were carried out. The formation process of organoid was observed under the inverted microscope (IM). Further the consistency between the organoid and primary gastric cancer tissue was evaluated by hematoxylin-eosin (HE) and immunohistochemical (IHC) staining. ResultsIn this study, an organoid that could be passaged and frozen was successfully established in one patient. The results under the IM showed that the organoid was initially spherical in shape (cultured on day 5), then gradually became short rod-shaped on day 10, showed a branching like change on day 15, and formed irregular glandular tubular structures on day 20. The structures between the organoids and primary gastric cancer tissues were highly similar by HE staining. The IHC staining results showed that the expressions of low molecular weight cytokeratin (CK-LMW), p53, and Ki67 in the organoid and its corresponding primary gastric cancer tissue were basically the same. That was, the CK-LMW and p53 expressions were positive in the organoid and primary gastric cancer tissue, and the Ki67 was highly expressed (with a positive rate of approximately 70%). ConclusionBased on the preliminary research results of this study, it suggests that the suspension culture can be used to establish organoid derived from patients with gastric cancer, which is in accordance with primary tumor tissue at the tissue and cellular levels.