ObjectiveTo design a method for observing pulmonary microcirculation in vivo in a native tissue environment, due to the high incidence of lung diseases and the advantages of animal experiments in vivo.MethodsTen BALB/c male mice were randomly divided into group A and group B, with five mice in each group. A self-made apparatus was used to control the movement towards local lung tissues in order to get a stabilized observation plane, and then a 5-minute video was shot with laser confocal scanning microscope. During the filming, the mice in group A were injected with fluorescein isothiocyanate-dextran via the tail vein, and the mice in group B were injected with green fluorescent protein-platelets (extracted from the blood of tie2-cre&rosa26-tomato-EGFP transgenic black C57 male mice). The data of group A was used to observe the lungs perfusion and the damage to tissue by this method, and the data of group B was used to observe the movement of platelets.ResultsImage of lung structure obtained by this method was clear and stable. Mean areas of alveolus in an instant and at the 30th, 60th, 120th, 180th, and 300th second were (1 603±181), (1 588±183), (1 528±363), (1 506±353), (1 437±369), (1 549±307) μm2, respectively, and there were no significant differences between each time point (P>0.05). The video was smooth, the rapid movement of platelets was recorded and the particles were clear and without tailing; after the observation, hematoxylin-eosin staining showed no obvious damage to the lung tissue.ConclusionThe method can be used for the observation and research of the lung microcirculatory system in the living state of the mouse, and provides a methodological basis for studies of other lung diseases in vivo.
目的 運用營養風險篩查2002工具對普外科患者進行營養風險狀況和營養支持現狀調查。 方法 對2011年3月－8月在普外科新入院的520例患者進行營養風險篩查，判定是否存在營養風險，同時用已納入患者現有的臨床營養支持狀況，分析目前臨床營養支持的合理性。 結果 476例（91.5%）患者完成篩查。有營養風險者156例（32.8%），無營養風險者320例（67.2%）。在有營養風險患者中，實施臨床營養支持者有131例，占84.0%；無營養風險患者中，實施了營養支持者占40.3%。 結論 基層縣級醫院普外科入院患者營養風險發生率較高，并且臨床營養支持合理性尚待改善。