目的 研究尿標本中防腐劑鹽酸對24 h尿游離皮質醇測定的影響。 方法 收集2008年7月-2009年1月正常人、庫欣病患者及其他疾病患者的24 h尿液，混勻后，一部分濃鹽酸防腐，一部分未加鹽酸直接保存。電化學發光免疫分析法同步檢測尿游離皮質醇濃度。 結果 經配對 t 檢驗加濃鹽酸后的24 h尿游離皮質醇測值均高于未加酸者，比較有統計學意義（Plt;0.05）。加鹽酸和未加鹽酸所測尿游離皮質醇二者之間具有較好的相關性，相關系數 r =0.97，P lt;0.05。 結論 濃鹽酸防腐的標本24 h尿游離皮質醇測值較未加酸保存的標本高。因此，為了得到相對準確的值，更好地反映腎上腺實際分泌情況，測定24 h尿游離皮質醇的標本不應使用鹽酸防腐。
ObjectiveTo detect the 2019 novel coronavirus (2019-nCoV) in various biological specimens of novel coronavirus pneumonia (NCP), and preliminarily observe the status of 2019-nCoV in different systems of the body and its clinical significance.MethodsThe study design was a small-scale cross-sectional observational study. All the confirmed NCP cases being treated in the Second People’s Hospital of Yibin · West China Yibin Hospital, Sichuan University on February 2nd, 2020 were enrolled in this study. Two sets of primers were designed for 2019-nCoV-1ab and N regions using real-time reverse transcriptase-polymerase chain reaction (RT-PCR) assay. The 2019-nCoV in upper respiratory specimens, blood, feces and urine specimens of the NCP cases were detected on the single day.ResultsA total of 7 imported NCP cases (mild type) were included. The 7 patients were confirmed by the positive results of 2019-nCoV nucleic acid tests of upper or lower respiratory specimens between the 3rd day and the 7th day after fever onset, while 2 patients were found positive on the 3rd day after onset. The 2019-nCoV nucleic acid tests of the 7 patients were detected again on a single day between the 7th day and the 15th day after onset, and the results showed: the upper respiratory specimens of 5 patients were found negative (1 case was on the 7th day after onset); 2019-nCoV was not detected in the blood, feces or urine specimens of the 7 patients.ConclusionsFor mild type NCP patients, real-time RT-PCR test could detect 2019-nCoV between the 3rd day and the 7th day after onset, while 2019-nCoV might become negative since the 7th day after onset. 2019-nCoV was not detected in the blood, feces or urine of mild type NCP patients on the single day between the 7th day and the 15th day after onset. This study was only a preliminary observational study, which needed high-qualified studies to obtain more definitive conclusions.