Purpose To observe the expression of proliferating cell nuclear antigen（PCNA）and bcl-2 of cultured human retinal pigment epithelial cells（RPE）． Methods SABC techniques were applied for immunocytochemical staining of cultured RPE with mouse anti-human PCNA monoclonal antibody and rabbit antihuman bcl-2 antibodies． Results 31.2% and 50.6% cultured cells were positive to anti-human PCNA at 24h and 48h after seeding，respectively．The positive staining was mottled in the nucleus．positive staining for bcl was seen in 76%to 90% cells as fine granules scattered within the cytoplasm. Conclusion One half of cultured RPE expressed PCNA，indicating that the cells were in phase S of the cell cycle．Positive staining for bcl-2 appeared in much more RPE cells．These biological markers may be associated with the growth activity of cultured RPE． (Chin J Ocul Fundus Dis,1998,14:26-28)
Objective To investigate the spatial and temporal regulation effect of VEGF on human fetal retinal vascularization and angiogenesis. Methods The posterior segmental retinas from 54 human fetuses of the 9th week to the 40th week were studied by immunohistodhemistry standing for the expressions of VEGF and PCNA. Results 1. The distribution of VEGF espression was spiking and the peaks were during the 9th-13th and around the 26th week. 2. PCNA immunoreactivity was localized in spindle cells and vascular endothelial cells. The expression level was fluctuated during the developmental process. The peaks were during the 9th-13th and around the 21st week. In these periods, the spindle cells kept proliferating and differentiating, and remodelled subsequently to form the inner side retinal vessels. From the 26th or 34th week, the PCNA immununoreactivity is fully expressed in the vascular endothelial cells of the inner and outer margin of inner nuclear layer(INL) and kept to full terms. 3. Significant positive correlation were shown between the content of VEGF in the retina and that of PCNA in spindle cells and vascular endothelial cells(r=0.736,p＜0.01). Conclusion VEGF was positively involved in modulating human fetal retinal vascularization and angiogenesis. (Chin J Ocul Fundus Dis,1999,15:12-15)
摘要:目的: 探討PCNA,VEGF在胃癌組織中的表達關系及其意義。 方法 :免疫組織化學法檢測正常胃和胃癌組織中PCNA和VEGF的表達,并分析其與胃癌臨床病理特征的相關性。 結果 :胃癌組織中PCNA、VEGF的陽性表達率分別為850%、683%,二者在正常胃組織中均為隱性表達。PCNA的表達與腫瘤分期、淋巴結轉移有關(〖WTBX〗P lt;005),而與年齡、性別、腫瘤大小、組織分化程度無關(〖WTBX〗P gt;005);PCNA的表達與腫瘤分期、淋巴結轉移有關(〖WTBX〗P lt;005),而與年齡、性別、腫瘤大小、組織分化程度無關(〖WTBX〗P gt;005)。PCNA和VEGF在胃癌中的表達呈正相關。 結論 :PCNA和VEGF在胃癌組織中的高表達促進了胃癌的發生發展。Abstract: Objective: To study the significance and relationship of expression of proliferating cell nuclear antigen(PCNA) and vascular endothelial growth factor (VEGF) in gastric cancer. Methods : The expression of VEGF and PCNA in normal gastric tissues and gastric cancer tissues were detected by immunohistochemistry (SP),and clinic pathological correlation with gastric cancer was analyzed. Results : The positive exression rates of PCNA and VEGF in gastric cancer were 850%、683%, respectively, while negative expression was shown in normal gastric tissues. It was found that the expressions of PCNA and VEGF were both in significant relation to TNM stages and lymph node metastasis (P lt;005), but in no relation to age, gender, diameter of tumor and tumor cell differentiation (P gt;005). The expressions of PCNA and VEGF were positively correlated with each other. Conclusion : It was indicated that the highlevel expressions of PCNA and VEGF might corporately accelerate the progression of gastric cancer.
目的:初步探討伽瑪刀治療前后復發膠質母細胞瘤中P53及PCNA蛋白表達的變化及其臨床意義。方法:用免疫組化SP法檢測29例復發膠質母細胞瘤患者(伽瑪刀刀治療組12例,非伽瑪刀治療組17例)在初發和復發的腫瘤組織中P53蛋白和PCNA蛋白的表達。結果:兩組患者在性別,年齡,腫瘤部位及大小構成上差異無統計學意義(Pgt;0.05);兩組的復發時間的差異有統計學意義(P=0.0409lt;0.05);在伽瑪刀治療組P53及PCNA蛋白在復發膠質母細胞瘤中表達明顯降低(p53,t=3.915,P=0.02lt;0.05;PCNA,t=2.962,P=0.013lt;0.05);非伽瑪刀治療組p53及PCNA蛋白在復發膠質母細胞瘤中表達明顯增加(p53,t=-5.926,P=0.000lt;0.05;PCNA,t=-5.160,P=0.000lt;0.05);P53及PCNA蛋白在伽瑪刀治療組和非伽瑪刀治療組的表達變化有統計學意義(p53,t=-5.577,P=0.000lt;0.05PCNA,t=-5.542,P=0.000lt;0.05);在伽瑪刀治療組及非伽瑪刀治療組,P53蛋白和PCNA蛋白的陽性表達率不存在明顯的相關性(伽瑪刀治療組,r=-0.085,P=0.792gt;0.05非伽瑪刀治療組,r=0.450,P=0.07gt;0.05)。結論:P53及PCNA蛋白的異常表達與膠質母細胞瘤的復發有關,伽瑪刀治療膠質母細胞瘤瘤可能通過抑制P53及PCNA蛋白表達而起作用。