OBJECTIVE: The review the effect of cytokines on repair of tendon injury and the relevant mechanism. METHODS: By broadly consulting recent issues about cytokines involved in tendon repair, a variety of cytokines with effects in repairing injured tendon was made and the possible mechanisms were summarized, with unsolved problems discussed. RESULTS: There were many cytokines participated in the procedure of tendon repair, among which insulin-like growth factor (IGF-1), transforming growth-beta 1 (TGF-beta 1) played significant roles. Most of the relevant researches were limited in experimental study in vitro. CONCLUSION: Cytokines possibly can accelerate tendon repair and show great potentials in future clinical application.
目的 總結直腸超聲引導下經會陰前列腺12點穿刺活檢術圍手術期的護理經驗。 方法 對2010年6月－2012年10月行直腸超聲引導下經會陰前列腺12點穿刺活檢術932例患者圍手術期護理措施及要點予以回顧分析。 結果 932例患者順利完成穿刺活檢，術后除1例患者出現迷走神經反射綜合征、經及時搶救緩解外，無其他并發癥發生。 結論 直腸超聲引導下經會陰前列腺12點穿刺活檢術具有安全簡便、無需腸道準備、并發癥少、準確率高等優點，充分的圍手術期護理是該活檢術順利完成的重要因素與環節。
【摘要】 目的 探討超聲引導下導絲定位在不可觸及的乳腺病灶切除中的應用價值。 方法 對2005年1月－2010年9月127例女性患者的137個乳腺病灶（臨床捫診均為陰性），在超聲引導下進行導絲定位，后進行外科切除活檢，并對相關資料進行回顧性分析。 結果 137個病灶的組織學結果中，良性病灶101個（73.7%）、高風險病灶27個（19.7%）和癌9個（6.6%）。9個癌中3個為導管原位癌，6個為浸潤性導管癌（大小11～19 mm，平均14.2 mm）。超聲引導下導絲定位的時間為3～15 min，平均6 min；無血腫、導絲脫落及折斷等并發癥發生。外科手術切除時間20～40 min，平均30 min。 結論 超聲引導下進行導絲定位安全、迅速，能協助外科手術進行準確的活檢和切除。【Abstract】 Objective To determine the application value of ultrasound-guided wire localization in surgical excision of non-palpable breast lesions. Methods Between January 2005 and September 2010, 127 women with 137 non-palpable breast lesions underwent surgical excision at West China Hospital. Palpation results for all the lesions were negative. Wire localization guided by ultrasound was performed before operation and biopsy. Related imaging studies and medical records were reviewed retrospectively. Results Histological findings showed there were 101 benign lesions (73.7%), 27 high-risk lesions (19.7%), and 9 carcinomas (6.6%). Among the 9 carcinomas, 3 were ductal carcinoma in situ, and 6 were infiltrating carcinoma (with their size ranged from 11 to 19 mm averaging at 14.2 mm). The time of performing ultrasound-guided wire localization was from 3 to 15 minutes averaging at 6. No complications like hematoma, wire fragments, and wire breakage occurred in all cases. The surgical excision time ranged from 20 to 40 minutes averaging at 30. Conclusions Ultrasound-guided wire localization can be performed quickly and safely for the cases of non-palpable breast lesions. It is useful in assisting surgical excision and biopsy.
Objective To discuss the nursing measures for thyroid nodule patients who undergo core-needle biopsy (CNB) guided by ultrasound. Methods We retrospectively analyzed the experiences and main points of nursing for 1 900 thyroid nodule patients who underwent CNB guided by ultrasound between June 2010 and May 2014. Results All the 1 900 patients underwent CNB successfully. The nursing time was between 5 and 15 minutes, averaging (8.0±3.7) minutes. Complications included hematoma in 25 patients (1.3%) and needle syncope reaction in 30 patients (1.6%), which were cured through symptomatic treatment. No complications such as nerve injury, anesthesia accident or death occurred. No medical disputes happened due to specimen errors or loss. The success rate of specimen collection was 98.4% (1 870/1 900), and the diagnostic accuracy was 95.3% (1 812/1 900). Conclusions Ultrasonography-guided CNB is a safe and reliable operation with a high success rate, high diagnosis accuracy and few complications. Being familiar with the process of nursing cooperation and correct disposal and transfer of biopsy specimens are crucial for successful CNB in patients with thyroid nodules.
Objective To evaluate the diagnostic accuracy of enzyme immunoassay (EIA) for chlamydia trachomatis (CT). Methods The diagnosis trials on EIA for CT were searched in the databases such as PubMed (1966 to Dec. 2011), The Cochrane Library (Issue 12, 2011), EMbase (1974 to Dec. 2011), CNKI (1994 to Dec. 2011), VIP (1989 to Dec. 2011) and CBM (1978 to Dec. 2011), meanwhile the manual and other retrieves were also conducted. Two reviewers evaluated the quality of the included trials according to the quality assessment of diagnostic accuracy studies (QUADAS), and then meta-analysis was performed using Meta Analyst and RevMan 5.0 software. Results A total of 17 trials involving 9 461 participants were included. The results of meta-analysis showed that the weighted sensitivity, specificity, positive likelihood ratio, negative likelihood ratio, diagnostic odds ratio, and the area under SROC curve were 0.847 (95%CI 0.571 to 0.995), 0.964 (95%CI 0.890 to 0.994), 25.972 (95%CI 18.587 to 36.293), 0.156 (95%CI 0.114 to 0.212), 228.875 (95%CI 127.136 to 412.028), and 0.953, respectively. Conclusion EIA for CT has higher sensitivity and specificity, so EIA is recommended for preliminary screening CT and diagnosing the highly suspected cases or the patients without obvious signs and symptoms.
Objective To assess the methodological quality of systematic reviews or meta-analyses of intervention published in the Chinese Journal of Evidence-Based Medicine, so as to provide evidence for improving the domestic methodological quality. Methods The systematic reviews or meta-analyses of intervention published from 2001 to 2011 were identified by searching the Chinese Journal of Evidence-Based Medicine. The methodological quality of included studies was assessed by AMSTAR scale. The Excel software was used to input data, and Mata-Analyst software was used to conduct statistical analysis. Results A total of 379 studies were included. The average score of AMSTAR was 6.15±1.35 (1.5-9.5 point). Just some items of AMSTAR scale were influenced by the following features of included studies: publication date, funded or not, number of author, author’s unit, and number of author’s unit. The total AMSTAR score of studies published after 2008 was higher than those published before 2008 (P=0.02), but the improvement of methodological quality was limited. While the total AMSTAR score of studies published by 3 or more than 3 authors were higher than those published by 2 or less than 2 authors (P=0.04). Conclusion The methodological quality of the included studies published in the Chinese Journal of Evidence-Based Pediatrics is uneven. Although the methodological quality improves somewhat after the publication of AMSTAR scale, there is no big progress, so it still needs to be further improved.
Objective To assess the reporting quality of systematic reviews/meta-analyses related to interventions published in Chinese Journal of Evidence-Based Medicine by PRISMA guidelines, and to analyze its influencing factors. Methods The systematic reviews/meta-analyses related to interventions were searched in the Chinese Journal of Evidence-Based Medicine from its inception to 2011. The quality of the included reviews was assessed in accordance with the PRISMA checklist. Based on the degree of conformity with each criterion of PRISMA, the reviews were scored as “1”, “0.5” or “0” orderly. The data were put into Excel, and the Meta-analyst software was used for statistical analysi. Results Among all literature in the volume 11 (95) of the Chinese Journal of Evidence-Based Medicine from 2001 to 2011, a total of 379 studies were included, and the number of publication showed a yearly rising trend. The PRISMA scale score ranged from 8.5 to 26 (X±SD) was 19.97±3.15. Among all studies, 25 (6.60%) scored 21-27 points, which were regarded as the complete reporting; 226 (59.63%) scored 15-21 points, regarded as relatively complete reporting; and 128 (33.77%) scored less than 15 points, regarded as serious lack of information. The results of stratified analysis showed that, both the issue of PRISMA and fund support could improve the reporting quality, with a significant difference (Plt;0.05); and authors more than 3, authors from universities, and authors from more than 2 institutions could improve the reporting quality, but without a significant difference (Pgt;0.05). Conclusion The overall reporting quality of systematic reviews/meta-analyses related to interventions published in the Chinese Journal of Evidence-Based Medicine is poor, and it is influenced by the factors of protocol and registration, risk of bias across studies, other analyses, and fund support, which have to be taken seriously. The reasonable utilization of the PRISMA checklist will improve the reporting quality of systematic reviews/meta-analyses.
Objective The current study aimed to compare the differences in clinical characteristics and prognosis of elderly patients with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection between patients who were vaccinated and those not and to explore the clinical significance of vaccination for the elderly.Methods A total of 92 elderly patients (≥60 years old) with SARS-CoV-2 infection who were admitted to Chengdu Public Health Clinical Center from December 10, 2020, to May 2, 2022, were included, and they were grouped according to whether vaccinated. The differences in clinical manifestations, laboratory examinations, imaging, treatment, prognosis, hospitalization time, and nucleic acid conversion time between the two groups were compared in this study. Results A total of 92 elderly patients were included, with a male-to-female ratio of 1.3:1, and a median age of 66 (62, 71) years old. There were 79 patients in the vaccinated group and 13 in the unvaccinated group. The positive rate of total SARS-CoV-2 antibody in 92 patients was 91.3%, and those of IgG and IgM of SARS-CoV-2 antibody were 89.1% and 37%, respectively. The positive rates of total SARS-CoV-2 antibody and IgG of SARS-CoV-2 antibody in the vaccinated group were higher than those in the unvaccinated group (97.5% vs. 53.8%, 96.2% vs. 46.2%) (P<0.01), and COI values of total antibody, IgM and IgG were higher than those of unvaccinated group (P<0.01). The proportions of the initial symptoms of sputum, ground-glass opacity or patchy opacity involving both lungs in chest CT in the unvaccinated group were higher than those in the vaccinated group (P<0.05). The white blood cell counts and platelet counts in the vaccinated group were higher than those in the unvaccinated group, whereas the prothrombin time and D-dimer were lower than those in the unvaccinated group (P<0.05). COI values of total antibody in the 3-doses group were higher than those in the 2-doses group, and the white blood cell counts in the 3-doses group were higher than those in the 2-doses group (all P<0.05). During hospitalization, asymptomatic infection (58.2%) and general type (53.8%) was the most common in the vaccinated and unvaccinated group, respectively. Patients in the unvaccinated group were more likely to progress to severe status than the vaccinated group during hospitalization (15.4% vs. 0%, P=0.019). The unvaccinated group received more treatments of intravenous immune globulin, non-invasive and invasive mechanical ventilation, plasma after immunization of vaccine and convalescent plasma of SARS-CoV-2 infected patients than those of the vaccinated group (P<0.05). Patients in the unvaccinated group developed a higher proportion of respiratory failure, secondary infection, acute respiratory distress syndrome, and heart failure than in the vaccinated group (P< 0.05). The median lengths of hospitalization and nucleic acid conversion in the unvaccinated group were 22 (7, 32) days and 13 (2, 20) days, which were longer than those in the vaccinated group [8 (7, 12) days, 2 (2, 7) days] (all P<0.05). Conclusions Vaccination of SARS-CoV-2 can improve the positive rate of total SARS-CoV-2 antibody and IgG of SARS-CoV-2 antibody in elderly patients with SARS-CoV-2 infection, milder disease status, and can shorten the time of hospitalization and nucleic acid conversion. These results suggest that the COVID-19 vaccine can reduce the disease and improve the prognosis in the elderly.