Objective To investigate the effect of interleukin-10 （IL-10） gene transfer on expression of CD44, selectin-E, lymphocyte function associated antigen-1 （LFA-1）, vascular cell adhesion molecule-1 （VCAM-1） in mice heart transplantation rejection. Methods Model of mice cervical heterotopic heart transplantation was set up, 96 mice were divided into three groups with random number table, control group： heart transplantation between C57 mice; transplant group： heart from BALB/C mice transplant to C57 mice; IL-10 group： IL-10 was transfected on BALB/C mice isolated heart for 1 hour, then transplanted to C57 mice. The messenger ribonucleic acid （mRNA） level expression of CD44 ,selectin-E ,LFA-1 ,VCAM-1 and IL-10 were measured by reverse transcription-polymerase chain reaction （RT-PCR） at the 5th day after transplantation. Results The mRNA level expression of CD44, selectin-E ,LFA-1 ,VCAM-1 in transplant group were significantly increased than those in control group （P〈0.01）. The mRNA level expression of CD44, selectin-E, LFA-1 ,VCAM-1 in IL-10 group were significantly decreased than those in transplant group （P〈0.01）. Conclusion IL-10 gene transfer is able to decrease the expression of CD44, selectin-E,LFA-1 ,VCAM-1 and suppress the heart transplantation rejection in mice.
Objective To investigate the optimal surgical approach for bilateral inguinal hernias and complex (recurrent and compound) inguinal hernia. Methods Data of eighty patients with bilateral inguinal hernias and (or) complex inguinal hernias treated by tension less hernia repair between Feb. 2007 and Jun. 2010 in Sichuan Provincial People’s Hospital were analyzed. Preperitoneal approach through inferior abdominal median incision was applied in the repair with local, lumbar or epidural anesthesia. Results Operation time was （30±10.2） min in unilateral hernia, （50±17.5） min in bilateral hernia. There was no ischemic orchitis or pain case after operation. Within 3 months following up, no recurrence occurred. Conclusion The preperitoneal approach through inferior abdominal median incision for inguinal hernia repair is proved to be effective, safe, and convenient, and especially fit for bilateral hernias and complex hernia.
目的 通過非小細胞肺癌（NSCLC）圖像引導放射治療（IGRT）過程中，每次治療前獲取的錐形束CT圖像，動態觀察腫瘤體積的變化。 方法 2009年2月－2010年8月18例周圍型NSCLC患者接受IGRT。每次治療前進行千伏級錐形束CT（KV-CBCT）圖像的采集。每周在KV-CBCT圖像上勾畫腫瘤靶區并計算靶區體積，經統計后分析腫瘤治療過程中體積的變化。 結果 治療開始時平均體積為28.5 cm3（2.5～109.1 cm3），治療結束時平均體積為17.1 cm3 （1.4～73.4 cm3）。平均退縮率為35.9%（3.9%～68.9%），平均每天的退縮率為1.5% （0.1%～5.4%）。治療結束時，0例病灶完全消退，1例部分消退，10例微小消退，7例穩定。 結論 治療過程中，NSCLC腫瘤的退縮可以通過KV-CBCT進行觀察。當病灶為周圍型時，能對腫瘤體積的變化進行客觀有效的評價。放射治療過程中腫瘤的體積改變具有很大的異質性，腫瘤在治療過程中體積均有一定的退縮，但治療結束時大多數病灶僅為微小消退或穩定。