ObjectiveTo systematically review the efficacy of exercise intervention on smoking cessation. MethodsCNKI, WanFang Data, VIP, Web of Science, PubMed and EMbase databases were electronically searched to collect randomized controlled trials (RCTs) and randomized cross controlled trials (RCDs) on exercise intervention for smoking cessation from inception to September 2021. Two reviewers independently screened literature, extracted data and assessed the risk of bias of the included studies. Then, meta-analysis was performed using Stata 12.0 software. ResultsA total of 47 studies (35 RCTs and 12 RCDs) involving 5 130 subjects were included. The results of meta-analysis showed that acute exercise could significantly reduce the quitters’ desire to smoke (P<0.05), alleviate most of the withdrawal symptoms, and the effect of acute exercise was maintained for at least 30 minutes. Periodic exercise significantly reduced 7-day point abstinence and sustained abstinence rates in ex-smokers (P<0.05), and the effect of periodic exercise was maintained for at least 12 weeks, however, depression and stress were not found to be effectively relieved, and mood was not found to be significantly improved (P>0.05). ConclusionExercise intervention to quit smoking has a positive effect, however, more high-quality studies are needed to verify the above conclusion.
【摘要】 目的 原肌球蛋白是肌球蛋白主要相關蛋白之一，在細胞骨架與運動中起著重要的作用。探討原肌球蛋白在增生性瘢痕中的作用，有助于揭示瘢痕攣縮的產生機制。 方法 收集2006年3月-2008年7月48例患者不同時期增生與非增生瘢痕組織標本，利用基因芯片篩選出的瘢痕相關基因——原肌球蛋白基因特異片段，制備成寡核苷酸探針與瘢痕組織切片進行原位雜交。同時，將各標本進行原代細胞培養，制備成纖維細胞爬片，進行原位雜交。 結果 原肌球蛋白基因在3、6個月的增生性瘢痕中表達均明顯強于9、12個月增生性瘢痕及非增生性瘢痕，陽性細胞比例也高于9、12個月增生性瘢痕及非增生性瘢痕。 結論 瘢痕增生攣縮與細胞骨架運動的相關基因存在密切關系，而原肌球蛋白可能在其中起著重要作用。【Abstract】 Objective Tropomyosin is one of the proteins of cytoskeleton and cell movement. The aim of this study is to investigate the effect of gene expression of fibroblast tropomyosin on the formation and contraction of hypertrophic scar. Methods According to the results of differently expressed genes, in hypertrophic scar by gene microarray, topomyosin, one of the most important genes, was selected and made into oligonucleotide probe. Twenty-four hypertrophic scars and 24 non-hypertrophic scars and 12 normal skins were used and these scar were taken on 3, 6, 9, and 12 months after burned between March 2006 and July 2008. Frozen section and cultured fibroblasts were made to detect the expression of the gene by in situ hybridization. Results Expression of tropomyosin was defected in scar tissue, but those in the hypertrophic scar on 3 and 6 months after burn were significantly ber than those in the hypertrophic scar on 9 and 12 months after burn and non-hypertrophic scar. Conclusion Overexpression of cytoskeletal relative genes causes the contraction of scar and tropomyosin acts leading and key functions.