Objective To investigate the feasibility of repairing thyroid cartilage defects by implantation of chondrocyte-allogenous acellular cartilaginousmatrix(chondrocyte-ACM) composite in rabbits. Methods The thyroid chondrocyteswere isolated and co-cultured in vitro with allogenous acellular cartilaginousmatrix(ACM) to form the chondrocyte-ACM composite. The composite was analyzed histologically and was used to repair defects of thyroid cartilage. Eighteen New Zealand adult rabbits were made the defect models of thyroid cartilage at the two sides and divided into three groups. The defects were repaired with chondrocyte-ACM composite in the experimental group(n=6), with simple ACM in the ACM group (n=6)and without any material in the control group(n=6). The animals were sacrificed at 8 weeks after operation. The specimens were evaluated histologically. Results In vitro, the growth of chondrocytes was observed on the surface of allogenous acellular cartilaginous matrix and no chondrocytes grew inside the matrix. The defect filled with muscle and connective tissues in control group; the lymphocyte infiltration was observed in the matrix and no new cartilage formationoccurred at 8 weeks after operation in simple ACM group and experimental group.So the defect repair of rabbits thyroid cartilage failed. Conclusion The allogenous acellular cartilaginous matrixfailed to serve as a scaffold for chondrocytes both in vitro and in vivo. The allogenous acellular cartilaginous matrixshould be improved.
【Abstract】 Objective When knee medial collateral ligament (MCL) rupture, the upper surface of medial meniscus is exposed totally, like the gulf panoramic, which is called “panoramic views of the bay sign” or the “bay sign”. To investigate the reliability and significance of the “bay sign” in diagnosis of knee MCL rupture under arthroscope. Methods Between March 2007 and March 2011, 127 patients with knees injuries were divided into the observation group (n=59) and control group (n=68) based on the MRI results. In the observation group, 59 patients had MCL rupture by MRI, including 12 cases of MCL injury alone, 16 cases of MCL injury with lateral meniscus torn, 27 cases of MCL injury with anterior cruciate ligament (ACL) injury, 3 cases of MCL injury with ACL and posterior cruciate ligament (PCL) injury, and 1 case of MCL injury with patellar dislocation; there were 38 males and 21 females with an average age of 23.2 years (range, 16-39 years). In the control group, 68 patients had no MCL rupture by MRI, including 38 cases of ACL injury, 4 cases of ACL and PCL injury, and 26 cases of ACL and lateral meniscus injury; there were 45 males and 23 females with an average age of 31.8 years (range, 25-49 years). The “bay sign” was observed under arthroscope in 2 groups before and after operation. Results The positive “bay sign” was seen under arthroscope in the patients of the observation group before MCL repair; the “bay sign” disappeared after repair. No “bay sign” was seen in patients of the control group before and after ACL reconstruction. Conclusion The “bay sign” is a reliable diagnostic evidence of MCL injury. It can be used as a basis to judge the success of MCL reconstruction during operation.
目的 觀察右美托咪啶復合舒芬太尼用于經腹子宮全切術后患者自控靜脈鎮痛（PCIA）的效果。 方法 2011年3月－2012年6月選擇經腹子宮全切術患者90例，年齡39～68歲，體重48～72 kg，美國麻醉醫師協會分級Ⅰ～Ⅱ級。采用隨機數字表法，將患者隨機分為3組，每組各30例。于手術結束即刻行PCIA。對照組（C組）采用舒芬太尼150 μg+昂丹司瓊12 mg；S1組采用右美托咪定200 μg+舒芬太尼100 μg+昂丹司瓊12 mg；S2組采用右美托咪定200 μg+舒芬太尼150 μg+昂丹司瓊12 mg。3組均用生理鹽水稀釋至100 mL，負荷劑量均為舒芬太尼0.1 μg/kg，靜脈鎮痛泵背景輸注速度2 mL/h，自控給藥劑量0.5 mL，鎖定時間15 min。記錄術后6、12、24和48 h Ramsay鎮靜評分和視覺模擬評分（VAS），記錄不良反應發生情況和患者對術后鎮痛的滿意度。 結果 3組患者均能獲得較好的鎮痛效果。其中C組VAS評分較低，但惡心、嘔吐、皮膚瘙癢發生率升高；與C組相比，Sl組和S2組Ramsay鎮靜評分升高，惡心、嘔吐、皮膚瘙癢發生率降低，患者滿意度升高。S1組患者滿意度最高；S2組VAS評分最低。3組均未發生心動過緩、低血壓、過度鎮靜和呼吸抑制。 結論 右美托咪啶可增加經腹子宮全切術患者術后舒芬太尼自控靜脈鎮痛的效果，提高患者滿意度，降低不良反應。
Objective To observe whether apoptosis was involved in cells of aspiration fluid from vitrectomy for proliferative vitreoretinopathy(PVR),and whether there was an association with expression of Fas antigen(Fas )and Fas ligand (FasL). Methods Cytocentrifuge slides of 11 fresh vitreous specimenｓ of PVR were prepared to be stained by TUNEL met hod for detection of apoptosis and by immunohistochemical technique for detection of Fas,FasL，and cytokeratin (CK),a cell-type specific antigen. Results Fas and FasL were expressed in normal human retina.Fas,FasL,CK，and apoptosis were found in all preparations.TUNEL-positive cells were 20.53% in total cells.70.35%,51.58%，and 82.97% of cells highly expressed Fas,FasL，and CK,respectively.The linear correlation coefficient of Fas and apoptosis was 0.99(Plt;0．001). Conclusion Vitrectomy specimens of PVR showed expression of Fas，FasL，and apoptosis.Prominent Fas and FasL expressions may be associated with apoptosis of proliferating retinal pigment epithelial cells in the vitreous of PVR. (Chin J Ocul Fundus Dis,1999,15:78-80)