Objective To investigate expressions of EphA2 and EphrinA1 in invasive ductal carcinoma of breast and to explore their clinical significances. Method The protein and mRNA expressions of EphA2 and EphrinA1 in 30 breast fibroma tissues, 30 breast cystic hyperplasia tissues, and 100 invasive ductal carcinoma of breast tissues were detected by immunohistochemistry andin situ hybridization respectively, and correlation between them and relations between their expressions in invasive ductal carcinoma of breast tissues and clinicopathologic factors were analyzed. Results ① The results of the immunohistochemistry andin situ hybridization tests showed that the protein and mRNA expressions of EphA2 and EphrinA1 in the invasive ductal carcinoma of breast tissues were significantly higher than those in the breast fibroma tissue (P<0.001) and breast cystic hyperplasia tissue (P<0.001). ② The positive expressions of EphA2 and EphrinA1 protein and mRNA were associated with the lymph node metastasis, histological grade, and TNM stage (P<0.05), in other words, which in the invasive ductal carcinoma of breast patients with lymph node metastasis, high histological grade, and high TNM stage were higher. However, which were not associated with the age and the tumor diameter (P>0.05). ③ The positive protein expressions or positive mRNA expressions in the invasive ductal carcinoma of breast tissues all had positive correlations between the EphA2 and the EphrinA1 (protein:rs =0.999,P<0.01; mRNA:rs =0.942,P<0.01). Conclusions EphA2 and EphrinA1 might be involved in carcinogenesis and development procedures of invasive ductal carcinoma of breast. Combined detection of EphA2 and EphrinA1 could help to predict clinical and pathologic characteristics of invasive ductal carcinoma of breast. They might provide a new target for clinical medication, prognosis, and targeted therapy.
ObjectiveTo investigate the effects of FTY720-P on EphA2-EphrinA2 bidirectional signaling in osteoclasts.MethodsMurine RAW264.7 macrophages were induced into osteoclasts by dexamethasone and 1α, 25-dihydroxyvitamin D3, and identified by tartrate resistant acid phosphatase (TRAP) staining. Then, the osteoclasts were divided into 2 groups. The osteoclasts were treated with 400 ng/mL FTY720-P in experimental group and without FTY720-P in control group, respectively. After 48 hours of culture, the cells in 2 groups were detected by real-time fluorescent quantitative PCR, Western blot, and immunofluorescence staining. The expressions of EphA2, EphrinA2, RhoA, and the bone reconstruction associated proteins［bone morphogenetic protein 2 (BMP-2) and transform growth factor β1 (TGF-β1)］were analyzed and compared.ResultsRAW264.7 cells were successfully induced into osteoclasts identified by TRAP staining. Compared with control group, the relative expressions of EphA2 and EphrinA2 mRNAs and proteins in experimental group significantly decreased after 48 hours (P<0.05), and the relative expression of RhoA protein also significantly decreased (P<0.05). The relative expressions of BMP-2 and TGF-β1 mRNAs were significantly increased (P<0.05), and those protein expressions were enhanced.ConclusionFTY720-P can down-regulate the expression of RhoA and promote the expressions of TGF- β1 and BMP-2 by affecting the transduction of EphA2-EphrinA2 bidirectional signaling in osteoclasts.
探討宮頸癌中EphA2的表達及其臨床意義。方法:應用免疫組織化學方法檢測10例慢性宮頸炎、25例宮頸上皮內瘤樣變（CIN）、50例宮頸癌（鱗癌41例、腺癌9例）中EphA2的蛋白表達。并對50例宮頸癌患者進行8～46月隨訪，分析EphA2在宮頸癌中的表達與患者預后的關系。結果:慢性宮頸炎、CIN、宮頸癌組織中EphA2蛋白陽性表達的差異有統計學意義（P＜0.05）。EphA2蛋白在宮頸癌中的表達與患者年齡、FIGO分期、病理類型無關，與病理分級、有無淋巴結轉移有密切相關性，且EphA2蛋白表達越強，患者生存率越低。結論：EphA2與宮頸癌的侵襲、轉移密切相關，可望作為判斷宮頸癌患者預后的參考指標。