Although transcranial magnetic stimulation (TMS) is widely used in neuromodulation, conventional TMS struggles to achieve both depth and focal specificity. Temporal interference TMS (TI-TMS) offers a promising approach to enhance stimulation depth while reducing the focal area; however, current research remains largely simulation-based, with limited studies on system implementation and experimental validation in rodent deep brain regions. To address this, we developed a TI-TMS system based on a realistic mouse head model using finite element simulation. Electrophysiological recordings of local field potentials (LFPs) in the ventral hippocampal (vHPC) formation were performed to evaluate changes in θ rhythm power spectral density (PSD) and θ-γ phase-amplitude coupling (PAC) following stimulation. The results demonstrated that TI-TMS enhanced θ rhythm power and strengthened θ-γ PAC, indicating effective modulation of deep brain regions. This study establishes a functional TI-TMS system capable of effectively stimulating deep vHPC, providing an experimental basis for its application in precise neuromodulation of subcortical brain areas.