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Changes of the Level of G Protein in Newborn Guinea-pig Myocardium Undergoing Global Ischemic Reperfusion

ObjectiveTo study the changes of levels of α subunits of stimulatory (Gsα) and inhibitory guanine nucleotide binding protein (Giα) in newborn guinea pig (0 2 days old) myocardium undergoing global ischemic reperfusion, and influences on the changes by St.Thomas Ⅱ and cold blood cardioplegic solution.MethodsThirty newborn guinea pigs were randomly assigned to three groups. GroupⅠ ( n = 10): the newborn hearts suffered by hypothermic global ischemia; group Ⅱ( n =10): the newborn hearts arrested by St. Thomas Ⅱ , and group Ⅲ ( n = 10): the newborn hearts arrested by cold blood cardioplegic solution. Levels of Gsα and Giα were investigated with Western blot analysis.ResultsNo differences of levels of Gsα and Giα were found in three groups before ischemia ( P gt;0.05). The level of Gsα after ischemia was significantly decreased than before ischemia in groupⅠand group Ⅱ ( P lt; 0 01), whereas no pronounced changes in group Ⅲ ( P gt;0.05) were noted after ischemia. The level of Gsα in group Ⅲ was not significantly changed after reperfusion compared with before ischemia( P gt;0 05), and it was much higher than those in groupⅠand group Ⅱ ( P lt; 0 01). Level of Giα was found not markedly changed in group Ⅲ after reperfusion compared with that before ischemia, but was notable higher in groupⅠand group Ⅱ( P lt;0.01). ConclusionsSignificant decrease of level of Gsα, whereas marked increase of level of Giα are found in myocardium of newborn guinea pig undergoing hypothermic (20℃) ischemic reperfusion. No impact of St. Thomas Ⅱ on these changes is verified, but recovery to the level of Gsα and Giα before ischemia is achieved by cold blood cardioplegic solution after ischemia and reperfusion. Unbalance between Gsα and Giα is the one of the mechanisms of ischemic reperfusion injury for immature myocardium.

月見草油預防豚鼠膽固醇結石的實驗研究

肺部豚鼠耳炎諾卡菌感染一例并文獻復習

目的 總結肺豚鼠耳炎諾卡菌病的臨床特點，以提高診斷和治療水平。方法 回顧性分析1例肺豚鼠耳炎諾卡菌病的發病過程、臨床表現、胸部影像學和病原學檢查及診治過程。在PubMed數據庫以“pulmonary”和“Nocardia otitidiscaviarum”為檢索詞進行檢索，以“肺豚鼠耳炎諾卡菌”為檢索詞在中國知網、萬方數據庫中檢索年2016—2021年豚鼠耳炎諾卡菌病病例，并復習相關文獻。結果 患者女，44歲，因“咳嗽、咳痰及痰中帶血伴發熱1 d”于2020年7月25日就診。入院診斷為重癥肺炎，既往有支氣管擴張病史，影像學檢查顯示肺部斑片狀陰影并空洞，通過痰涂片、痰培養及肺泡灌洗液病原學檢查結果提示肺豚鼠耳炎諾卡菌。感染急性期予美羅培南聯合去甲萬古霉素治療，后于院外口服復方磺胺甲噁唑治療3個月，隨訪1年未復發。結論 肺部豚鼠耳炎諾卡菌病臨床少見，其臨床及影像學等表現無特異性，確診需依靠病原學檢查，首選磺胺類藥物治療。早診斷、早治療有助于改善肺豚部鼠耳炎諾菌病患者的預后。