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降主動脈置換治療局部晚期食管癌一例

LncRNA MIR223HG regulates ATM expression affecting proliferation, migration and apoptosis of lung adenocarcinoma cells

Objective To investigate the molecular mechanisms by which the long non-coding RNA (lncRNA) MIR223HG affects the proliferation, migration and apoptosis of lung adenocarcinoma cells. MethodsDNA damaging agent Zeocin was used to treat human embryo lung cell (MRC-5) and lung cancer cell (A549 and H1299), and the expression of MIR223HG was tested by quantitative real-time polymerase chain reaction (qRT-PCR) analysis. Moreover, the ataxia-telangiectasia mutated (ATM) protein and ATM pathway downstream factor Cell cycle checkpoint kinase 2 (Chk2), p53 tumor suppressor protein (p53) in the lung cancer cell (A549 and H1299) with Zeocin were also tested by qRT-PCR. Cell transfection and Transwell migration assay, colony formation assays, apoptosis assays were performed to verify the role of ATM in the expression of MIR223HG in lung adenocarcinoma. ResultsThe expression of MIR223HG was reduced markedly in the lung cancer cells (A549 and H1299) compared with human embryo lung cell (MRC-5) after treated with Zeocin. ATM protein and its downstream factors Chk2, p53 involved in the process, and ATM regulated the expression of MIR223HG in the lung cancer cells with Zeocin. Futhermore, ATM joined in the processes that MIR223HG regulated the lung cancer cells proliferation, migration and apoptosis. Conclusions The expression of MIR223HG is related to the DNA damage response in the lung cancer, and MIR223HG regulates lung cancer cells proliferation, migration and apoptosis by ATM/Chk2/p53 pathway. MIR223HG may be a potential therapeutic target for lung adenocarcinoma treatment.

改良Nissen手術在滑動性食管裂孔疝治療中的應用

目的評價改良Nissen手術在滑動性食管裂孔疝治療中的應用價值。 方法回顧性分析自2001年6月至2013年5月蚌埠醫學院第一附屬醫院52例滑動性食管裂孔疝經手術治療患者的臨床資料，其中男27例、女25例，平均年齡62.13（35～84）歲。所有患者術前均行上消化道X線鋇餐造影、胃鏡檢查確診為滑動性食管裂孔疝，均行改良Nissen手術，即胃底上提至賁門口上方2～3 cm，包繞雙層縫合固定食管180°，并置于膈肌下方。分別于術后3個月、6個月、9個月在平臥頭低位下行消化道X線鋇餐檢查，并長期隨訪，以評價手術效果。 結果本組均順利完成手術，無手術死亡，患者術前存在的食管下括約肌松弛和胃酸反流，術后均明顯改善。術后平均住院時間9（5～11）d。52例患者術后2周內復查上消化道X線鋇餐檢查，無胃食管反流。所有患者均隨訪，隨訪時間2個月至10年。除1例術后半年并發食管狹窄外，其余患者均恢復順利，癥狀消失，營養及發育好轉。隨訪期間無復發。 結論改良Nissen手術是治療滑動性食管裂孔疝的有效方法，加強食管下段高壓區，折疊胃及賁門口置于膈肌下方，不僅能使胃還納腹腔，同時還有抗反流的效果。

The First 10 Balloon Dilated Aortic Valve Replacement Patients in China

ObjectiveTo discuss the feasibility about the using of transcatheter aortic valve implantation (TAVI) in severe aortic valve stenosis chinese patients, then to make more exploration and accumulate enough experience. MethodsWe selected 10 out-patients with severe aortic valve stenosis and calcified with 9 males and 1 females at age of 76.4 (65-81) years in our hospital from January 2011 to March 2014. All the patients can't tolerate traditional open surgery through preoperative evaluation. So we chose the Sepian TX system, balloon dilated transcatheter aortic valve, to treat them via transfemoral approach. ResultsTen patients accomplished TAVI successfully. One patient was assisted by the left-ventricular puncture. No complication occurred. The function of aortic valve after TAVI improved significantly. The hospital stay time was 3-5 days. The patients were followed up for 3-34 months. One patient died of pulmonary cancer during the following-up. ConclusionTranscatheter balloon dilated aortic valve replacement can be used in chinese severe aortic valve stenosis patient, but more accurate preoperative preparation, evaluation, and operation are needed.