觀察受體鼠妊娠和胚胎著床情況,并檢測胚胎移植時小鼠子宮內膜中白血病抑制因子(Lif)表達水平,探討超排卵對小鼠胚胎著床潛能的影響。方法:建立超排周期胚胎和自然周期胚胎移植小鼠模型,比較妊娠率、胚胎著床率的差異及其與Lif蛋白的表達水平之間的關系。結果:超排卵周期受體組的妊娠率(20.00%)和胚胎著床率(8.33%)顯著低于自然周期組的妊娠率(55.00%)和胚胎著床率(35.00%)(P<0.05)。自然周期胚胎和超排周期胚胎受體組內膜中Lif蛋白的表達水平相似(P>0.05),妊娠受體組Lif蛋白的表達水平顯著高于未孕受體組(P<0.05),但單胎妊娠和多胎妊娠受體組內膜中Lif蛋白的表達水平相似(P>0.05)。結論:超排卵可能降低胚胎的著床潛能,Lif蛋白的表達水平與胚胎著床有關,但與著床胚胎的數目無比例關系。
【摘要】 目的 探討不同年齡段和不同內膜準備方法對凍融胚胎移植(FET)妊娠率的影響。 方法 回顧性分析2007年3月-2009年8月107例患者131個FET周期,比較不同年齡段、不同內膜準備方法的妊娠情況。 結果 ①lt;29歲、30~34歲、≥35歲患者的周期臨床妊娠率分別為45.28%、37.88%、33.33%,比較無統計學意義(Pgt;0.05)。②自然周期和激素替代周期內膜準備,兩種方法患者平均年齡、平均移植胚胎數、胚胎評分及胚胎細胞數比較無統計學意義(Pgt;0.05),但自然周期的胚胎種植率及周期臨床妊娠率均明顯低于激素替代周期(16.78%比29.21%;29.51%比50.00%),比較有統計學意義(Plt;0.05)。 結論 患者年齡不影響FET周期臨床妊娠率,激素替代周期內膜準備的FET胚胎種植率及周期臨床妊娠率明顯高于自然周期。【Abstract】 Objective To analyze the effect of age and method of preparing endometrium on the pregnancy rate of frozen-thawed embryo transfer (FET) among infertile women. Methods Retrospective analysis was made on 131 FET cycles in 107 patients from March 2007 to August 2009. The relationship between pregnancy rate of FET and following factors were analyzed: age, the method of preparing uterus endometrium. Results ①There was no significant difference in pregnancy rate of FET among <29, 30 - 34, and ≥35 years old patients (45.28%, 37.88%, and 33.33%, respectively) (Pgt;0.05). ②There was no significant difference in patients’ age, the average embryo amount, embryo score, the cell amount in every embryo between the FET with hormone replacement therapy (HRT) and the FET with natural cycle (NC). The embryo implantation rate and pregnancy rate of FET with NC were lower significantly than those of FET with HRT (16.78% vs 29.21%; 29.51% vs 50.00%) (P<0.05). Conclusion Age might not influence pregnancy rate of FET. FET with HRT has higher implantation rate and pregnancy rate than FET with NC.
摘要:目的: 探討二維及彩色多普勒超聲對妊娠合并卵巢扭轉的診斷價值。 方法 :對11例經手術及病理證實的妊娠合并單純卵巢扭轉,進行二維及彩色多普勒和能量多普勒超聲圖像分析。 結果 :11例患側卵巢在二維圖像中均有不同程度的腫大,超聲壓痛征均呈陽性反應;其中7例有明顯的位置改變,5例有不同程度的盆腔積液;彩色多普勒和能量多普勒顯示患側卵巢內部均無明顯血流信號。 結論 :妊娠期卵巢扭轉有典型超聲圖象特征,可為臨床提供較可靠的診斷依據。Abstract: Objective: To evaluate the diagnostic value of two dimension ultrasound (2DUS), color Doppler flow imaging (CDFI) and power Doppler imaging (PDI) for ovarian torsion during the pregnant period.〖WTHZ〗Methods : Using the techniques of 2DUS, CDFI and PDI to analyze sonographic features of 11 ovarian torsion cases which were dominated by operation and pathology.〖WTHZ〗Results : The tumescent sick ovaries were detected in all case with ovaries torsion by 2DUS. The patient felt pain when their sick ovaries were pressed. The position of seven sick ovaries was changed. The pelvic effusion was detected in five cases. The blood flow signal was not demonstrated in all sick ovaries by CDFI and DPI.〖WTHZ〗Conclusion : The ultrasonic imaging characteristics are reliable to diagnose ovarian torsion during the pregnant period.
ObjectivesTo systematically review the efficacy and safety of local endometrial mechanical stimulation in patients with recurrent embryo implantation failure in vitro fertilization.MethodsPubMed, EMbase, The Cochrane Library, CNKI, WanFang Data and VIP databases were electronically searched to collect clinical controlled studies on the efficacy and safety of local endometrial mechanical stimulation in patients with recurrent embryo implantation failure from inception to March 2018. Two reviewers independently screened literature, extracted data and assessed the risk of bias of included studies, then, meta-analysis was performed by using RevMan 5.3 software.ResultsA total of 10 studies, including 8 randomized controlled trials and 2 case-control studies, and involving 1 274 patients were included. The results of meta-analysis showed that: the clinical pregnancy rate of endometrial mechanical stimulation group was higher than that of control group (RR=1.40, 95%CI 1.04 to 1.89, P=0.03). However, no significant differences were found in implantation rate (RR=0.75, 95%CI 0.50 to 1.13, P=0.17), live births rate (RR=1.38, 95%CI 0.99 to 1.93, P=0.06), miscarriage rate (RR=0.83, 95%CI 0.55 to 1.24, P=0.36) and rate of multiple pregnancy (RR=0.90, 95%CI 0.61 to 1.35, P=0.63).ConclusionCurrent evidence shows that, for patients with repeated implantation failure, mechanical endometrial stimulation before re-transplantation may help to improve the clinical pregnancy rate of test-tube infants, however, it has no significant effects on implantation rate, live birth rate, abortion rate, multiple pregnancy rate and ectopic pregnancy rate. Due to limited quality and quantity of the included studies, more high quality studies are needed required to verify above conclusions.