目的:探討肝脾γδT細胞淋巴瘤的臨床表現、病理學特征、免疫表型特點。方法:對我院2例確診的肝脾γδT細胞淋巴瘤患者的臨床資料進行分析、追蹤隨訪并進行文獻復習。 結果:該組患者均為青年男性,肝脾不同程度長大,發熱,全血細胞減少(1/2),肝功能受損,淋巴結未受累;病理示瘤細胞彌漫性肝、骨髓的竇內侵犯;免疫表型:患者瘤細胞表達CD2(+)、CD3(+)、CD56(+)、CD16(+)、CD20()、TIA1(+)、TCRγ／δ(+)。結論:肝脾γδT細胞淋巴瘤是較為罕見的外周T細胞淋巴瘤,以肝脾長大、發熱為主要臨床表現,通常淋巴結不受累,病情進展快,療效差,生存期短。
目的 探討不同分子分型乳腺浸潤性導管癌手術病例標本中P53、表皮生長因子受體（EGFR）和Ki-67的表達及臨床意義。 方法 采用免疫組織化學鏈霉菌抗生物素蛋白-過氧化物酶連接法法對2010年1月－2011年7月446例乳腺浸潤性導管癌患者標本進行分子分型，并同時檢測其P53、EGFR、Ki-67等的表達。 結果 P53和Ki-67在人類表皮生長因子受體2（HER2）過表達型、基底細胞樣型、未分類型中的表達明顯強于管腔A型及管腔B型（P＜0.05）；HER2過表達型和未分類型中的EGFR表達明顯強于管腔A型及管腔B型（P＜0.05）。 結論 在使用雌激素受體、c-erbB-2等指標對浸潤性導管癌進行分子分型時同時檢測P53、EGFR及Ki-67等標記物，有助于更加精準的評估腫瘤的生物學行為及預后 ，對靶向藥物的個體化治療提供參考和療效預測有重要意義。
Objective To investigate the value of a 4-color and 10-antibody flow cytometry immunophenotyping panel using 10 antibodies including CD45, CD38, CD19, CD56, CD20, CD5, CD10, human leukocyte antigen-DR (HLA-DR), κ antibody and λ antibody marked by four kinds of fluorescein including R-phycoerythrin (PE), fluorescein isothiocyanate (FITC), peridinin chlorophy Ⅱ protein (PerCP) and allophycocyanin (APC) in the diagnosis of multiple myeloma (MM). Methods A 4-color and 10-antibody flow cytometry immunophenotyping panel which used CD45dim/-/CD38high as gating strategy supplemented by CD19, CD56, CD20, CD10, CD5, HLA-DR, κ antibody and λ antibody was used to test the bone marrow (BM) specimens of 45 MM patients treated between December 2013 and March 2015. Then by morphological examination, we analyzed the quantitative results and characteristics of myeloma cells. Results In all the 45 MM patients, the myeloma cell detection rate was 100% by flow cytometry. The proportion range of myeloma cells in BM was between 1.17% and 72.31%, which showed a good consistency with the results of 7.5%-90.0% detected by morphological examination. The positive expression rates of antigen on myeloma cells were: 100.00% for CD38, 11.11% for CD45, 2.22% for CD19, 73.33% for CD56, 17.78% for CD20, 42.22% for HLA-DR, and 0% for CD10 and CD5. About 64.44% of the MM patients were restricted cytoplasmic λ light chain typing, and 35.56% were restricted cytoplasmic κ light chain typing. There was no obvious phenotype difference among the 3 Durie-Salmon stages of MM (P>0.05). The expression of CD56 was different among different immunoglobulin types of MM, and the types of immunoglobulin with an expression from high to low were non-secretory, IgA, IgG, and light chain (P<0.05). Conclusion The 4-color and 10-antibody flow cytometry immunophenotyping panel using 10 antibodies including CD45, CD38, CD19, CD56, CD20, CD5, CD10, HLA-DR, κ antibody and λ antibody marked by four kinds of fluorescein including PE, FITC, PerCP and APC has a good diagnostic value for MM.
ObjectiveTo study the clinicopathological features of mediastinum nodular sclerosis Hodgkin lymphoma (NSHL) in order to improve the recognition of it. MethodsThe clinical data of 3 cases of mediastinum NSHL between 2003 and 2012 were collected. Then we analyzed the carcinoma pathologic samples by pathomorphology, immunophenotypic phenotype, related gene rearrangement and situ hybridization with EBER. ResultsThe pathomorphologic results showed that broad fibrotic bands subdivided the lymphoid parenchyma into large nodules, the tumoral cells had distinct boundary with empty cytoplasm and small-to-medium-sized nucleoli, and the nodules contained inflammatory cell components. The immunophenotypic phenotype of the tumoral cells were CD15, CD30, PAX-5 and CD20 partly, but anaplastic lymphoma kinase, CD45, cytokeratin, CD79α and S-100 were not expressed. T cell receptor γ and IgH gene were no rearranged, and EBER in situ hybridization was not detected. ConclusionVarious lymphomas occur in the mediastinum and mediastinum NSHL is just one of them. Mastering its distinctive pathomorphology and immunophenotypic phenotype is highly significant for diagnosis, differential diagnosis and treatment of the disease.
目的 建立急性白血病（AL）患者八色流式免疫表型分析起始管方案。 方法 用胞膜CD3（CD3）、CD19、CD10、CD34、CD45、胞漿CD79a（cCD79a）、髓過氧化物酶（MPO)和胞漿CD3（cCD3）等8種抗體建立八色流式染色方案。膜表面抗體直接染色；膜內抗體經固定破膜，再染色后上機檢測。將3個血小板減少患者骨髓標本分別進行抗體的單色染色和缺一色染色；最后對17例確診的AL初發患者標本進行檢測。 結果 用單色染色來確定染色方案中各抗體的檢測電壓及熒光補償；缺一色染色中，陽性細胞群較單色染色變化均＜10%，表明方案中的各抗體相互作用小。17例AL初發患者中，6例急性B淋巴細胞白血病原始細胞均為CD34和CD19陽性，5例cCD79a陽性和4例CD10陽性；4例急性T淋巴細胞白血病患者均為cCD3陽性；6例急性髓細胞白血病均為CD34和MPO陽性；1例B+T混合表型AL患者CD34、cCD3、CD19、cCD79a及CD10均為陽性，MPO和CD3為陰性，此檢測方案能夠確定各類AL的細胞類型。 結論 建立了AL患者八色流式免疫表型分析起始管方案，操作簡便快速，適用于臨床檢測。