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Vitamin C Promoting Embryonic Stem Cells Co-cultured on Poly 3-Hydroxybutyrate-co-4-Hydroxybutyrate to Differentiating into Myocardiocytes

ObjectiveTo assess the suitability of P (3HB-co-4HB) combined with embryonic stem cells (ESCs) for myocardial patch formation and whether adding vitamin C would improve inductivity or not. Method We extracted mouse embryonic fibrous cell from three clean female white Kunming mouses at a mean body weight of 37.5 grams. We recovered and cultured mouse ESCs. Those mouse embryonic stem cells were obtained from Shanghai Institutes of Biological Sciences. We took pendant-drop method to form embryonic bodies (EBs) and co-cultured them with myocardial patch. The experimental group were cultured in the substate with vitamin C while the control group were cultured in the substate without vitamin C. We immunostained the myocardial patch and observed them by scanning electron microscope. We calculated the differentiation efficiency and mapped the distribution curve of induction time. ResultsThe scattergram showed that the differentiation efficiency increased gradually. The differentiation efficiency of the group with vitamin C was 71.1% and the group without vitamin C was 17.8%. There was a statistical difference between the two groups (P < 0.05). ConclusionOn the biological patch of P (3HB-co-4HB), ESCs could grow, proliferate, and differentiate into myocardial cell and adding vitamin C into it could improve the differentiation efficiency.

體外循環與非體外循環冠狀動脈旁路移植術臨床分析

目的 比較體外循環冠狀動脈旁路移植術（on—pump CABG，on—pump）和非體外循環冠狀動脈旁路移植術（off—pump CABG，off—pump）的臨床應用效果。方法 回顧2003年12月-2005年1月間我院行冠狀動脈旁路移植術（CABG）195例的臨床資料，依據術中采用不同的手術方式分為on—pump組和off—pump組。并對兩組患者的術前、術后臨床資料、術后并發癥發生情況進行分析。結果 兩組患者術前一般臨床資料比較差別無統計學意義；術后off—pump組發生并發癥17例，on—pump組26例（P=0．000）；off-pump組住院死亡2例，on—pump組死亡6例（P=0．025）。結論 在多支左主干病變中，off—pump和on—pump在再血管化的應用上無差別；off—pump后的心電圖改變、死亡率和并發癥發生率比on—pump患者低，但是合并瓣膜病或嚴重的3支血管病變、左主干病變、室壁瘤形成的患者更適宜采用on—pump，off—pump不能取代on—pump。

主動脈瓣下狹窄老年患者一例

Hypoxic three-dimensional culture microenvironment promotes proliferation of bone marrow mesenchymal stem cells through HIF-1α signaling pathway

ObjectiveTo investigate the effects of hypoxic three-dimensional culture microenvironment on the proliferation of bone marrow mesenchymal stem cells and its mechanism. MethodsP5 generation mouse bone marrow mesenchymal stem cells and P (3HB-co-4HB) were co-cultured under normoxic three-dimensional (20%) and hypoxic three-dimensional microenvironment (4%) respectively. After 24 hours, the proliferation of the two groups was determined by CCK-8 method. The expression of HIF-1α gene was detected by real-time quantitative PCR after 12 hours. Western blotting was used to detect the expression of HIF-1α protein after 24 hours. ResultsAfter 24 hours, the CCK-8 method showed that the OD value of the hypoxia group was significantly higher than that of the normoxia group (0.455±0.027 vs. 0.352±0.090, n=12, P<0.05). After 12 hours of hypoxic culture, the expression level of HIF-1α mRNA in the hypoxia group was significantly higher than that in the normoxia group (P<0.05). Compared with the normoxia group (0.47± 0.05), the relative expression level of HIF-1α protein in the hypoxia group (0.63±0.06) significantly increased in the Western blotting after 24 hours (n=3, P<0.05). ConclusionThe hypoxic three-dimensional microenvironment can promote the proliferation of bone marrow mesenchymal stem cells, which may be related to the activation of HIF-1α signaling pathway.

延伸入右心房內腫瘤的外科治療

目的　總結膈下腫瘤蔓延至右心房的外科治療經驗。　方法　自 1991年 5月～ 2 0 0 2年 11月外科治療膈下腫瘤蔓延至右心房 6例 ,病變來源于子宮平滑肌瘤 3例 ,子宮間質肉瘤 1例 ,下腔靜脈粘液肉瘤 1例 ,腎平滑肌肉瘤 1例。 5例完整切除右心房和下腔靜脈內腫瘤 ,1例部分切除。　結果　無手術死亡 ,1例粘液肉瘤患者部分切除腫瘤術后 5個月死亡 ,其余患者隨診 5個月～ 3年 ,下腔靜脈內無腫瘤復發 ,無明顯癥狀。　結論　膈下腫瘤蔓延至右心房應積極手術治療 ,手術盡量完整切除腫瘤 ,中期療效良好。

冠狀動脈CT血管造影診斷冠狀動脈左前降支左心室瘺一例

Experimental Research of Small Molecule Compound XAV939 Inducing Mouse Embryonic Stem Cell into Cardiac Myocyte

ObjectiveTo investigate the feasibility of small molecule compound XAV939 to induce mouse embryonic stem cells (mESC) to differentiate into cardiac myocytes. MethodsWe revived and cultured undifferentiated mESC growing confluently on trophoderm made of mouse embryonic inoblast cell. The mESCs were digested by trypsin to form embryoid bodies (EBs) by handing drop method. After plated, EBs were induced by XAV939 to differentiate into cardiac myocytes. We observed the cardiac myocytes with lightmicroscopy and identified it with immunofluorescence method. Result The XAV939 can effectively induce mESC into cardiac myocytes with the mean efficiency rate of 71.85%±1.05%. The differentiated cardiac myocytes shrinked spanteously and rhythmicly. The cardiac troponin T as the special marker of cardiac myocyte was positive. ConclusionThe small molecule compound XAV939 could effectively induce mES cells into cardiac myocytes.

二尖瓣置換術后三尖瓣關閉不全的外科治療

目的總結二尖瓣置換術后三尖瓣關閉不全患者的外科治療經驗。方法14例心臟瓣膜疾病患者行二尖瓣置換術后發生中度或重度三尖瓣關閉不全,其中相對性關閉不全12例,器質性關閉不全2例。行三尖瓣成形術4例,均使用人工瓣環成形;行三尖瓣置換術10例,其中置換生物瓣6例,雙葉機械瓣4例。在心臟不停跳下手術4例,常規心臟停搏手術10例。結果全組無手術死亡,術后發生低心排血量綜合征3例,用升壓藥維持循環,患者均順利出院。術后復查超聲心動圖,右心房、室均明顯縮小,右心室前后徑(22.5±5.6mm)較術前(31.9±5.9mm)明顯減小(Plt;0.001)。隨訪14例,隨訪時間1.8±1.3年,心功能級2例、級9例,級2例,1例于術后4年因心力衰竭死亡。結論二尖瓣置換術后遠期三尖瓣關閉不全的患者經外科矯治后療效滿意,合理掌握手術指征、手術時機和良好的圍術期治療是手術成功的關鍵。

Experimental Study on PHBHHx Co-culturing with Mouse Induced Pluripotent Stem Cells outside Body

ObjectiveTo study the external biocompatibility bewteen the mouse induced pluripotent stem cells (miPSCs) and poly-3-hydroxybutyrate-co-3-hydroxyhexanoate (PHBHHx). MethodsAfter we recovered and subcultured miPSCs, we divided them into two groups. There was one group cultured with material of PHBHHx films outside the body. We observed the adhesive pattern of miPSCs on film by fluorescence of 4, 6-diamidino-2-phenylindole (DAPI) staining. The cell vitality was detected by cell counting kit-8 (CCK-8). The morphology of miPSCs attached on the film was visualized under scanning electron microscope (SEM). We used the traditional petri dish to culture miPSCs and detect the cell activity by CCK-8. ResultsMiPSCs can adhere and proliferate on PHBHHx films. The result of cell vitality which detected by CCK-8 showed that there was a statistical difference in OD value between culturing on PHBHHx films and traditional cultivation (0.617±0.019 vs. 0.312±0.004, P < 0.05). ConclusionThere are adhesion and proliferation on the surface of cells patch made by miPSCs co-culturing with PHBHHx film. Compared with traditional culturing in the cell culture dish, culturing in PHBHHx films have great advantages in the process of adhesion and proliferation. PHBHHx can be used as one of the scaffold for stem cells treating various disease.

主動脈瓣四葉畸形合并腎移植術后行主動脈瓣置換術一例