Objective To investigate the mRNA and protein expression of human β-defensin-2 (hBD-2) induced by lipopolysaccharide (LPS)，IL-1β and TNF-α in human airway primary epitheliums.Methods The bronchial primary epitheliums from human were stimulated with LPS，IL-1β and TNF-α respectively and then were harvested for hBD-2 expression detection.The mRNA expression of hBD-2 was detected by RT-PCR，and the protein expression by immunocytochemistry and western blot.Results There was a small expression of hBD-2 mRNA in human airway primary epitheliums before stimulation.The hBD-2 mRNA expression was significantly increased after 3 hours of LPS，IL-1β and TNF-α stimulation respectively and the expression increasement was in a dose dependent manner.The hBD-2 protein could be detected in cytoplasm after 4 hours of LPS (0.1 μg/mL)，IL-1β (1 ng/mL) and TNF-α (10 ng/mL) stimulation.Conclusions LPS and proinflammatory cytokines can induce the mRNA and protein expression of hBD-2 in a short time.The expression of hBD-2 may play an initial defense role against bacterial invasion.
目的 研究CD14過表達對胃癌細胞核轉錄因子-κB（NF-κB）活性以及人β防御素-2（hBD-2）表達的影響，探討CD14在胃癌發生發展中的作用。 方法 體外培養CD14穩定轉染的胃癌SGC-7901細胞系及空質粒轉染的對照細胞，CD14蛋白受體胞壁酰二肽刺激細胞，凝膠遷移實驗檢測NF-κB的活性，蛋白質印跡法檢測NF-κB蛋白的表達，同時分別用逆轉錄-聚合酶鏈反應以及蛋白質印跡法檢測hBD-2 mRNA及蛋白的表達。 結果 與對照組相比，CD14過表達的細胞中NF-κB的活性明顯增強，蛋白表達量也大幅度增加，同時hBD-2的mRNA及蛋白的表達都有所提高。 結論 胃癌細胞中CD14在介導NF-κB的激活以及hBD-2的表達中發揮重要作用。