Quantitation of Minichromosome Maintenance 2 mRNA Expression in Carcinoma, Adenoma and Normal Mucosa of Colon with Real-Time RT-PCR Assay_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

1.Division of Digestive Surgery and Organ-Microcirculation;;
2.Ocular Surgery Laboratory;;
3. Department of Pathology;;
West China Hospital, Sichuan University, Chengdu 610041, ChinaCorresponding Author： ZHOU Zong-guang, E-mail： Zhou767@163.com;

Corresponding?author：

Keywords：

Colonic adenoma; Colonic carcinoma; Minichromosome maintenance protein 2; Real-time RT-PCR

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Objective 　 To investigate the difference of minichromosome maintenance protein 2 (MCM2) mRNA expression among colonic normal mucosa, colonic adenoma and carcinoma.
Methods 　The expressions of MCM2 mRNA were determined by real-time RT-PCR in 12 colonic normal mucosa, 33 colonic adenomas and 12 colonic carcinomas. Data were evaluated by relative expression software tool (REST-XL).
Results 　The expressions of MCM2 mRNA elevated in turn by colonic normal mucosa, adenoma and carcinoma. The expression of MCM2 mRNA in colonic carcinomas was significantly higher than that in adenomas， as well as in normal mucosa (P=0.001), while the difference of MCM2 mRNA expressions between adenoma and normal mucosa was insignificant (P=0.184).
Conclusion 　The difference of MCM2 mRNA expressions between adenoma and carcinoma indicated the potential value on early diagnosis for colonic carcinoma.

Citation： WANG Yong,ZHOU Zongguang,XIA Qingjie,ZHANG Wenyan,LI Hongguang,WANG Rong. Quantitation of Minichromosome Maintenance 2 mRNA Expression in Carcinoma, Adenoma and Normal Mucosa of Colon with Real-Time RT-PCR Assay. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2008, 15(8): 603-606. doi: Copy

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7.	Gonzalez MA, Pinder SE, Callagy G, et al. Minichromosome maintenance protein 2 is a strong independent prognostic marker in breast cancer [Ｊ]. J Clin Oncol, 2003; 21(23)∶ 4306.
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12.	Murakami R, Tsukuma H, Kanamori S, et al. Natural history of colorectal polyps and the effect of polypectomy on occurrence of subsequent cancer [Ｊ]. Int J Cancer, 1990; 46(2)∶ 159.
13.	Rocha Ramirez JL, Pea JP, Franco Gutierrez JR, et al. Colonic adenoma: risk factors for their malignant transformation [Ｊ]. Rev Gastroenterol Mex, 1996; 61(3)∶ 178.
14.	Yang L, Zhou ZG, Lou HZ, et al. Quantitative analysis of PPAR delta mRNA by real-time RT-PCR in 86 rectal cancer tissues [Ｊ]. Eur J Surg Oncol, 2006; 32(2)∶ 181.
15.	王永，周總光，李紅光. 利用MCM2檢測細胞的增殖性在大腸腫瘤診斷中的應用 [Ｊ]. 中國普外基礎與臨床雜志， 2006； 13(1)∶ 123.
16.	王小林，卿三華，黃祥成，等. RT-PCR檢測直腸癌在直腸系膜的播散范圍及臨床意義 [Ｊ]. 中國普外基礎與臨床雜志， 2005； 12(1)∶ 29.
17.	Freeman A, Morris LS, Mills AD, et al. Minichromosome maintenance proteins as biological markers of dysplasia and malignancy [Ｊ]. Clin Cancer Res, 1999; 5(8)∶ 2121.
18.	Hunt DP, Freeman A, Morris LS, et al. Early recurrence of benign meningioma correlates with expression of mini- chromosome maintenance 2 protein [Ｊ]. Br J Neurosurg, 2002; 16(3)∶ 10.
19.	Rodins K, Cheale M, Coleman N, et al. Minichromosome maintenance protein 2 expression in normal kidney and renal cell carcinomas： relationship to tumor dormancy and potential clinical utility [Ｊ]. Clin Cancer Res, 2002; 8(4)∶ 1075.
20.	Davies RJ, Freeman A, Morris LS, et al. Analysis of minichromosome maintenance proteins as a novel method for detection of colorectal cancer in stool [Ｊ]. Lancet, 2002; 359(9321)∶ 1917.
21.	楊剛. 英格蘭和北愛爾蘭2002年和2003年結直腸外科年會紀要 [Ｊ]. 中華胃腸外科雜志, 2004； 7(2)∶ 155.

1. 　Markowitz AJ, Winawer SJ. Management of colorectal polyps [Ｊ]. CA Cancer J Clin, 1997; 47(2)∶93.
2. 　莫善兢，金凡，孫璐. 上海大腸癌發病情況變化的研究 [Ｊ]. 上海預防醫學雜志， 1998； 10(1)∶2.
3. 　Tan DF, Huberman JA, Hyland A, et al. MCM2—a promising marker for premalignant lesions of the lung： a cohort study [Ｊ]. BMC Cancer, 2001; 1∶ 6.
4. 　Todorov IT, Werness BA, Wang HQ, et al. HsMCM2/BM28： a novel proliferation marker for human tumors and normal tissues [Ｊ]. Lab Invest, 1998; 78(1)∶ 73.
5. 　Sirieix PS, O’Donovan M, Brown J, et al. Surface expression of minichromosome maintenance proteins provides a novel method for detecting patients at risk for developing adenocarcinoma in barretts esophagus [Ｊ]. Clin Cancer Res, 2003; 9(7)∶ 2560.
6. 　Todorov IT, Attaran A, Kearsey SE. BM28, a human member of the MCM2-3-5 family, is displaced from chromatin during DNA replication [Ｊ]. J Cell Biol, 1995; 129(6)∶ 1433.
7. 　Gonzalez MA, Pinder SE, Callagy G, et al. Minichromosome maintenance protein 2 is a strong independent prognostic marker in breast cancer [Ｊ]. J Clin Oncol, 2003; 21(23)∶ 4306.
8. 　Yang J, Ramnath N, Moysich KB, et al. Prognostic significance of MCM2, Ki-67 and gelsolin in non-small cell lung cancer [Ｊ]. BMC Cancer, 2006; 6∶ 203.
9. 　Dudderidge TJ, Stoeber K, Loddo M, et al. MCM2, Geminin, and Ki-67 define proliferative state and are prognostic markers in renal cell carcinoma [Ｊ]. Clin Cancer Res, 2005; 11(7)∶ 2510.
10. 　Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR [Ｊ]. Nucleic Acids Res, 2001; 29(9)∶ e45.
11. 　Pfaffl MW, Horgan GW, Dempfle L. Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR [Ｊ]. Nucleic Acids Res, 2002; 30(9)∶ e36.
12. 　Murakami R, Tsukuma H, Kanamori S, et al. Natural history of colorectal polyps and the effect of polypectomy on occurrence of subsequent cancer [Ｊ]. Int J Cancer, 1990; 46(2)∶ 159.
13. 　Rocha Ramirez JL, Pea JP, Franco Gutierrez JR, et al. Colonic adenoma: risk factors for their malignant transformation [Ｊ]. Rev Gastroenterol Mex, 1996; 61(3)∶ 178.
14. 　Yang L, Zhou ZG, Lou HZ, et al. Quantitative analysis of PPAR delta mRNA by real-time RT-PCR in 86 rectal cancer tissues [Ｊ]. Eur J Surg Oncol, 2006; 32(2)∶ 181.
15. 　王永，周總光，李紅光. 利用MCM2檢測細胞的增殖性在大腸腫瘤診斷中的應用 [Ｊ]. 中國普外基礎與臨床雜志， 2006； 13(1)∶ 123.
16. 　王小林，卿三華，黃祥成，等. RT-PCR檢測直腸癌在直腸系膜的播散范圍及臨床意義 [Ｊ]. 中國普外基礎與臨床雜志， 2005； 12(1)∶ 29.
17. 　Freeman A, Morris LS, Mills AD, et al. Minichromosome maintenance proteins as biological markers of dysplasia and malignancy [Ｊ]. Clin Cancer Res, 1999; 5(8)∶ 2121.
18. 　Hunt DP, Freeman A, Morris LS, et al. Early recurrence of benign meningioma correlates with expression of mini- chromosome maintenance 2 protein [Ｊ]. Br J Neurosurg, 2002; 16(3)∶ 10.
19. 　Rodins K, Cheale M, Coleman N, et al. Minichromosome maintenance protein 2 expression in normal kidney and renal cell carcinomas： relationship to tumor dormancy and potential clinical utility [Ｊ]. Clin Cancer Res, 2002; 8(4)∶ 1075.
20. 　Davies RJ, Freeman A, Morris LS, et al. Analysis of minichromosome maintenance proteins as a novel method for detection of colorectal cancer in stool [Ｊ]. Lancet, 2002; 359(9321)∶ 1917.
21. 　楊剛. 英格蘭和北愛爾蘭2002年和2003年結直腸外科年會紀要 [Ｊ]. 中華胃腸外科雜志, 2004； 7(2)∶ 155.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Quantitation of Minichromosome Maintenance 2 mRNA Expression in Carcinoma, Adenoma and Normal Mucosa of Colon with Real-Time RT-PCR Assay

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