Long-Term Primary Culture of Human Papillary Thyroid Carcinoma Cells_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

BIAN Xuehai ¹ , ZHAO Jisheng ² , ZHANG Xiuying ³ , ZHANG Chunhai ¹ , FU Yantao ¹ , ZHAO Lina ¹ , SUN Hui ¹ , ZHENG Zelin. ²

1.Department of Thyroid Surgery， ChinaJapan Union Hospital of Jilin University, Changchun 130033， Jilin Province, China;;
2.Department of Gastrointestinal Surgery， ChinaJapan Union Hospital of Jilin University, Changchun 130033， Jilin Province, China;;
3. School of Nursing of Jilin University, Changchun 130033， Jilin Province, China;

Corresponding?author：

Keywords：

Papillary thyroid carcinoma; Primary culture; Thyroglobulin; Thyroid peroxidase; Immunohistochemistry

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ObjectiveTo investigate the primary culture method of human papillary thyroid carcinoma (PTC) cells for a long term and establish a monitoring and verification measures. MethodsPTC cells were isolated following routine procedures and cultured in the DMEM supplemented with 10% fetal bovine serum, glutamine, and 20 ng/ml epidermal growth factor (EGF). Thyroglobulin (Tg) and thyroperoxidase (TPO) in nutrient solution and specific antigen Tg expression of PTC cells cultured for different days were observed. ResultsThe PTC cells grew satisfactorily up to 45 days of incubation. Tg content in nutrient solution expressed the training period of a linear singular parabolic, achieved peak value (985.2 μg/L) at about 14 d. TPO had not been detected in nutrient solution. The Tg expressed positively by immunization fluorescent dyeing. ConclusionsPTC cells cultured in the present method can survive to over 45 days. A brief monitoring and evaluation systems of PTC cells has been established. This report prompts that cultured cells within 14 days maybe more suitable to gene research and provide alternative to the basic research of PTC events and features.

Citation： BIAN Xuehai,ZHAO Jisheng,ZHANG Xiuying,ZHANG Chunhai,FU Yantao,ZHAO Lina,SUN Hui,ZHENG Zelin.. Long-Term Primary Culture of Human Papillary Thyroid Carcinoma Cells. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2011, 18(8): 840-843. doi: Copy

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3.	邊學海，張廣，張純海，等．甲狀腺乳頭狀癌組織中Shh和Gli1的表達及其臨床意義研究 [J]．國際外科學雜志， 2011， 38（2）： 7881．.
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6.	任立軍，楊延芳，張成雷．甲狀腺乳頭狀癌淋巴結轉移規律的臨床研究 [J]．中國普外基礎與臨床雜志， 2011， 18（4）： 419422．.
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8.	Mazzaferri EL, Jhiang SM. Longterm impact of initial surgical and medical therapy on papillary and follicular thyroid cancer [J]. Am J Med, 1994, 97(5): 418428.
9.	吳立剛，劉奇倫，胡志強． MMP2和VEGFC在甲狀腺乳頭狀癌中的表達及臨床意義 [J]．中國普外基礎與臨床雜志， 2009， 16（11）： 906910．.
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12.	Jeker LT, Mehrdad H, Lynne BC, et al. Mouse thyroid primary culture [J]. Biochem Biophys Res Commun, 1999, 257(2): 511515.
13.	Antonelli A, Ferrari SM, Fallahi P, et al. Primary cell cultures from anaplastic thyroid cancer obtained by fineneedle aspiration used for chemosensitivity tests [J]. Clin Endocrinol (Oxf), 2008, 69(1): 148152.
14.	劉坤，羅軍，婁繁，等．大鼠骨髓基質細胞的體外培養和鑒定 [J]．中國普外基礎與臨床雜志， 2009， 16（11）： 875879．.
15.	Di FM, Coleoni AH, Pellizas CG, et al. Triiodothyronine induces proliferation in cultured bovine thyroid cells: evidence for the involvement of epidermal growth factorassociated tyrosine kinase activity [J]. J Endocrinol, 2000, 166(1): 173182.
16.	黃穎泓，楊立勇．人甲狀腺細胞體外培養的研究進展 [J]．醫學綜述， 2009， 15（10）： 14551458．.
17.	Visser TJ. The elemental importance of sufficient iodine intake: a trace is not enough [J]. Endocrinology, 2006, 147(5): 20952097.
18.	Mazzaferri EL, Robbins RJ, Spencer CA, et al. A consensus report of the role of serum thyroglobulin as a monitoring method for lowrisk patients with papillary thyroid carcinoma [J]. J Clin Endocrinol Metab, 2003, 88(4): 14331441.
19.	Miyauchi A, Kudo T, Miya A, et al. Prognostic impact of serum thyroglobulin doublingtime under thyrotropin suppression in patients with papillary thyroid carcinoma who underwent total thyroidectomy [J]. Thyroid, 2011, 21(7): 707716.
20.	陳佳瑞，王家東. 橋本甲狀腺炎合并甲狀腺乳頭狀癌外科診治(附51例臨床分析) [J]. 臨床耳鼻咽喉頭頸外科雜志， 2010, 24(6): 257260.
21.	Hartl DM, Al GA, Chami L, et al. High rate of multifocality and occult lymph node metastases in papillary thyroid carcinoma arising in thyroglossal duct cysts [J]. Ann Surg Oncol, 2009, 16(9): 25952601.
22.	莫祥蘭，黃振錄，周祥禎，等．高分子量角蛋白和TPO在甲狀腺乳頭狀癌診斷中的價值 [J]．中國實驗診斷學， 2006， 10（6）： 585587．.
23.	Müller K, Krohn K, Eszlinger M, et al. Effect of iodine on early stage thyroid autonomy [J]. Genomics, 2011, 97(2): 94100.
24.	Faggiano A, Severino R, Ramundo V, et al. Thyroid function in fabry disease before and after enzyme replacement therapy [J]. Minerva Endocrinol, 2011, 36(1): 15.
25.	Ott J, Promberger R, Kober F, et al. Hashimoto’s thyroiditis affects symptom load and quality of life unrelated to hypothyroidism: a prospective casecontrol study in women undergoing thyroidectomy for benign goiter [J]. Thyroid, 2011, 21(2): 161167.

1. Woodruff SL, Arowolo OA, Akute OO, et al. Global variation in the pattern of differentiated thyroid cancer [J]. Am J Surg, 2010, 200(4): 462466.
2. Pedersen IB, Laurberg P, Knudsen N, et al. An increased incidence of overt hypothyroidism after iodine fortification of salt in denmark:a prospective population study [J]. J Clin Endocrinol Metab, 2007, 92(8): 31223127.
3. 邊學海，張廣，張純海，等．甲狀腺乳頭狀癌組織中Shh和Gli1的表達及其臨床意義研究 [J]．國際外科學雜志， 2011， 38（2）： 7881．.
4. Delellis RA. Pathology and genetics of thyroid carcinoma [J]. J Surg Oncol, 2006, 94(8): 662669.
5. Troviseo V, Soares P, Preto A, et al. Molecular genetics of papillary thyroid carcinoma: great expectations [J]. Arq Bras Endocrinol Metabol, 2007, 51(5): 643653.
6. 任立軍，楊延芳，張成雷．甲狀腺乳頭狀癌淋巴結轉移規律的臨床研究 [J]．中國普外基礎與臨床雜志， 2011， 18（4）： 419422．.
7. 于金明，徐忠法．腫瘤臨床通鑒 [M]．濟南：山東科學技術出版社， 2004： 369375．.
8. Mazzaferri EL, Jhiang SM. Longterm impact of initial surgical and medical therapy on papillary and follicular thyroid cancer [J]. Am J Med, 1994, 97(5): 418428.
9. 吳立剛，劉奇倫，胡志強． MMP2和VEGFC在甲狀腺乳頭狀癌中的表達及臨床意義 [J]．中國普外基礎與臨床雜志， 2009， 16（11）： 906910．.
10. 袁振芳，高研，王薇，等． γ干擾素對培養的人甲狀腺細胞HLADR抗原基因表達的影響 [J]．中華內科雜志， 2002， 41（4）： 262263．.
11. 劉東方，鄧華聰，許改平，等．人甲狀腺原代細胞的長期培養 [J]．中華內分泌代謝雜志， 2005， 21（2）： 181183．.
12. Jeker LT, Mehrdad H, Lynne BC, et al. Mouse thyroid primary culture [J]. Biochem Biophys Res Commun, 1999, 257(2): 511515.
13. Antonelli A, Ferrari SM, Fallahi P, et al. Primary cell cultures from anaplastic thyroid cancer obtained by fineneedle aspiration used for chemosensitivity tests [J]. Clin Endocrinol (Oxf), 2008, 69(1): 148152.
14. 劉坤，羅軍，婁繁，等．大鼠骨髓基質細胞的體外培養和鑒定 [J]．中國普外基礎與臨床雜志， 2009， 16（11）： 875879．.
15. Di FM, Coleoni AH, Pellizas CG, et al. Triiodothyronine induces proliferation in cultured bovine thyroid cells: evidence for the involvement of epidermal growth factorassociated tyrosine kinase activity [J]. J Endocrinol, 2000, 166(1): 173182.
16. 黃穎泓，楊立勇．人甲狀腺細胞體外培養的研究進展 [J]．醫學綜述， 2009， 15（10）： 14551458．.
17. Visser TJ. The elemental importance of sufficient iodine intake: a trace is not enough [J]. Endocrinology, 2006, 147(5): 20952097.
18. Mazzaferri EL, Robbins RJ, Spencer CA, et al. A consensus report of the role of serum thyroglobulin as a monitoring method for lowrisk patients with papillary thyroid carcinoma [J]. J Clin Endocrinol Metab, 2003, 88(4): 14331441.
19. Miyauchi A, Kudo T, Miya A, et al. Prognostic impact of serum thyroglobulin doublingtime under thyrotropin suppression in patients with papillary thyroid carcinoma who underwent total thyroidectomy [J]. Thyroid, 2011, 21(7): 707716.
20. 陳佳瑞，王家東. 橋本甲狀腺炎合并甲狀腺乳頭狀癌外科診治(附51例臨床分析) [J]. 臨床耳鼻咽喉頭頸外科雜志， 2010, 24(6): 257260.
21. Hartl DM, Al GA, Chami L, et al. High rate of multifocality and occult lymph node metastases in papillary thyroid carcinoma arising in thyroglossal duct cysts [J]. Ann Surg Oncol, 2009, 16(9): 25952601.
22. 莫祥蘭，黃振錄，周祥禎，等．高分子量角蛋白和TPO在甲狀腺乳頭狀癌診斷中的價值 [J]．中國實驗診斷學， 2006， 10（6）： 585587．.
23. Müller K, Krohn K, Eszlinger M, et al. Effect of iodine on early stage thyroid autonomy [J]. Genomics, 2011, 97(2): 94100.
24. Faggiano A, Severino R, Ramundo V, et al. Thyroid function in fabry disease before and after enzyme replacement therapy [J]. Minerva Endocrinol, 2011, 36(1): 15.
25. Ott J, Promberger R, Kober F, et al. Hashimoto’s thyroiditis affects symptom load and quality of life unrelated to hypothyroidism: a prospective casecontrol study in women undergoing thyroidectomy for benign goiter [J]. Thyroid, 2011, 21(2): 161167.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Long-Term Primary Culture of Human Papillary Thyroid Carcinoma Cells

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