Clinical Practice of Detecting Cytokines in Pancreatitis Patients’ Peripheral Blood by Using Liquichip_West China Medical Journal

Authors：

MA Jian ¹ , LIU Rong ² , ZHANG Rui. ³

The Fifths People’s Hospital of Sichuan Province, Chengdu Sichuan 610031, China;

Corresponding?author：

ZHANG Rui., Email:

Keywords：

炎癥; 細胞因子; 液相芯片

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

目的:探討液相芯片檢測胰腺炎患者外周血中細胞因子濃度的可行性,及其在胰腺炎中的臨床應用價值。方法: 收集90例胰腺炎患者(包括重癥和輕癥)和30例正常對照者的外周血,離心提取血清,利用液相芯片檢測血清中細胞因子IL10,IL6,TNFα的濃度,比較不同程度炎癥患者以及與對照之間細胞因子的濃度差異,分析血清中細胞因子濃度差異與臨床表現之間的關系。結果:液相芯片可以方便地在僅25 μl的血清樣本中同時準確檢測這三種細胞因子的含量,檢測的有效范圍為0.10～2000 pg/mL。IL10 的濃度在對照、輕型和重型胰腺炎組中分別為51.97±31.72 pg/mL, 32.88±9.70 pg/mL和3.57±0.99 pg/mL, 輕型組與重型組間以及重型組和對照間差異有顯著性(P lt; 005)。IL10在發病三天內和三天后的濃度分別為 42.47±10.15 pg/mL和12.28±5.04 pg/mL, 兩者間差異有顯著性(P lt; 0.01). Il6 的濃度在三天后從60.90±24.37 pg/mL下降到34.52±13.57 pg/mL,但差異沒有顯著性(P gt;0.05);TNF-α的濃度在各組間沒有顯著差異(P gt;0.05)。結論:液相芯片檢測技術可以快速準確的檢測少量血樣標本中IL10,IL-6,TNFα等細胞因子的濃度,在胰腺炎患者中細胞因子IL-10的濃度與胰腺炎輕重程度以及患病時間密切相關,IL-10的濃度可以作為該病臨床處理的依據和藥物療效的監測指標。

Citation： MA Jian,LIU Rong,ZHANG Rui.. Clinical Practice of Detecting Cytokines in Pancreatitis Patients’ Peripheral Blood by Using Liquichip. West China Medical Journal, 2009, 24(8): 1943-1945. doi: Copy

1.	POORAN N, INDARAM A, SINGH P, et al. Cytokines (IL-6, IL-8, TNF): Early and Reliable Predictors of Severe Acute Pancreatitis[J]. Journal of Clinical Gastroenterology, 2003, 37(3): 263-266.
2.	JAFFE H A, BUHL R, MASTRANGELI A, et al. Crystal Organ specific cytokine therapy. Local activation of mononuclear phagocytes by delivery of an aerosol of recombinant interferongamma to the human lung[J]. J Clin Invest, 1991, 88(1): 297–302.
3.	TARANTINO G, COPPOLA A, CONCA P, et al. Serum TGFbeta 1 could be used to assess therapeutic outcome and shortterm prognosis of HCVrelated chronic hepatitis[J]. Int J Immunopathol Pharmacol, 2008, 21(4):1007-1012.
4.	OSMAN MO, GESSER B, MORTENSEN JT, et al. Profiles of proinflammatory cytokines in the serum of rabbits after experimentally induced acute pancreatitis[J]. Cytokine, 2002, 17(1):53-59.
5.	SALAMON M, SYSAJEDRZEJOWSKA A, LUKAMOWICZ J, et al. Concentration of selected cytokines in serum of patients with acne rosacea[J]. Przegl Lek, 2008, 65(9):371-374.
6.	SHARMA A, SATYAM A, SHARMA JB. Leptin, IL10 and inflammatory markers (TNFalpha, IL-6 and IL-8) in preeclamptic, normotensive pregnant and healthy nonpregnant women[J]. Am J Reprod Immunol, 2007, 58(1):21-30.

1. POORAN N, INDARAM A, SINGH P, et al. Cytokines (IL-6, IL-8, TNF): Early and Reliable Predictors of Severe Acute Pancreatitis[J]. Journal of Clinical Gastroenterology, 2003, 37(3): 263-266.
2. JAFFE H A, BUHL R, MASTRANGELI A, et al. Crystal Organ specific cytokine therapy. Local activation of mononuclear phagocytes by delivery of an aerosol of recombinant interferongamma to the human lung[J]. J Clin Invest, 1991, 88(1): 297–302.
3. TARANTINO G, COPPOLA A, CONCA P, et al. Serum TGFbeta 1 could be used to assess therapeutic outcome and shortterm prognosis of HCVrelated chronic hepatitis[J]. Int J Immunopathol Pharmacol, 2008, 21(4):1007-1012.
4. OSMAN MO, GESSER B, MORTENSEN JT, et al. Profiles of proinflammatory cytokines in the serum of rabbits after experimentally induced acute pancreatitis[J]. Cytokine, 2002, 17(1):53-59.
5. SALAMON M, SYSAJEDRZEJOWSKA A, LUKAMOWICZ J, et al. Concentration of selected cytokines in serum of patients with acne rosacea[J]. Przegl Lek, 2008, 65(9):371-374.
6. SHARMA A, SATYAM A, SHARMA JB. Leptin, IL10 and inflammatory markers (TNFalpha, IL-6 and IL-8) in preeclamptic, normotensive pregnant and healthy nonpregnant women[J]. Am J Reprod Immunol, 2007, 58(1):21-30.

West China Medical Journal

Clinical Practice of Detecting Cytokines in Pancreatitis Patients’ Peripheral Blood by Using Liquichip

Abstract Full text Figures/Tables Video References Cited by

Format

Content