Study on Bone Marrow Mesenchymal Stem Cells Differentiated into Hepatoid Cells in vitro_West China Medical Journal

West China Medical Journal

Supervisor：Ministry of Education of the People's Republic of China Sponsor：Sichuan University
Editor-in-chief：LUO Fengming
Publishing period： Monthly ISSN：1002-0179 CN：51-1356/R

Study on Bone Marrow Mesenchymal Stem Cells Differentiated into Hepatoid Cells in vitro

West China Medical Journal | 2010, 25(2): 310-
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Authors：

YANG Liu , LIU Hailin

The Department of Gastroenterology, The Ninth People’s Hospital, School of Medicine, Shanghai Jiaotong University;

Corresponding?author：

Keywords：

骨髓間充質干細胞; 肝樣細胞/肝細胞; 肝細胞生長因子; 成纖維細胞生長因子4

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【摘要】目的評價骨髓間充質干細胞(BMSCs)向肝樣細胞誘導的可行性。方法2008年1月2009年1月,以肝細胞生長因子(HGF) 20 ng/mL,成纖維細胞生長因子4(FGF4) 10 ng/mL為誘導劑,從細胞形態變化,并通過RTPCR、免疫組化方法分別對誘導第7、14、21及28天的細胞進行白蛋白(ALB)、甲胎蛋白(AFP)、細胞角蛋白18(CK18)等檢測。人L02肝細胞及未誘導的BMSCs分別為陽性和陰性對照結果BMSCs誘導7 d出現類圓形或多角形細胞,并出現鋪路石樣結構;誘導14 d細胞呈現典型的鋪路石狀;誘導21 d,同前;誘導28 d,細胞排列紊亂,局部細胞的形態不規則、細胞邊界不清。BMSCs誘導第7、14、21天ALB、CK18、AFP等mRNA表達陽性;未誘導BMSCs均為陰性;肝細胞ALB、CK18、AFP等mRNA表達均陽性。免疫細胞化學檢測結果同RTPCR。結論以HGF及FGF4為主的誘導體系可有效誘導BMSCs向肝樣細胞轉化,BMSCs可以作為一種新的肝細胞來源。

Citation： YANG Liu,LIU Hailin. Study on Bone Marrow Mesenchymal Stem Cells Differentiated into Hepatoid Cells in vitro. West China Medical Journal, 2010, 25(2): 310-. doi: Copy

West China Medical Journal

Study on Bone Marrow Mesenchymal Stem Cells Differentiated into Hepatoid Cells in vitro

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