• The Department of Oral and Maxillofacial Surgery, 2.Central Lab, Stomatology Hospital, Xi’an Jiaotong University;
RNA干擾; 涎腺黏液表皮樣癌; HER2基因
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【摘要】目的通過RNA干擾(RNAi)沉默HER2基因在涎腺黏液表皮樣癌Mc3細胞中的表達方法將目的基因靶序列小干擾RNA(siRNA)轉染Mc3細胞,并設置對照組,采用RTPCR、免疫組化檢測RNA干擾后HER2基因在Mc3細胞中的表達情況。結果RTPCR結果顯示RNA干擾后,HER2基因mRNA在涎腺黏液表皮樣癌細胞中的表達與對照組比較明顯降低;免疫組化實驗結果顯示RNA干擾后HER2基因蛋白在涎腺黏液表皮樣癌細胞中的表達降低,與mRNA表達情況相一致。結論RNA干擾成功抑制了涎腺黏液表皮樣癌細胞中HER2基因的表達,為口腔涎腺黏液表皮樣癌針對癌基因HER2為靶基因的基因治療提供研究基礎。

Citation： LIU Xiaohua,ZHANG Yincheng,REN Wenhao,CAO Tengteng,WEI Hong. RNA Interference Silence HER2 Expression in Salivary Gland Mucoepidermoid Carcinoma Cell Line Mc3. West China Medical Journal, 2010, 25(2): 306-. doi: Copy

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