The Influence of Fish Bile on the Renal Function of Rabbits and Preliminary Study on Its Mechanism_West China Medical Journal

Authors：

LUO Jingchao ¹ , REN Long ¹ , HAN Yuchen ¹ , Yan Qiao ¹ , MI Xuhua ² , MA Xingyi ² ,  YU Weiping ³

1. Medical School of Southeast University, Nanjing, Jiangsu 210009, P. R. China; 2. Department of Nephrology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China; 3. Department of Pathophysiology, Medical School of Southeast University, Nanjing, Jiangsu 210009, P. R. China;

Corresponding?author：

YU Weiping, Email: sucapter@163.com

Keywords：

魚膽汁; 急性腎損傷; 兔

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目的　探討魚膽汁對兔腎臟的影響及其機制。方法　將實驗新西蘭大耳白兔隨機分為灌胃組（GP組，n=19）與靜脈注射組（VI組，n=15），根據體重分別按3 mL/kg、0.3 mL/kg的劑量通過灌胃或耳緣靜脈注射方式給予魚膽汁。采集魚膽汁處理前與處理后1～5 h的血標本，測定腎功能、酸堿平衡及電解質指標，記錄GP組每個采樣點前20 min尿量及魚膽汁處理前、處理后5 h的尿常規。魚膽汁處理后5 h處死動物取腎做病理學檢查。結果　給予一定量魚膽汁后5 h內，兩組兔血肌酐（Scr）、尿素氮、K+呈升高趨勢（P均＜0.05），而血HCO3?濃度呈下降趨勢（P＜0.05），其中VI組兔Scr、血K+改變早于GP組。GP組記錄尿量明顯下降，尿pH值升高，蛋白定量試驗、隱血試驗結果均呈陽性。兩組兔腎組織病理檢查均顯示腎小球血管充盈，少量中性粒細胞浸潤；腎小管水腫及間質充血，部分有局灶性出血，腎間質損傷較腎小球更為嚴重。結論　無論經由消化道還是血管給予實驗兔魚膽汁均可導致急性腎功能損傷，與魚膽汁造成急性腎實質損傷、特別是腎小管間質損傷有關。

Citation： LUO Jingchao,REN Long,HAN Yuchen,Yan Qiao,MI Xuhua,MA Xingyi,YU Weiping. The Influence of Fish Bile on the Renal Function of Rabbits and Preliminary Study on Its Mechanism. West China Medical Journal, 2012, 27(6): 841-844. doi: Copy

1.	Deng Y, Xiao G, Jin Y, et al. Multiple organ dysfunction syndrome due to ingestion of fish gall [J]. Chin Med J (Engl), 2002, 115(7): 1020-1022.
2.	Patnaik R, Kar SS, Ray R, et al. Indian carp (Labeo rohita) gall bladder poisoning-report of four cases in a single family[J]. Indian J Pediatr, 2011, 78(6): 749-752.
3.	Kung SW, Chan YC, Tse ML, et al. Acute renal failure and hepatitis following ingestion of carp gallbladder[J]. Clin Toxicol (Phila) , 2008, 46(8): 753-757.
4.	Singh NS, Singh LK, Khaidem I, et al. Acute renal failure following consumption of raw fish gall-bladder from manipur[J]. J Assoc Physicians India, 2004, 52(9): 743-745.
5.	Xuan BH, Thi TX, Nguyen ST, et al. Ichthyotoxic ARF after fish gallbladder ingestion: a large case series from Vietnam[J]. Am J Kidney Dis, 2003, 41(1): 220-224.
6.	Yamamoto Y, Wakisaka O, Fujimoto S, et al. Acute renal failure caused by ingestion of the carp gall bladder： a report of 3 cases, with special reference to the reported cases in Japan[J]. Nihon Naika Gakkai Zasshi, 1988, 77(8): 1268-1273.
7.	Park SK, Kim DG, Kang SK, et al. Toxic acute renal failure and hepatitis after ingestion of raw carp bile[J]. Nephron, 1990, 56(2): 188-193.
8.	Centers for Disease Control and Prevention (CDC). Acute hepatitis and renal failure following ingestion of raw carp gallbladders--Maryland and Pennsylvania, 1991 and 1994[J]. MMWR Morb Mortal Wkly Rep, 1995, 44(30): 565-566.
9.	伍漢霖, 陳永豪, 莊棣, 等. 中國膽毒魚類的研究[J]. 上海水產大學學報, 2001, 10(2): 102-108.
10.	張磊, 張武臣. 魚膽中毒臨床診治研究進展[J]. 山東醫藥, 2010, 50(25): 113-114.
11.	孟新科, 鄧躍林. 魚膽中毒的研究進展[J]. 中國危重病急救醫學, 2000, 12(7): 437-438.
12.	Guyton AC, Hall JE. Textbook of medical physiology[M]. 11th ed. Philadelphia: Elsevier Inc, 2006: 412.
13.	石朝周, 毛宇峰, 樊啟新, 等. 膽毒性成份的研究[J]. 同濟醫科大學學報, 1992, 2l(3): 168-171.
14.	黃兆能. 魚膽中毒誘發G6PD缺乏癥急性溶血1例[J]. 臨床血液學雜志, 2000, 13(4) : 149.
15.	Baek JH, D’Agnillo F, Vallelian F, et al. Hemoglobin-driven pathophysiology is an in vivo consequence of the red blood cell storage lesion that can be attenuated in guinea pigs by haptoglobin therapy[J]. J Clin Invest, 2012, 122(4): 1444-1458.
16.	Gladwin MT, Kanias T, Kim-Shapiro DB. Hemolysis and cell-free hemoglobin drive an intrinsic mechanism for human disease[J]. J Clin Invest, 2012, 122(4): 1205-1208.

1. 　Deng Y, Xiao G, Jin Y, et al. Multiple organ dysfunction syndrome due to ingestion of fish gall [J]. Chin Med J (Engl), 2002, 115(7): 1020-1022.
2. 　Patnaik R, Kar SS, Ray R, et al. Indian carp (Labeo rohita) gall bladder poisoning-report of four cases in a single family[J]. Indian J Pediatr, 2011, 78(6): 749-752.
3. 　Kung SW, Chan YC, Tse ML, et al. Acute renal failure and hepatitis following ingestion of carp gallbladder[J]. Clin Toxicol (Phila) , 2008, 46(8): 753-757.
4. 　Singh NS, Singh LK, Khaidem I, et al. Acute renal failure following consumption of raw fish gall-bladder from manipur[J]. J Assoc Physicians India, 2004, 52(9): 743-745.
5. 　Xuan BH, Thi TX, Nguyen ST, et al. Ichthyotoxic ARF after fish gallbladder ingestion: a large case series from Vietnam[J]. Am J Kidney Dis, 2003, 41(1): 220-224.
6. 　Yamamoto Y, Wakisaka O, Fujimoto S, et al. Acute renal failure caused by ingestion of the carp gall bladder： a report of 3 cases, with special reference to the reported cases in Japan[J]. Nihon Naika Gakkai Zasshi, 1988, 77(8): 1268-1273.
7. 　Park SK, Kim DG, Kang SK, et al. Toxic acute renal failure and hepatitis after ingestion of raw carp bile[J]. Nephron, 1990, 56(2): 188-193.
8. 　Centers for Disease Control and Prevention (CDC). Acute hepatitis and renal failure following ingestion of raw carp gallbladders--Maryland and Pennsylvania, 1991 and 1994[J]. MMWR Morb Mortal Wkly Rep, 1995, 44(30): 565-566.
9. 　伍漢霖, 陳永豪, 莊棣, 等. 中國膽毒魚類的研究[J]. 上海水產大學學報, 2001, 10(2): 102-108.
10. 　張磊, 張武臣. 魚膽中毒臨床診治研究進展[J]. 山東醫藥, 2010, 50(25): 113-114.
11. 　孟新科, 鄧躍林. 魚膽中毒的研究進展[J]. 中國危重病急救醫學, 2000, 12(7): 437-438.
12. 　Guyton AC, Hall JE. Textbook of medical physiology[M]. 11th ed. Philadelphia: Elsevier Inc, 2006: 412.
13. 　石朝周, 毛宇峰, 樊啟新, 等. 膽毒性成份的研究[J]. 同濟醫科大學學報, 1992, 2l(3): 168-171.
14. 　黃兆能. 魚膽中毒誘發G6PD缺乏癥急性溶血1例[J]. 臨床血液學雜志, 2000, 13(4) : 149.
15. 　Baek JH, D’Agnillo F, Vallelian F, et al. Hemoglobin-driven pathophysiology is an in vivo consequence of the red blood cell storage lesion that can be attenuated in guinea pigs by haptoglobin therapy[J]. J Clin Invest, 2012, 122(4): 1444-1458.
16. 　Gladwin MT, Kanias T, Kim-Shapiro DB. Hemolysis and cell-free hemoglobin drive an intrinsic mechanism for human disease[J]. J Clin Invest, 2012, 122(4): 1205-1208.

West China Medical Journal

The Influence of Fish Bile on the Renal Function of Rabbits and Preliminary Study on Its Mechanism

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