Objective To investigate the antibiotic resistance and their genetic homology of stenotrophomonas maltophilia isolated from January 2005 to February 2006 at intensive care unit（ICU） of 6 hospitals in Bejing area.Methods The minimal inhibitory concentration（MIC） of 12 antibiotics against 82 strenotrophomonas maltophilia was determined by broth dilution method.PFGE was used to analyze the homology of 82 stenotrophomonas maltophilia.Results The drug sensitivity tests in vitro showed these strains were resistance to commonly-used antibiotics.Antibiotics with sensitive rate over 50% included Doxycycline, gatifloxacin,cefoperazone-sulbactam,levofloxacin,Compound sulfamethoxazole,Ceftazidime and ticarcillin- clavulanate. 7-18 DNA bands of different size were present in the gel and different homology was shown among the 82 strains.Four couples with homology over 85% were isolated from the same ICU.Three strain were same clones in PLA General Hospitals first hospital.2 couples from the different wards had homology of 80.6% and 79.6% of，respictively.Others strains had either poor or no homology.Conclusions No clonal outbreak is certified at ICU of 6 hospitals in Beijing area. There are only vertical dissemination of single clone in 6 ICU wards.PFGE is an effective approach for drug resistance test and epidemic analysis.
Citation：
DENG Xiaowei,LIU Changting,ZHENG Jingchen,LIANG Liwu,LI Yan,LI Hui,WANG Haiyan.. Drug resistance and its genetic homology of stenotrophomonas maltophilia at department of intensive care unit of 6 hospitals in Beijing. Chinese Journal of Respiratory and Critical Care Medicine, 2008, 08(1): 15-19. doi:
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Copyright ? the editorial department of Chinese Journal of Respiratory and Critical Care Medicine of West China Medical Publisher. All rights reserved
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- 1. 孫榮青，馮敏，徐珉，等.重癥監護病房革蘭陰性桿菌的感染分布與耐藥性監測.中國呼吸與危重監護雜志，2004，3：298-299.
- 2. 岳文香，黃紹光，聶森，等.重癥監護室嗜麥芽窄食單胞菌的分子流行病學研究.中華結核和呼吸雜志，2001，24：651-654.
- 3. 卓超，李小青.重癥監護病病房下呼吸道感染或定植嗜麥芽窄食單胞菌患者48例分析.中華燒傷雜志，2006，22：88-91.
- 4. Barbier-Frebour N，Boutiba-Boubake I，Nouvello M，et al.Molecular investigation of Stenotrophomonas maltophilia isolates exhibiting rapid emergence of ticarcillin-clavulanate resistance.J Hosp Infect，2000，45：35-41.
- 5. Chang LL，Chen HF，Chang CY，et al.Contribution of integrons，and SmeABC and SmeDEF efflux pumps to multidrug resistance in clinical isolates of Stenotrophomonas maltophilia.J Antimicrob Chemother，2004，53：518-521.
- 6. 卓超，錢元恕，李崇智，等.產金屬β-內酰胺酶嗜麥芽寡養單胞菌的研究.中國抗生素雜志，2003，28：753-756.
- 7. 孔暉，趙珊.嗜麥芽窄食單胞菌呼吸機相關性肺炎12例臨床分析.中國呼吸與危重監護雜志，2003，9：309.
- 8. 黎毅敏，黃紅川，劉曉青，等.重癥監護病房銅綠假單胞菌醫院獲得性感染暴發的流行病學調查.中國呼吸與危重監護雜志，2006，11：413-417.
- 9. Lavigne JP，Bouziges N，Chanal C，et al.Molecular epidemiology of Enterobacteriaceae isolates producing extended-spectrum beta-lactamases in a French hospital.J Clin Microbiol，2004，42：3805-3808.
