Effect and Mechanism of 17β-estradiol on Acrosome Reaction in Human Spermatozoa_West China Medical Journal

Authors：

1Department of Pathophysiology,Chengdu Medical College,Cheng du 610083; 2Reproductive Center of Mianyang Central Hospital,Mianyang 621000,China; 3Department of Physiology,West China School of Preclinical and Forensic Medicine,Sichuan University,Chengdu 61004,China; 4Department of Biochemistry,Mdical School of Qinghai University,Xining 810001,China;

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Keywords：

17β-雌二醇; 人精子; 頂體反應; 頂體酶; 鈣離子

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目的：探討雌激素影響人精子頂體反應的可能機制。方法：應用異硫氰酸熒光素標記豌豆凝集素熒光染色法（FITC-PSA）分析精子頂體反應（AR）、以分光光度比色法測定頂體酶（ACE）活性。結果：17β-雌二醇（17β-E2）可促進精子發生AR，并增強精子頂體酶的活性；去除培養液中的Ca2+后，17β-E2不能誘導精子發生AR；PKC抑制劑能明顯降低17β-E2所誘導的AR；E2-BSA亦能夠促進精子發生AR，其作用與17β-E2無顯著差異。結論：雌激素對人精子頂體反應有一定的促進作用，增強精子頂體酶活性可能是其作用途徑之一，此過程涉及了胞外Ca2+、PKC及精子膜上的ER或雌激素結合位點的參與。

Citation： HE Yanfang,ZHENG Jie,YUE Limin,et al.. Effect and Mechanism of 17β-estradiol on Acrosome Reaction in Human Spermatozoa. West China Medical Journal, 2008, 23(1): 34-35. doi: Copy

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1. Serge Carreau,Sonia Bourguiba,Sophie Lambard,et al.Reproductive system:aromatase and estrogens[J].Molecular and Cellular Endocrinology,2002,193(1-2):137-143.
2. World Health Organization.WHO laboratory manual for the exanlination of human sperm and sperm-cervical mucus interaction[M].4th ed.New York:Cambridge University Press,2001.
3. Revelli A,Costamagna C,Mofa F,et a1.Signaling pathway of nitric oxide-induced acrosome reaction in human spermatozoa[J].Biol Reprod,2001,64(6):1708-1712.
4. 肖春花，王忠山，左文靜，等．精子頂體酶活性檢測新方法與臨床應用[J]．男性學雜志，1994，8(4)：98-200.
5. Aquila S,Sisci D,Gentile M,et al.Towards a physiological role for cytochrome P450 aromatase in ejaculated human sperm[J].Hum Reprod,2003,18(8):1650-1659.
6. Cui YH,Zhao RL,Wang Q,et a1.Determination of sperm acrosin activity for evaluation of male fertility[J].Asian J Andm1,2000,2(3):229-232.
7. Breitbart H.Intracellular calcium regulation in sperm capacitation and acrosomal reaction[J].Mol Cell Endocrinol,2002,187(1-2):139-144.
8. Patrat C,Serresc C,Jounnet P.The aerosome reaction in human spermatozoa.Biol Cell,2000,92(3-4):255-266.
9. Luconi M,Francavilla F,Porazzi I,et a1.Human spermatozoa as a model for studying membrane receptors mediating rapid nongenomic effects of progesterone and estrogens[J].Steroids,2004,69(8-9):553-559.

West China Medical Journal

Effect and Mechanism of 17β-estradiol on Acrosome Reaction in Human Spermatozoa

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