Effect of Danggui-shaoyao-san on Expression of GATA-3 and T-bet mRNA in PBMCs_West China Medical Journal

Authors：

HUANG Zhongying , LI Shangwei , MA Qianhong

Department of Obstetrics and Gynecology,West China Second University Hospital,Sichuan University,Chengdu 610041,China;

Corresponding?author：

Keywords：

當歸芍藥散; 轉錄因子; GATA-3; T-bet; 流產

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目的：研究中藥當歸芍藥散對外周血T淋巴細胞轉錄因子GATA-3和T-bet mRNA表達的影響，初步探討其用于治療不明原因反復自然流產的可能性。方法：體外分離提取11例志愿者外周血單核細胞，在含有或不含當歸芍藥散的培養液中培養24h，用實時定量PCR技術檢測GATA-3和T-bet mRNA的表達。結果：用10 μg/mL濃度當歸芍藥散處理后,單核細胞中GATA-3 mRNA的含量與對照組比較顯著增高（P＜0.05)。當歸芍藥散處理細胞后，T-bet mRNA的表達水平呈降低趨勢，當濃度為100 μg/mL時，與對照組比較有顯著性差異（P＜0.05)。結論：當歸芍藥散可上調轉錄因子GATA-3 mRNA的表達或下調T-bet mRNA的表達,從而可能通過調節Th2/Th1平衡向Th2偏移、對于Th1反應異常增強的不明原因反復自然流產有一定的治療潛能。

Citation： HUANG Zhongying,LI Shangwei,MA Qianhong. Effect of Danggui-shaoyao-san on Expression of GATA-3 and T-bet mRNA in PBMCs. West China Medical Journal, 2008, 23(1): 27-28. doi: Copy

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1. Raghupathy R,Makhseed M,Azizieh F,et al.Maternal Th1- and Th2-type reactivity to placental antigens in normal human pregnancy and unexplained recurrent spontaneous abortions[J].Cell Immunol,1999,196(2):122-130.
2. Kwak-Kim J Y H,.Chung-Bang H S,Ng S C,et al.Increased T helper 1 cytokne responses by circulating T cells are present in women with recurrent pregnancy losses and in infertile women with multiple implantation failures after IVF.Human Reproduction,2003,18(4):767-773.
3. 劉春麗.當歸芍藥散加味治療先兆流產30例[J].安徽中醫學院學報,2000，19(4):33.
4. Zhu J,Yamane H,Cote-Sierra J,et al.GATA-3 promotes Th2 responses throughthree different mechanisms:induction of Th2 cytokine production,selective growth of Th2 cells and inhibition of Th1 cell-specific factors[J].Cell Res,2006,16(1):3-10.
5. Zheng W-P,Flavell RA.The transcription factor GATA-3 is necessary and sufficient for Th2 cytokinegene expression in CD4 T-cells[J].Cell,1997,89(4):587-596.
6. Carp H,Torchinsky A,Tein A,et al.Hormones,cytokines and fetal anomalies in habitual abortion[J].Endocrinol,2001,15(6:)472-483.
7. Usuki S,Higa TN,Soreya K.The improvement of luteal insufficiency in fecund women by tokishakuyakusan treatment[J].Am J Chin Med,2002,30(2-3):327-338.

West China Medical Journal

Effect of Danggui-shaoyao-san on Expression of GATA-3 and T-bet mRNA in PBMCs

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