The Expression of β-amyloid Precursor Protein in Brain Tissue of Pharmacoresistant Epilepsy Patients by Immunofluorescence Examination_West China Medical Journal

Authors：

JIANG Shijie ¹ , YANG Xiaofang ¹ , LI Jingmei ²

1.Department of Neurology，Sui ning People’s Hospital，Suining Sichuan，629000;2.Department of Neurology，West China Hospitall，Sichuan University，Chengdu 640041，China;

Corresponding?author：

Keywords：

難治性癲癇; 蛋白表達; 免疫熒光

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目的:β淀粉樣蛋白（β-amyloid precursor protein，β-APP）是已知的參與阿爾茨海默病機制的關鍵因子。β-APP是否參與難治性癲癇中的病理機制并不清楚。這項研究在于了解β-APP的蛋白在難治性癲癇患者術后顳葉皮質和海馬組織中的表達是否異常。方法:免疫熒光法半定量測定難治性癲癇患者術后顳葉皮質和海馬組織中的β-APP陽性蛋白的熒光值，并應用統計軟件對實驗數據進行單因素方差分析。結果:免疫熒光強度值分析結果顯示β-APP在耐藥性癲癇腦組織中表達較對照組明顯增高且有統計學意義。結論:β-APP在難治性癲癇腦組織中異常增高，增高的β-APP可能參與了難治性癲癇的病理機制。

Citation： JIANG Shijie,YANG Xiaofang,LI Jingmei. The Expression of β-amyloid Precursor Protein in Brain Tissue of Pharmacoresistant Epilepsy Patients by Immunofluorescence Examination. West China Medical Journal, 2009, 24(1): 32-34. doi: Copy

1.	Salinero O，MorenoFlores MT，Wandosell F.Increasing neurite outgrowth capacity of βamyloid precursor protein proteoglycan in Alzheimer’s disease[J].J Neurosci Res，2000，60（1）:87-97..
2.	吳遜，沈鼎烈.難治性癲癇[J].中華神經科雜志，1998，31:4..
3.	王學峰，肖波，孫紅斌.難治性癲癇[M].上海科學技術出版社，2001:5-11..
4.	Perez RG，Zheng H，Van der Ploeg LH，et al.The βamyloid precursor protein of Alzheimer’s disease enhances neuron viability and modulates neuronal polarity[J].J Neurosci，1997，17（24）:9407-9414..
5.	Niess C，Grauel U，Toennes SW，et al.Incidence of axonal injury in human brain tissue[J].Acta Neuropathol（Berl），2002，104（1）:79-84..
6.	Smith DH，Chen XH，Iwata A，et al.Amyloid β accumulation in axons after traumatic brain injury in humans[J].J Neurosurg，2003，98（5）:1072-1077..
7.	GordonKrajcer W，Gajkowska B.Excitotoxicityinduced expression of amyloid precursor protein （βAPP） in the hippocampus and cortex of rat brain.An electronmicroscopy and biochemical study[J].Folia Neuropathol，2001，39（3）:163-173..
8.	汪建華，王學峰，龔云，等.耐藥性癲癇患者術后腦組織中Tau蛋白的表達[J].中華神經科雜志，2005，38（9）:543-546..
9.	Tomizawa K，Cai XH，Moriwaki A，et al.Involvement of cyclindependent kinase 5/p35（nck5a） in the synaptic reorganization of rat hippocampus during kindling progression[J].Jpn J Physiol，2000，50（5）:525-532.
10.	Pollard H，Khrestchatisky M，Moreau J，et al.Correlation between reactive sprouting and microtubule protein expression in epileptic hippocampus[J].Nuroscience，1994，63（2）:627..
11.	Hoshi M，Sato M，Matsumoto S，et al.Spherical aggregates of βamyloid （amylospheroid） show high neurotoxicity and activate tau protein kinase Ⅰ/glycogen synthase kinase3β[J].Proc Natl Acad Sci USA，2003，100（11）:6370-6375..
12.	Han P，Dou F，Li F，et al.Suppression of cyclindependent kinase 5 activation by amyloid precursor protein:a novel excitoprotective mechanism involving modulation of tau phosphorylation[J].J Neurosci，2005，25（50）:11542-11552..
13.	Takashima A，Honda T，Yasutake K，et al.Activation of tau protein kinase Ⅰ/glycogen synthase kinase3β by amyloid β peptide （2535） enhances phosphorylation of tau in hippocampal neurons[J].Neurosci Res，1998，31（4）:317-323..
14.	Lee MS，Kwon YT，Li M，et al.Neurotoxicity induces cleavage of p35 to p25 by calpain[J].Nature，2000，405（6784）:360-364..
15.	Greenberg SM，Koo EH，Selkoe DJ，et al.Secreted βamyloid precursor protein stimulates mitogenactivated protein kinase and enhances tau phosphorylation[J].Proc Natl Acad Sci USA，1994，91（15）:7104-7108.

1. Salinero O，MorenoFlores MT，Wandosell F.Increasing neurite outgrowth capacity of βamyloid precursor protein proteoglycan in Alzheimer’s disease[J].J Neurosci Res，2000，60（1）:87-97..
2. 吳遜，沈鼎烈.難治性癲癇[J].中華神經科雜志，1998，31:4..
3. 王學峰，肖波，孫紅斌.難治性癲癇[M].上海科學技術出版社，2001:5-11..
4. Perez RG，Zheng H，Van der Ploeg LH，et al.The βamyloid precursor protein of Alzheimer’s disease enhances neuron viability and modulates neuronal polarity[J].J Neurosci，1997，17（24）:9407-9414..
5. Niess C，Grauel U，Toennes SW，et al.Incidence of axonal injury in human brain tissue[J].Acta Neuropathol（Berl），2002，104（1）:79-84..
6. Smith DH，Chen XH，Iwata A，et al.Amyloid β accumulation in axons after traumatic brain injury in humans[J].J Neurosurg，2003，98（5）:1072-1077..
7. GordonKrajcer W，Gajkowska B.Excitotoxicityinduced expression of amyloid precursor protein （βAPP） in the hippocampus and cortex of rat brain.An electronmicroscopy and biochemical study[J].Folia Neuropathol，2001，39（3）:163-173..
8. 汪建華，王學峰，龔云，等.耐藥性癲癇患者術后腦組織中Tau蛋白的表達[J].中華神經科雜志，2005，38（9）:543-546..
9. Tomizawa K，Cai XH，Moriwaki A，et al.Involvement of cyclindependent kinase 5/p35（nck5a） in the synaptic reorganization of rat hippocampus during kindling progression[J].Jpn J Physiol，2000，50（5）:525-532.
10. Pollard H，Khrestchatisky M，Moreau J，et al.Correlation between reactive sprouting and microtubule protein expression in epileptic hippocampus[J].Nuroscience，1994，63（2）:627..
11. Hoshi M，Sato M，Matsumoto S，et al.Spherical aggregates of βamyloid （amylospheroid） show high neurotoxicity and activate tau protein kinase Ⅰ/glycogen synthase kinase3β[J].Proc Natl Acad Sci USA，2003，100（11）:6370-6375..
12. Han P，Dou F，Li F，et al.Suppression of cyclindependent kinase 5 activation by amyloid precursor protein:a novel excitoprotective mechanism involving modulation of tau phosphorylation[J].J Neurosci，2005，25（50）:11542-11552..
13. Takashima A，Honda T，Yasutake K，et al.Activation of tau protein kinase Ⅰ/glycogen synthase kinase3β by amyloid β peptide （2535） enhances phosphorylation of tau in hippocampal neurons[J].Neurosci Res，1998，31（4）:317-323..
14. Lee MS，Kwon YT，Li M，et al.Neurotoxicity induces cleavage of p35 to p25 by calpain[J].Nature，2000，405（6784）:360-364..
15. Greenberg SM，Koo EH，Selkoe DJ，et al.Secreted βamyloid precursor protein stimulates mitogenactivated protein kinase and enhances tau phosphorylation[J].Proc Natl Acad Sci USA，1994，91（15）:7104-7108.

West China Medical Journal

The Expression of β-amyloid Precursor Protein in Brain Tissue of Pharmacoresistant Epilepsy Patients by Immunofluorescence Examination

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