Experimental Study on the Protective Effect of Glucosamine on Chondrocyte during Hemarthrosis_West China Medical Journal

Authors：

ZHANG Shiyang ¹ ,  ZHANG Minghua ² , JIANG Dianming ¹ , ZHAO Zenghui ¹ , LI Weichao ¹ , QIAO Bo ¹

1. Department of Orthopedics Surgery, the First Affiliated Hospital; 2. The Affiliated Yongchuan Hospital; Chongqing Medical University, Chongqing 400016, P. R. China;

Corresponding?author：

ZHANG Minghua, Email: zhang-shiyang@qq.com

Keywords：

氨基葡萄糖; 關節腔積血; 軟骨細胞; 兔

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

目的　觀察鹽酸氨基葡萄糖對兔關節腔積血造成的軟骨損傷的保護作用。方法　成年新西蘭大白兔50只，隨機分為A、B、C、D、E共5組，每組10只。A、B、C、D組采用自體靜脈血4 mL右側關節腔內注射建立關節腔積血模型。建模后，A、B、C組分別給予25、82.5、500 mg/kg的鹽酸氨基葡萄糖灌胃，1次/d；D組為陽性對照組，以6 mL/kg蒸餾水灌胃，1次/d；E組為陰性對照組，以6 mL/kg蒸餾水灌胃，1次/d。灌胃8周后取材。肉眼大體觀察，光鏡下進行Mankin評分及沙紅O染色觀察，評估軟骨損傷程度；采用免疫組織化學法測定Ⅱ型膠原蛋白（COL Ⅱ）表達情況。結果　軟骨大體觀察和沙紅O染色組織學觀察顯示，A、B、C組與D組、E組之間差異均有統計學意義（P＜0.05）。免疫組織化學提示，A、B、C組COL Ⅱ表達明顯高于D組（P＜0.05）。結論　鹽酸氨基葡萄糖對兔關節腔積血導致的軟骨損傷有一定的保護治療作用，在防治關節腔積血導致的軟骨損傷的發生發展方面，具有一定的作用。

Citation： ZHANG Shiyang,ZHANG Minghua,JIANG Dianming,ZHAO Zenghui,LI Weichao,QIAO Bo. Experimental Study on the Protective Effect of Glucosamine on Chondrocyte during Hemarthrosis. West China Medical Journal, 2012, 27(12): 1788-1791. doi: Copy

1.	Roosendaal G, Vianen ME, Marx JJ, et al. Blood-induced joint damage: a human in vitro study[J]. Arthritis Rheum, 1999, 42(5): 1025-1032.
2.	Lohmander LS, injury RL. Surgery and osteoarthrosis: truth or Consequences[J]. Acta Orthop Scand, 1994, 65(2): 605-609.
3.	Da CC, Dowless GV. Glucosamine sulfate for osteoarthritis[J]. Ann Pharmaeother, 1998, 32(5): 580-587.
4.	Roosendaal G, Tekoppele JM, Vianen ME, et al. Blood-induced joint damage: a canine in vitro study[J]. Arthritis Rheum, 1999, 42(5): 1033-1039.
5.	Lipowitz AJ, Wong PL, Stevens JB. Synovial membrane changes after experimental transection of the cranial cruciate ligament in dogs[J]. Am J Vet Res, 1985, 46(5): 1166-1170.
6.	Mankin HJ, Dorfman H, Lippiello L, et al. Biochemical and metabolic abnormalities in articular cartilage from osteo-arthritic human hips. II. Correlation of morphology with biochemical and metabolic data[J]. J Bone Joint Surg Am, 1971, 53(3): 523-537.
7.	Michel JJ, Goris R, Marieke E, et al. Immature articular cartilage is more susceptible to blood-induced damage than mature articular cartilage:An in vivo animal study[J]. Arthritis Rheum, 2010, 48(2): 396-403.
8.	Valentino LA. Blood-induced joint disease: the pathophysiology of hemophilic arthropathy[J]. J Thromb Haemost, 2010, 8(9): 1895-1902.
9.	張繼春, 陳金棟, 陳百成, 等. 兔關節內積血對關節軟骨影響的實驗研究[J]. 中國康復醫學雜志, 2007, 22(1): 31-33.
10.	Hakobyan N. Kazarian T’valentino LA.Synovitis in a murine model of human factor Ⅷdeficiency[J]. Haemophilia, 2005, 11(3): 227-232.
11.	Tan AH, Mitra AK, Chang PC, et al. Assessment of blood-induced cartilage damage in rabbit knees using scanning electron microscopy[J]. J Orthop Surg (Hong Kong), 2004, 12(2): 199-204.
12.	Hakobyan N, Kazarian T, Valentino LA. Synovitis in a murine model of human factor Ⅷ deficiency[J]. Haemophilia, 2005, 11(3): 227-232.
13.	傅欣, 林霖, 魏學磊, 等. 氨基葡萄糖和硫酸軟骨素聯合用藥對兔骨關節炎治療作用實驗研究[J]. 中國運動醫學雜志, 2008, 22(5): 588-592.
14.	Piperno M, Reboul P, Hellio Le Graverand MP, et al. Glucosamine sulfate modulates dysregulated activities of human osteoarthritic chondrocytes in vitro[J]. Osteoarthritis Cartilage, 2000, 8(3): 207-212.
15.	Nakamura H, Shibakawa A, Tanaka M, et al. Effects of glucosamine hydrochloride on the production of prostaglandin E2, nitric oxide and metalloproteases by chondrocytes and synoviocytes in osteoarthritis[J]. Clin Exp Rheumatol, 2004, 22(3): 293-299.
16.	楊南萍, 李江濤, 陳永濤, 等. 多中心隨機雙盲雙模擬陽性藥平行對照臨床研究:評價國產鹽酸氨基葡萄糖片在治療骨關節炎中的有效性和安全性[J]. 華西醫學, 2005, 20(4): 54-56.
17.	Bassleer C, Rovati L, Franchimont P. Stimulation of proteoglycan production by glucosamine sulfate in chondrocytes isolated from human osteoarthritic articular cartilage in vitro[J]. Osteoarthritis Cartilage, 1998, 6(6): 427-434.
18.	Qiu GX, Gao SN, Giacovelli G, et al. Efficacy and safety of glucosamine sulfate versus ibuprofen in patients with knee osteoarthritis[J]. Arzneimittelforschung, 1998, 48(5): 469-474.
19.	王曉濱, 黃公怡, 薛慶云. 硫酸氨基葡萄糖抑制白介素1β誘導的人骨關節炎軟骨細胞合成一氧化氮研究[J]. 中華老年醫學雜志, 2012, 31(2): 140-143.
20.	Largo R, Alvarez-Soria MA, Díez-Ortego I, et al. Glucosamine inhibits IL-1beta-induced NFkappaB activation in human osteoarthritic chondrocytes[J]. Osteoarthritis Cartilage, 2003, 11(4): 290-298.
21.	Huser CA, Davies ME. Effect of a glucosamine derivative on impact-induced chondrocyte apoptosis in vitro. A preliminary report[J]. Osteoarthritis Cartilage, 2008, 16(1): 125-128.

1. 　Roosendaal G, Vianen ME, Marx JJ, et al. Blood-induced joint damage: a human in vitro study[J]. Arthritis Rheum, 1999, 42(5): 1025-1032.
2. 　Lohmander LS, injury RL. Surgery and osteoarthrosis: truth or Consequences[J]. Acta Orthop Scand, 1994, 65(2): 605-609.
3. 　Da CC, Dowless GV. Glucosamine sulfate for osteoarthritis[J]. Ann Pharmaeother, 1998, 32(5): 580-587.
4. 　Roosendaal G, Tekoppele JM, Vianen ME, et al. Blood-induced joint damage: a canine in vitro study[J]. Arthritis Rheum, 1999, 42(5): 1033-1039.
5. 　Lipowitz AJ, Wong PL, Stevens JB. Synovial membrane changes after experimental transection of the cranial cruciate ligament in dogs[J]. Am J Vet Res, 1985, 46(5): 1166-1170.
6. 　Mankin HJ, Dorfman H, Lippiello L, et al. Biochemical and metabolic abnormalities in articular cartilage from osteo-arthritic human hips. II. Correlation of morphology with biochemical and metabolic data[J]. J Bone Joint Surg Am, 1971, 53(3): 523-537.
7. 　Michel JJ, Goris R, Marieke E, et al. Immature articular cartilage is more susceptible to blood-induced damage than mature articular cartilage:An in vivo animal study[J]. Arthritis Rheum, 2010, 48(2): 396-403.
8. 　Valentino LA. Blood-induced joint disease: the pathophysiology of hemophilic arthropathy[J]. J Thromb Haemost, 2010, 8(9): 1895-1902.
9. 　張繼春, 陳金棟, 陳百成, 等. 兔關節內積血對關節軟骨影響的實驗研究[J]. 中國康復醫學雜志, 2007, 22(1): 31-33.
10. 　Hakobyan N. Kazarian T’valentino LA.Synovitis in a murine model of human factor Ⅷdeficiency[J]. Haemophilia, 2005, 11(3): 227-232.
11. 　Tan AH, Mitra AK, Chang PC, et al. Assessment of blood-induced cartilage damage in rabbit knees using scanning electron microscopy[J]. J Orthop Surg (Hong Kong), 2004, 12(2): 199-204.
12. 　Hakobyan N, Kazarian T, Valentino LA. Synovitis in a murine model of human factor Ⅷ deficiency[J]. Haemophilia, 2005, 11(3): 227-232.
13. 　傅欣, 林霖, 魏學磊, 等. 氨基葡萄糖和硫酸軟骨素聯合用藥對兔骨關節炎治療作用實驗研究[J]. 中國運動醫學雜志, 2008, 22(5): 588-592.
14. 　Piperno M, Reboul P, Hellio Le Graverand MP, et al. Glucosamine sulfate modulates dysregulated activities of human osteoarthritic chondrocytes in vitro[J]. Osteoarthritis Cartilage, 2000, 8(3): 207-212.
15. 　Nakamura H, Shibakawa A, Tanaka M, et al. Effects of glucosamine hydrochloride on the production of prostaglandin E2, nitric oxide and metalloproteases by chondrocytes and synoviocytes in osteoarthritis[J]. Clin Exp Rheumatol, 2004, 22(3): 293-299.
16. 　楊南萍, 李江濤, 陳永濤, 等. 多中心隨機雙盲雙模擬陽性藥平行對照臨床研究:評價國產鹽酸氨基葡萄糖片在治療骨關節炎中的有效性和安全性[J]. 華西醫學, 2005, 20(4): 54-56.
17. 　Bassleer C, Rovati L, Franchimont P. Stimulation of proteoglycan production by glucosamine sulfate in chondrocytes isolated from human osteoarthritic articular cartilage in vitro[J]. Osteoarthritis Cartilage, 1998, 6(6): 427-434.
18. 　Qiu GX, Gao SN, Giacovelli G, et al. Efficacy and safety of glucosamine sulfate versus ibuprofen in patients with knee osteoarthritis[J]. Arzneimittelforschung, 1998, 48(5): 469-474.
19. 　王曉濱, 黃公怡, 薛慶云. 硫酸氨基葡萄糖抑制白介素1β誘導的人骨關節炎軟骨細胞合成一氧化氮研究[J]. 中華老年醫學雜志, 2012, 31(2): 140-143.
20. 　Largo R, Alvarez-Soria MA, Díez-Ortego I, et al. Glucosamine inhibits IL-1beta-induced NFkappaB activation in human osteoarthritic chondrocytes[J]. Osteoarthritis Cartilage, 2003, 11(4): 290-298.
21. 　Huser CA, Davies ME. Effect of a glucosamine derivative on impact-induced chondrocyte apoptosis in vitro. A preliminary report[J]. Osteoarthritis Cartilage, 2008, 16(1): 125-128.

West China Medical Journal

Experimental Study on the Protective Effect of Glucosamine on Chondrocyte during Hemarthrosis

Abstract Full text Figures/Tables Video References Cited by

Format

Content