Study of recombinant human erythropoietin on hippocampal proteomics in pentylenetetrazole-induced epileptic rats_Journal of Epilepsy

Authors：

ZHANG Wenjuan ¹ , WANG Guangwen ² ,  YANG Jianzhong ¹

1. Department of neurology, Shanxi Provincial Peoples, Hospital, Taiyuan 030012, China;
2. Department of Neurology, General Hospital of Taiyuan Iron & Steel CO., LTD. (TISCO), Taiyuan 030003, China;

Corresponding?author：

YANG Jianzhong, Email: 1595021181@qq.com

Keywords：

Recombinant human erythropoietin; SD rats; Hippocampus; Proteomics

DOI：

10.7507/2096-0247.202207006

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To study the differentially expressed proteins of recombinant human erythropoietin (r-HuEPO) in hippocampus of Pentetrazol (PTZ) -induced epileptic rats, and to provide a basis for exploring the pathogenesis of epilepsy and seeking new therapeutic targets. Methods Twelve 6~8-week-old Sprague Dawley rats that weighted 230~250 g were randomly divided into two groups: PTZ group, PTZ+ EPO group. The differential proteins of recombinant human EPO in hippocampus of pentylenetetrazole-induced epileptic rats were analyzed and identified by TMT technique based on mass spectrometry.Results 139 differentially expressed protein sites were detected in hippocampal tissues of epileptic rats, of which 55 were up-regulated and 84 down-regulated. Conclusion Recombinant human erythropoietin can inhibit many differentially expressed proteins in the hippocampus of pentaerythraze-induced eclampsia rats by upregulation of Isocitrate dehydrogenase (NADP), Reduced nicotinamide purine dinucleotide phosphate (NADPH), Thioredoxin reductase 2 mitochondrial (TrxR), reduce nerve cell damage.

Citation： ZHANG Wenjuan, WANG Guangwen, YANG Jianzhong. Study of recombinant human erythropoietin on hippocampal proteomics in pentylenetetrazole-induced epileptic rats. Journal of Epilepsy, 2022, 8(6): 529-534. doi: 10.7507/2096-0247.202207006 Copy

1.	Roland D Thijs, Rainer Surges, Terence J O'Brien, et al. Epilepsy in adults. Lancet, 2019, 393(10172): 689-701.
2.	張淑琴, 林衛紅, 趙麗曼. 實驗性癲癇不同腦區細胞凋亡的觀察及意義. 中風與神經疾病雜志, 2002, 19(4): 205-207.
3.	Beng zon J, Mohapel P, Ekdahl CT, et al. Neuronal apoptosis after brief and prolonged seizures. Prog Brain Res, 2002, 135: 111-119.
4.	Stadtman ER. Protein oxidation in aging and age-related diseases. Ann N. Y. Acad. Sci, 2001, 928: 22-38.
5.	Wong-ekkabut J, Xu Z, Triampo W, et al. Effect of lipid peroxidation on the properties of lipid bilayers: a molecular dynamics study. Biophys J, 2007, 93: 4225-4236.
6.	Henshall DC, Murphy BM. Modulators of neuronal cell death in epilepsy. Curr Opin Pharmacol, 2008, 8(1): 75-81.
7.	宋修云, 胡金鳳, 陳乃宏. 神經細胞凋亡與腦缺血疾病. 中國藥理學通報, 2012, 28(3): 307-310. .
8.	王維平, 史志勤, 于江華等. 重組人紅細胞生成素對癲癇持續狀態大鼠海馬p-Akt和caspase-9的影響及作用機制. 南方醫科大學學報, 2010, 30(1): 64-69.
9.	史志勤, 王維平, 于江華, 等. 重組人紅細胞生成素經PI3K/Akt途徑對大鼠癲癇持續狀態誘導的神經元凋亡的保護作用. 中國組織化學與細胞化學雜志, 2009, 18(1): 94-102.
10.	張思遠, 蔡昕, 盧曉樺, 等. 新型H2S供體激活PI3K/Akt對癲癇大鼠腦內Bcl-2/Bax蛋白表達的影響. 華中科技大學學報(醫學版), 2020, 49(3): 296-300.
11.	Singh A, Kukreti R, Saso L. Oxidative stress: a key modulator in neurodegenerative disease. Molecules, 2019, 24(8): 1583.
12.	Waldbaum S, Patel M. Mitochondrial oxidative stress in temporal lobe epilepsy. Epilepsy Res, 2010, 88(1): 23-45.
13.	榮佳, 崔亞洲, 周小艷, 等. 顳葉癲癇大鼠模型海馬蛋白質組學的研究. 山東大學學報(醫學版), 2011, 49(2): 39-43,53.
14.	孫飛, 周強軍, 孫吉, 等. 線粒體呼吸鏈膜蛋白復合體的結構. 生命科學, 2008, 20(4): 566-578.
15.	陳海浪, 陳喜文, 陳德富. NAD+-依賴型異檸檬酸脫氫酶的結構和功能研究進展. 生物技術通訊, 2003, 14(4): 304-307,311.
16.	Lu J, Holmgren A. The thioredoxin antioxidant system. Free Radic Biol Med, 2014, 66: 75-87.
17.	周潤梅. 硫氧還蛋白還原酶的生理學功能研究進展. 現代醫藥衛生, 2012, 28(16): 2487-2488.
18.	Lee S, Kim SM, LEE RT. Thioredoxin and thioredoxin target proteins: from molecular mechanisms to functional significance. Antioxid Redox Signal, 2013, 18(10): 1165-1207.
19.	Nilanjana Maulik, Dipak K Das. Emerging potential of thioredoxin and thioredoxin interacting proteins in various disease conditions. Biochim Biophys Acta, 2008, 1780(11): 1368-1382.
20.	L. Tao E, Gao NS, Bryan T, et al Cardioprotective effects ofthioredoxin in myocardial ischemia and reperfusion: roleof S-nitrosation [corrected]. Proc Natl Acad Sci USA, 2004, 101: 11471-11476.
21.	魏學文, 張曉偉. 癲癇大鼠海馬硫氧還蛋白還原酶活性及cleaved caspase-3表達檢測. 檢驗醫學與臨床, 2011, 8(22): 2695-2696.

1. Roland D Thijs, Rainer Surges, Terence J O'Brien, et al. Epilepsy in adults. Lancet, 2019, 393(10172): 689-701.
2. 張淑琴, 林衛紅, 趙麗曼. 實驗性癲癇不同腦區細胞凋亡的觀察及意義. 中風與神經疾病雜志, 2002, 19(4): 205-207.
3. Beng zon J, Mohapel P, Ekdahl CT, et al. Neuronal apoptosis after brief and prolonged seizures. Prog Brain Res, 2002, 135: 111-119.
4. Stadtman ER. Protein oxidation in aging and age-related diseases. Ann N. Y. Acad. Sci, 2001, 928: 22-38.
5. Wong-ekkabut J, Xu Z, Triampo W, et al. Effect of lipid peroxidation on the properties of lipid bilayers: a molecular dynamics study. Biophys J, 2007, 93: 4225-4236.
6. Henshall DC, Murphy BM. Modulators of neuronal cell death in epilepsy. Curr Opin Pharmacol, 2008, 8(1): 75-81.
7. 宋修云, 胡金鳳, 陳乃宏. 神經細胞凋亡與腦缺血疾病. 中國藥理學通報, 2012, 28(3): 307-310. .
8. 王維平, 史志勤, 于江華等. 重組人紅細胞生成素對癲癇持續狀態大鼠海馬p-Akt和caspase-9的影響及作用機制. 南方醫科大學學報, 2010, 30(1): 64-69.
9. 史志勤, 王維平, 于江華, 等. 重組人紅細胞生成素經PI3K/Akt途徑對大鼠癲癇持續狀態誘導的神經元凋亡的保護作用. 中國組織化學與細胞化學雜志, 2009, 18(1): 94-102.
10. 張思遠, 蔡昕, 盧曉樺, 等. 新型H2S供體激活PI3K/Akt對癲癇大鼠腦內Bcl-2/Bax蛋白表達的影響. 華中科技大學學報(醫學版), 2020, 49(3): 296-300.
11. Singh A, Kukreti R, Saso L. Oxidative stress: a key modulator in neurodegenerative disease. Molecules, 2019, 24(8): 1583.
12. Waldbaum S, Patel M. Mitochondrial oxidative stress in temporal lobe epilepsy. Epilepsy Res, 2010, 88(1): 23-45.
13. 榮佳, 崔亞洲, 周小艷, 等. 顳葉癲癇大鼠模型海馬蛋白質組學的研究. 山東大學學報(醫學版), 2011, 49(2): 39-43,53.
14. 孫飛, 周強軍, 孫吉, 等. 線粒體呼吸鏈膜蛋白復合體的結構. 生命科學, 2008, 20(4): 566-578.
15. 陳海浪, 陳喜文, 陳德富. NAD+-依賴型異檸檬酸脫氫酶的結構和功能研究進展. 生物技術通訊, 2003, 14(4): 304-307,311.
16. Lu J, Holmgren A. The thioredoxin antioxidant system. Free Radic Biol Med, 2014, 66: 75-87.
17. 周潤梅. 硫氧還蛋白還原酶的生理學功能研究進展. 現代醫藥衛生, 2012, 28(16): 2487-2488.
18. Lee S, Kim SM, LEE RT. Thioredoxin and thioredoxin target proteins: from molecular mechanisms to functional significance. Antioxid Redox Signal, 2013, 18(10): 1165-1207.
19. Nilanjana Maulik, Dipak K Das. Emerging potential of thioredoxin and thioredoxin interacting proteins in various disease conditions. Biochim Biophys Acta, 2008, 1780(11): 1368-1382.
20. L. Tao E, Gao NS, Bryan T, et al Cardioprotective effects ofthioredoxin in myocardial ischemia and reperfusion: roleof S-nitrosation [corrected]. Proc Natl Acad Sci USA, 2004, 101: 11471-11476.
21. 魏學文, 張曉偉. 癲癇大鼠海馬硫氧還蛋白還原酶活性及cleaved caspase-3表達檢測. 檢驗醫學與臨床, 2011, 8(22): 2695-2696.

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Study of recombinant human erythropoietin on hippocampal proteomics in pentylenetetrazole-induced epileptic rats

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