The study of electroencephalogram and magnetic resonance imaging about Wistar suckling rats Focal cortical dysplasia model_Journal of Epilepsy

Authors：

 GUOTao , CHENGYao , WUJiang

Department of Functional Neurosurgery, He Bei General Hospital, Shi jia zhuang 050051, China;

Corresponding?author：

GUOTao, Email: 8888guotao@sina.com

Keywords：

Wistar suckling rats; Focal cortical dysplasia; Animal model; Electroencephalogram; Magnetic resonance imaging

DOI：

10.7507/2096-0247.20160089

Video：

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Objective To make the model of Wistar suckling rats Focal cortical dysplasia (FCD) by liquid nitrogen freezing brain cortex and verify it. Analysed the electroencephalogram (EEG) and magnetic resonance imaging (MRI) features of the FCD model, in order to provide theoretical and experimental basis for human FCD diagnosis and treatment. Methods Take the first day of Wistar suckling rats as experimental object, liquid nitrogen freezing Wistar suckling rats brain cortex.Make examination of EEG and MRI for Wistar suckling rats. The Brain tissue slice of Wistar suckling rats model dyed by HE and check with light microscope examination. Results In experiment group, the sample epileptic discharge rate of EEG was about 41.6% on average, and showed visible spike wave, spine slow wave frequency distribution. Experimental Wistar suckling rats MRI showed positive performance for long T1 and long T2 signal, brain tissue slices HE staining showed brain cortex layer structure and columnar structure disorder, exist abnormal neurons and the balloon sample cells. Conclusion The method of liquid nitrogen freezing Wistar suckling rats cortex can established FCDⅢd animal models successfully, and showed specific EEG and MRI, which has important value for diagnosis and treatment of human FCD.

Citation： GUOTao, CHENGYao, WUJiang. The study of electroencephalogram and magnetic resonance imaging about Wistar suckling rats Focal cortical dysplasia model. Journal of Epilepsy, 2016, 2(6): 504-508. doi: 10.7507/2096-0247.20160089 Copy

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5.	孫基棟, 王丹丹, 梁暉, 等. 難治性癲癇172 例臨床病理分析. 中華神經外科雜志, 2010, 26(6):494-496.
6.	Knchnkhidze G,Koppelstaetter F, Unterberaer 1, et al. Midbrain-hindbrain malformations in patients with malformations of cortical development and epilepsy:a series of 220 patients. Epilepsy Research, 2013,106(1-2):181-190.
7.	Barkovich AJ,Guerrini R,Kuzniecky RI,et al.A developmental and genetic classification for malformations of cortical development:update 2012. Brain, 2012, 135(Pt 5):1348-1369.
8.	郭韜, 孫吉林, 昊育錦, 等. 影像學難以確診的額葉癲癇的診斷與定位. 中華神經外科雜志, 2013, 29(12):1224-1272.
9.	Guerrini R, Dobyns WB. Malformations of cortical development:clinical features and genetic causes.The Lancet Neurology, 2014,13(7):710-726.
10.	Barkovich A.I,Guerrini H,Kuzniecky RI, et al. A developmentaland genetic classification for malformations of cortical development:update 2012. Brain, 2012, 135(5):1348-1369.
11.	Bast T, Ramantani G, Seitz A, et al. Focal cortical dysplasia:prevalence, clinical presentation and epilepsy in children and adults. Acta Neurol Scand, 2006, 113(4):72-81.
12.	盛卸晃, 宋淑亮, 梁錦鋒, 等. 膠質瘢痕對神經系統損傷的保護作用. 生命的化學, 2011, 31 (2):272-275.
13.	孫吉林, 趙文清,田中雨,等.腦軟化灶FLAIR高信號與癲癇灶關系初探.中國臨床醫學影像雜志, 2009, 20(10):733-735.

1. Blumcke I,Muhlebner A. Neuropathological work-up of focalcortical dysplasias using the new ILAE consensusclassification system-practical guideline article invited by theEuro-CNS research committee. Clin Neuropathol, 2011, 30(4):164-177.
2. Blumcke I,Thom M,Aronica E,et al. The clinicopathologic spectrum of focal cortical dysplasias:a consensus classification proposed by an ad hoc Task Force of the ILAE Diagnostic Methods Commission. Epilepsia, 2011, 52(1):158-174.
3. Kuba R,Tyrlikova I,Chrastina J,et al. MRI-negative PET-positive temporal lobe epilepsy:invasive EEG findings, histopathology and postoperative outcomes. Epilepsy Behav, 2011, 22(7):537-541.
4. 郭韜, 康進生, 鄭杰, 等. 嗎啡急性成癮貓腦電圖及神經病理特點. 中華神經醫學雜志, 2011, 11(2):137-141.
5. 孫基棟, 王丹丹, 梁暉, 等. 難治性癲癇172 例臨床病理分析. 中華神經外科雜志, 2010, 26(6):494-496.
6. Knchnkhidze G,Koppelstaetter F, Unterberaer 1, et al. Midbrain-hindbrain malformations in patients with malformations of cortical development and epilepsy:a series of 220 patients. Epilepsy Research, 2013,106(1-2):181-190.
7. Barkovich AJ,Guerrini R,Kuzniecky RI,et al.A developmental and genetic classification for malformations of cortical development:update 2012. Brain, 2012, 135(Pt 5):1348-1369.
8. 郭韜, 孫吉林, 昊育錦, 等. 影像學難以確診的額葉癲癇的診斷與定位. 中華神經外科雜志, 2013, 29(12):1224-1272.
9. Guerrini R, Dobyns WB. Malformations of cortical development:clinical features and genetic causes.The Lancet Neurology, 2014,13(7):710-726.
10. Barkovich A.I,Guerrini H,Kuzniecky RI, et al. A developmentaland genetic classification for malformations of cortical development:update 2012. Brain, 2012, 135(5):1348-1369.
11. Bast T, Ramantani G, Seitz A, et al. Focal cortical dysplasia:prevalence, clinical presentation and epilepsy in children and adults. Acta Neurol Scand, 2006, 113(4):72-81.
12. 盛卸晃, 宋淑亮, 梁錦鋒, 等. 膠質瘢痕對神經系統損傷的保護作用. 生命的化學, 2011, 31 (2):272-275.
13. 孫吉林, 趙文清,田中雨,等.腦軟化灶FLAIR高信號與癲癇灶關系初探.中國臨床醫學影像雜志, 2009, 20(10):733-735.

Journal of Epilepsy

The study of electroencephalogram and magnetic resonance imaging about Wistar suckling rats Focal cortical dysplasia model

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