The investigation of genotypes of HCV and clinical characteristics in HCV patients in West China Hospital of Sichuan University_Chinese Journal of Evidence-Based Medicine

Authors：

WANG Xuemei , ZHOU Juan , YE Yuanxin , YANG Lian , ZHOU Yi , LU Xiaojun ,  YING Binwu

Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, 610041, P.R.China;

Corresponding?author：

YING Binwu, Email: binwuying@126.com

Keywords：

West China Hospital of Sichuan University; Hepatitis C virus; Genotype

DOI：

10.7507/1672-2531.201611041

Video：

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Objective To investigate HCV genotypes in HCV patients in West China Hospital of Sichuan University, and to analyze the major genotypes and clinical characteristics. Methods From March 2011 to September 2016, 4 520 HCV patients who were successfully genotyped HCV genotypes were enrolled in West China Hospital of Sichuan University. The genotypes distributions and the characteristics of laboratory characteristics of liver function, the viral loading were all analyzed. In addition, the genotypes in HCC patients, liver cirrhosis, HBC/HCV co-infection were also analyzed. Results HCV genotypes of HCV patients were divided into five genotypes of 1, 2, 3, 4, 6 and 23 subtypes, including predominant genotypes/subtypes 1b, 1*, 3b, 2a, 3a and 6a, accounting for 66.42%, 8.01%, 6.57%, 4.54%, 4.29%, and 3.41%, respectively. Subtype 1b was the predominant subtype for both sex. In male patients, the levels of ALT were highest in 6a subtype, while in female, the levels of ALT were highest in 3a subtype. For the 94 liver cirrhosis patients, 42 patients were 1b subtypes; as for the 6 HCC patients, 1b and 3b subtypes were the only detected. Conclusion HCV genotypes/subtypes of HCV patients in West China Hospital of Sichuan University have unique characteristics of distribution, while the predominant genotype/subtypes are 1b,1*, 3b, 2a, 3a, 6a.

Citation： WANG Xuemei, ZHOU Juan, YE Yuanxin, YANG Lian, ZHOU Yi, LU Xiaojun, YING Binwu. The investigation of genotypes of HCV and clinical characteristics in HCV patients in West China Hospital of Sichuan University. Chinese Journal of Evidence-Based Medicine, 2017, 17(5): 511-516. doi: 10.7507/1672-2531.201611041 Copy

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6.	中華醫學會肝病學分會. 丙型肝炎防治指南 (2015 更新版). 中華傳染病雜志, 2015, 20(12): 192-194.
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8.	Wang CB, Cheng ZX, Chen JJ, et al. Epidemiological characteristics and risk factors of hepatitis C virus genotype 1 infection: a national epidemiological survey of Chinese Han population. Eur Rev Med Pharmacol Sci, 2016, 20(6): 1052-1056.
9.	王敏, 陳錦艷, 許茹, 等. 廣州地區獻血人群中 HCV 感染者 HCV 基因分型及感染途徑研究. 中國輸血雜志, 2016, 29(1): 25-28.
10.	Taherkhani R, Farshadpour F. Epidemiology of hepatitis C virus in Iran. World J Gastroenterol, 2015, 21(38): 10790-10810.
11.	Triantos C, Konstantakis C, Tselekouni P, et al. Epidemiology of hepatitis C in Greece. World J Gastroenterol, 2016, 22(36): 8094-8102.
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13.	Sadeghi F, Salehi-Vaziri M, Almasi-Hashiani A, et al. Prevalence of Hepatitis C Virus Genotypes Among Patients in Countries of the Eastern Mediterranean Regional Office of WHO (EMRO) : A Systematic Review and Meta-Analysis. Hepat Mon, 2016, 16(4): e35558.
14.	Petruzziello A, Marigliano S, Loquercio G, et al. Hepatitis C virus (HCV) genotypes distribution: an epidemiological up-date in Europe. Infect Agent Cancer, 2016, 11: 53.
15.	Lim SG, Dan YY. A 2015 roadmap for the management of hepatitis C virus infections in Asia. Korean J Intern Med, 2015, 30(4): 423-433.
16.	Chen W, Liao B, Hu F, et al. Changing Epidemiology of Hepatitis C Virus Genotype among Patients with Human Immunodeficiency Virus/Hepatitis C Virus Co-Infection in China. PLoS One, 2016, 11(9): e161844.
17.	陳旭, 蘇明華, 江建寧, 等. 廣西地區 HIV 合并 HCV 感染人群 HCV 基因型分布特征. 中國艾滋病性病, 2016, 22(8): 593-596.
18.	張宇, 柳麗娟, 張啟云. 福建地區慢性丙型肝炎患者丙型肝炎病毒基因分型研究. 福建醫藥雜志, 2016, 38(2): 91-94.
19.	Wu Z, Cui L, Zhao W, et al. Molecular epidemiology of hepatitis C infections in Ningxia, China: genotype, phylogeny and mutation analysis. Virol J, 2016, 13(1): 172.
20.	肖桂榮, 徐珽. 2009～2013 年四川大學華西醫院 1 998 株丙型肝炎病毒基因分型研究. 四川大學學報(醫學版), 2016, 47(5): 755-758.
21.	Vilibic-Cavlek T, Kucinar J, Kaic B, et al. Epidemiology of hepatitis C in Croatia in the European context. World J Gastroenterol, 2015, 21(32): 9476-9493.
22.	Wan Z, Chen Q, Chen X, et al. HCV Diversity among Chinese and Burmese IDUs in Dehong, Yunnan, China. PLoS One, 2016, 11(9): e163062.
23.	Yang L, Jiang C, Hu S, et al. Evolving Diversity of Hepatitis C Viruses in Yunnan Honghe, China. Int J Mol Sci, 2016, 17(3): 403.
24.	Wu T, Xiong L, Wang F, et al. A Unique Pattern of HCV Genotype Distribution on Hainan Island in China Revealed by Evolutionary Analysis. Cell Physiol Biochem, 2016, 39(1): 316-330.
25.	Niu Z, Zhang P, Tong Y. Age and gender distribution of Hepatitis C virus prevalence and genotypes of individuals of physical examination in WuHan, Central China. Springerplus, 2016, 5(1): 1557.
26.	丁柳, 鐘慧鈺, 宋興勃, 等. 四川人群丙肝病毒基因型分布及與抗病毒療效的相關性. 四川大學學報（醫學版）, 2015, 46(4): 573-577.
27.	林江, 溫先勇, 唐敏. 川南地區漢族人群丙型肝炎病毒基因分型及其與臨床關系. 檢驗醫學與臨床, 2016, 13(10): 1359-1361.
28.	Freeman AJ, Dore GJ, Law MG, et al. Estimating progression to cirrhosis in chronic hepatitis C virus infection. Hepatology, 2001, 34(4 Pt 1): 809-816.
29.	Li J F, Liu S, Ren F, et al. Fibrosis progression in interferon treatment-naive Chinese plasma donors with chronic hepatitis C for 20 years: a cohort study. Int J Infect Dis, 2014, 27: 49-53.
30.	Rao H Y, Sun D G, Yang R F, et al. Outcome of hepatitis C virus infection in Chinese paid plasma donors: a 12-19-year cohort study. J Gastroenterol Hepatol, 2012, 27(3): 526-532.
31.	Kenny-Walsh E. Clinical outcomes after hepatitis C infection from contaminated anti-D immune globulin. Irish Hepatology Research Group. N Engl J Med, 1999, 340(16): 1228-1233.
32.	El-Serag HB, Rudolph KL. Hepatocellular carcinoma: epidemiology and molecular carcinogenesis. Gastroenterology, 2007, 132(7): 2557-2576.
33.	Yan LB, Rao HY, Ma YJ, et al. Hepatitis B virus infection in Chinese patients with hepatitis C virus infection: prevalence, clinical characteristics, viral interactions and host genotypes: a nationwide cross-sectional study. BMJ Open, 2016, 6(10): e12016.
34.	Simmonds P, Bukh J, Combet C, et al. Consensus proposals for a unified system of nomenclature of hepatitis C virus genotypes. Hepatology, 2005, 42(4): 962-973.
35.	Bouchardeau F, Cantaloube J F, Chevaliez S, et al. Improvement of hepatitis C virus (HCV) genotype determination with the new version of the INNO-LiPA HCV assay. J Clin Microbiol, 2007, 45(4): 1140-1145.
36.	Yang R, Cong X, Du S, et al. Performance comparison of the versant HCV genotype 2.0 assay (LiPA) and the abbott realtime HCV genotype II assay for detecting hepatitis C virus genotype 6. J Clin Microbiol, 2014, 52(10): 3685-3692.
37.	楊靜靜. 丙型肝炎病毒基因型及 F 蛋白與丙型肝炎慢性化、肝癌細胞凋亡的關系研究. 南京: 南京醫科大學, 2009.
38.	Cui Y, Jia J. Update on epidemiology of hepatitis B and C in China. J Gastroenterol Hepatol, 2013, 28 (Suppl 1): 7-10.
39.	Silini E, Bono F, Cerino A, et al. Virological features of hepatitis C virus infection in hemodialysis patients. J Clin Microbiol, 1993, 31(11): 2913-2917.
40.	Janjua NZ, Chong M, Kuo M, et al. Long-term effect of sustained virological response on hepatocellular carcinoma in patients with hepatitis C in Canada. J Hepatol, 2017, 66(3)504-513.
41.	Zaltron S, Spinetti A, Biasi L, et al. Chronic HCV infection: epidemiological and clinical relevance. BMC Infect Dis, 2012,12(Suppl 2): S2.
42.	Chemaitelly H, Chaabna K, Abu-Raddad LJ. The Epidemiology of Hepatitis C Virus in the Fertile Crescent: Systematic Review and Meta-Analysis. PLoS One, 2015, 10(8): e135281.

1. ?Smith DB, Bukh J, Kuiken C, et al. Expanded classification of hepatitis C virus into 7 genotypes and 67 subtypes: updated criteria and genotype assignment web resource. Hepatology, 2014, 59(1): 318-327.
2. Gower E, Estes C, Blach S, et al. Global epidemiology and genotype distribution of the hepatitis C virus infection. J Hepatol, 2014, 61(Suppl 1): S45-S57.
3. Sievert W, Altraif I, Razavi HA, et al. A systematic review of hepatitis C virus epidemiology in Asia, Australia and Egypt. Liver Int, 2011,31(Suppl 2): 61-80.
4. Mohd HK, Groeger J, Flaxman AD, et al. Global epidemiology of hepatitis C virus infection: new estimates of age-specific antibody to HCV seroprevalence. Hepatology, 2013, 57(4): 1333-1342.
5. Lee MH, Yang HI, Yuan Y, et al. Epidemiology and natural history of hepatitis C virus infection. World J Gastroenterol, 2014, 20(28): 9270-9280.
6. 中華醫學會肝病學分會. 丙型肝炎防治指南 (2015 更新版). 中華傳染病雜志, 2015, 20(12): 192-194.
7. 陳紅松, 竇曉光, 段鐘平, 等. 丙型肝炎防治指南 (2015 年更新版). 中國病毒病雜志, 2015, 29(6): 1961-1979.
8. Wang CB, Cheng ZX, Chen JJ, et al. Epidemiological characteristics and risk factors of hepatitis C virus genotype 1 infection: a national epidemiological survey of Chinese Han population. Eur Rev Med Pharmacol Sci, 2016, 20(6): 1052-1056.
9. 王敏, 陳錦艷, 許茹, 等. 廣州地區獻血人群中 HCV 感染者 HCV 基因分型及感染途徑研究. 中國輸血雜志, 2016, 29(1): 25-28.
10. Taherkhani R, Farshadpour F. Epidemiology of hepatitis C virus in Iran. World J Gastroenterol, 2015, 21(38): 10790-10810.
11. Triantos C, Konstantakis C, Tselekouni P, et al. Epidemiology of hepatitis C in Greece. World J Gastroenterol, 2016, 22(36): 8094-8102.
12. Daw MA, El-Bouzedi A, Ahmed MO, et al. Hepatitis C virus in North Africa: an emerging threat. Scientific World Journal, 2016, 2016: 1-11.
13. Sadeghi F, Salehi-Vaziri M, Almasi-Hashiani A, et al. Prevalence of Hepatitis C Virus Genotypes Among Patients in Countries of the Eastern Mediterranean Regional Office of WHO (EMRO) : A Systematic Review and Meta-Analysis. Hepat Mon, 2016, 16(4): e35558.
14. Petruzziello A, Marigliano S, Loquercio G, et al. Hepatitis C virus (HCV) genotypes distribution: an epidemiological up-date in Europe. Infect Agent Cancer, 2016, 11: 53.
15. Lim SG, Dan YY. A 2015 roadmap for the management of hepatitis C virus infections in Asia. Korean J Intern Med, 2015, 30(4): 423-433.
16. Chen W, Liao B, Hu F, et al. Changing Epidemiology of Hepatitis C Virus Genotype among Patients with Human Immunodeficiency Virus/Hepatitis C Virus Co-Infection in China. PLoS One, 2016, 11(9): e161844.
17. 陳旭, 蘇明華, 江建寧, 等. 廣西地區 HIV 合并 HCV 感染人群 HCV 基因型分布特征. 中國艾滋病性病, 2016, 22(8): 593-596.
18. 張宇, 柳麗娟, 張啟云. 福建地區慢性丙型肝炎患者丙型肝炎病毒基因分型研究. 福建醫藥雜志, 2016, 38(2): 91-94.
19. Wu Z, Cui L, Zhao W, et al. Molecular epidemiology of hepatitis C infections in Ningxia, China: genotype, phylogeny and mutation analysis. Virol J, 2016, 13(1): 172.
20. 肖桂榮, 徐珽. 2009～2013 年四川大學華西醫院 1 998 株丙型肝炎病毒基因分型研究. 四川大學學報(醫學版), 2016, 47(5): 755-758.
21. Vilibic-Cavlek T, Kucinar J, Kaic B, et al. Epidemiology of hepatitis C in Croatia in the European context. World J Gastroenterol, 2015, 21(32): 9476-9493.
22. Wan Z, Chen Q, Chen X, et al. HCV Diversity among Chinese and Burmese IDUs in Dehong, Yunnan, China. PLoS One, 2016, 11(9): e163062.
23. Yang L, Jiang C, Hu S, et al. Evolving Diversity of Hepatitis C Viruses in Yunnan Honghe, China. Int J Mol Sci, 2016, 17(3): 403.
24. Wu T, Xiong L, Wang F, et al. A Unique Pattern of HCV Genotype Distribution on Hainan Island in China Revealed by Evolutionary Analysis. Cell Physiol Biochem, 2016, 39(1): 316-330.
25. Niu Z, Zhang P, Tong Y. Age and gender distribution of Hepatitis C virus prevalence and genotypes of individuals of physical examination in WuHan, Central China. Springerplus, 2016, 5(1): 1557.
26. 丁柳, 鐘慧鈺, 宋興勃, 等. 四川人群丙肝病毒基因型分布及與抗病毒療效的相關性. 四川大學學報（醫學版）, 2015, 46(4): 573-577.
27. 林江, 溫先勇, 唐敏. 川南地區漢族人群丙型肝炎病毒基因分型及其與臨床關系. 檢驗醫學與臨床, 2016, 13(10): 1359-1361.
28. Freeman AJ, Dore GJ, Law MG, et al. Estimating progression to cirrhosis in chronic hepatitis C virus infection. Hepatology, 2001, 34(4 Pt 1): 809-816.
29. Li J F, Liu S, Ren F, et al. Fibrosis progression in interferon treatment-naive Chinese plasma donors with chronic hepatitis C for 20 years: a cohort study. Int J Infect Dis, 2014, 27: 49-53.
30. Rao H Y, Sun D G, Yang R F, et al. Outcome of hepatitis C virus infection in Chinese paid plasma donors: a 12-19-year cohort study. J Gastroenterol Hepatol, 2012, 27(3): 526-532.
31. Kenny-Walsh E. Clinical outcomes after hepatitis C infection from contaminated anti-D immune globulin. Irish Hepatology Research Group. N Engl J Med, 1999, 340(16): 1228-1233.
32. El-Serag HB, Rudolph KL. Hepatocellular carcinoma: epidemiology and molecular carcinogenesis. Gastroenterology, 2007, 132(7): 2557-2576.
33. Yan LB, Rao HY, Ma YJ, et al. Hepatitis B virus infection in Chinese patients with hepatitis C virus infection: prevalence, clinical characteristics, viral interactions and host genotypes: a nationwide cross-sectional study. BMJ Open, 2016, 6(10): e12016.
34. Simmonds P, Bukh J, Combet C, et al. Consensus proposals for a unified system of nomenclature of hepatitis C virus genotypes. Hepatology, 2005, 42(4): 962-973.
35. Bouchardeau F, Cantaloube J F, Chevaliez S, et al. Improvement of hepatitis C virus (HCV) genotype determination with the new version of the INNO-LiPA HCV assay. J Clin Microbiol, 2007, 45(4): 1140-1145.
36. Yang R, Cong X, Du S, et al. Performance comparison of the versant HCV genotype 2.0 assay (LiPA) and the abbott realtime HCV genotype II assay for detecting hepatitis C virus genotype 6. J Clin Microbiol, 2014, 52(10): 3685-3692.
37. 楊靜靜. 丙型肝炎病毒基因型及 F 蛋白與丙型肝炎慢性化、肝癌細胞凋亡的關系研究. 南京: 南京醫科大學, 2009.
38. Cui Y, Jia J. Update on epidemiology of hepatitis B and C in China. J Gastroenterol Hepatol, 2013, 28 (Suppl 1): 7-10.
39. Silini E, Bono F, Cerino A, et al. Virological features of hepatitis C virus infection in hemodialysis patients. J Clin Microbiol, 1993, 31(11): 2913-2917.
40. Janjua NZ, Chong M, Kuo M, et al. Long-term effect of sustained virological response on hepatocellular carcinoma in patients with hepatitis C in Canada. J Hepatol, 2017, 66(3)504-513.
41. Zaltron S, Spinetti A, Biasi L, et al. Chronic HCV infection: epidemiological and clinical relevance. BMC Infect Dis, 2012,12(Suppl 2): S2.
42. Chemaitelly H, Chaabna K, Abu-Raddad LJ. The Epidemiology of Hepatitis C Virus in the Fertile Crescent: Systematic Review and Meta-Analysis. PLoS One, 2015, 10(8): e135281.

Chinese Journal of Evidence-Based Medicine

The investigation of genotypes of HCV and clinical characteristics in HCV patients in West China Hospital of Sichuan University

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