Development of Animal Burn Models in Rats: A Systematic Review_Chinese Journal of Evidence-Based Medicine

Authors：

 WUBin ¹ ^# ,  TIANFang-yuan ² ^# , ZHANMei ¹ ,  XUTing ¹

1. Department of Pharmacy, West China Hospital, Sichuan University, Chengdu 610041, China;
2. West China School of Pharmacy, Sichuan University, Chengdu 610041, China;

Corresponding?author：

XUTing, Email: tingx2009@163.com

Keywords：

Rats; Burn; Disease model; Systematic review

DOI：

10.7507/1672-2531.20160204

Video：

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Objective To assess the methods, processes and evaluation criteria of burn models in rats. Methods Databases including MEDLINE, EMbase, CBM, CNKI, VIP and WanFang Data were electronically searched from inception to February 2016. The animal studies of burn models development in rats were included. Two reviewers independently screened literatures, extracted data, assessed the quality of included studies, and analyzed the outcomes. Results Fifteen studies including 38 burn groups were identified. The results indicated:(1) depth of burn:50.00% burn models were partial thickness burn (II°), and 21.05% were full thickness burn (III°). (2) methods of induction:80.00% burn models were developed through high temperature liquid or solid tools. (3) burn sites:93.33% burns were on the back of rats. (4) induction temperature:66.67% induction temperature was between 80℃ and 100℃. (5) induction time:86.67% induction time was between 3 seconds and 100 seconds. (6) anesthesia:40.00% rats were anesthetized with intraperitoneal injection of pentobarbital sodium. (7) skin preparation:60.00% studies reported the skin was prepared by physical and chemical methods. (8) housing post surgery:13.33% studies reported the housing temperature post surgery. (9) intervention post surgery:13.33% studies reported antishock treatment. (10) assessment criteria:almost all studies evaluated the depth of burn through the macroscopic and microcosmic assessment. Conclusion Based on current animal studies, most burn models in rats are partial thickness and full thickness burn on the back of rats. Burn is inducted mainly by 80℃ to 100℃ hot liquid or solid tools within 30 seconds, according with skin preparation, anesthesia, antishock or analgesia management. The depth of burn is evaluated by the macroscopic and microcosmic assessment. However, there is no standard for the methods, processes, assessment and reporting of development of burn model in rats.

Citation： WUBin, TIANFang-yuan, ZHANMei, XUTing. Development of Animal Burn Models in Rats: A Systematic Review. Chinese Journal of Evidence-Based Medicine, 2016, 16(11): 1354-1359. doi: 10.7507/1672-2531.20160204 Copy

1.	世界衛生組織燒傷實況報道第365號. 2016年9月. Avaliable at:http://www.who.int/mediacenter/factsheets/fs365/zh/.
2.	Mirastschijski U, Sander J, Weyand B, et al. Rehabilitation of burn patients:an underestimated socio-economic burden. Burns, 2013, 39(2):262-268.
3.	張立穎, 李亞潔, 揚磊, 等. Wistar大鼠Ⅱ度燙傷模型的建立.眼科新進展, 2010, 30(7):625-628.
4.	楊軍, 劉曉瑾.大鼠燙傷創面模型的制備和創面觀察.陜西醫學雜志, 2009, 38(7):794-795.
5.	Hooijmans C, Rovers MM, de Vries RB, et al. SYRCLE's risk of bias tool for animal studies. BMC Med Res Methodol, 2014, 5(14):43.
6.	Kistler D, Hafemann B, Schmidt K. A model to reproduce predictable full-thickness burns in an experimental animal. Burns Incl Therm Inj, 1988, 14(4):297-302.
7.	馮世杰, 花蘭女, 金曙雯, 等.大鼠燙傷模型的制作.上海第二醫科大學學報, 1995, 15(3):195-197.
8.	Bairy KL, Somayaji SN, Rao CM. An experimental model to produce partial thickness burn wound. Indian J Exp Biol, 1997, 35(1):70-72.
9.	Ma W, Huo C, Tian S, et al. An experimental investigation of deep burn by constant low heat. Zhonghua Shao Shang Za Zhi, 2002, 18(2):99-101.
10.	Becic F, Mornjakovic Z, Zulic I, et al. Animal model of thermal injuries. Bosn J Basic Med Sci, 2003, 3(4):41-46.
11.	姚慶君, 賈赤宇, 陳璧, 等.一種大鼠蒸氣燙傷模型的建立.中華燒傷雜志, 2004, 20(3):168-170.
12.	王年云, 李國輝.可控噴吸水燙傷大鼠體表模型的制作.中華燒傷雜志, 2006, 22(5):387-388.
13.	侯敏娜, 平大地.混合燃料建立大鼠火焰燒傷模型的實驗研究.云南中醫中藥雜志, 2010, 31(8):67-68.
14.	Gurfinkel R, Singer AJ, Cagnano E, et al. Development of a novel animal burn model using radiant heat in rats and swine. Acad Emerg Med, 2010, 17(5):514-520.
15.	遲云飛, 柴家科, 馬麗, 等.三維掃描技術精確控制大鼠燙傷面積動物模型的建立.中華損傷與修復雜志(電子版), 2011, 6(2):196-201.
16.	王雪妮, 張德芹, 黃云英, 等.大鼠燙傷模型制備的實驗研究.中醫藥臨床雜志, 2011, 30 (4):218-219.
17.	Tavares Pereira Ddos S, Lima-Ribeiro MH, de Pontes-Filho NT, et al. Development of animal model for studying deep second-degree thermal burns. J Biomed Biotechnol, 2012, 2012:460841.
18.	Cai EZ, Ang CH, Raju A, et al. Creation of Consistent Burn Wounds in a Rat Model. Arch Plast Surg, 2014, 41(4):317-324.

1. 世界衛生組織燒傷實況報道第365號. 2016年9月. Avaliable at:http://www.who.int/mediacenter/factsheets/fs365/zh/.
2. Mirastschijski U, Sander J, Weyand B, et al. Rehabilitation of burn patients:an underestimated socio-economic burden. Burns, 2013, 39(2):262-268.
3. 張立穎, 李亞潔, 揚磊, 等. Wistar大鼠Ⅱ度燙傷模型的建立.眼科新進展, 2010, 30(7):625-628.
4. 楊軍, 劉曉瑾.大鼠燙傷創面模型的制備和創面觀察.陜西醫學雜志, 2009, 38(7):794-795.
5. Hooijmans C, Rovers MM, de Vries RB, et al. SYRCLE's risk of bias tool for animal studies. BMC Med Res Methodol, 2014, 5(14):43.
6. Kistler D, Hafemann B, Schmidt K. A model to reproduce predictable full-thickness burns in an experimental animal. Burns Incl Therm Inj, 1988, 14(4):297-302.
7. 馮世杰, 花蘭女, 金曙雯, 等.大鼠燙傷模型的制作.上海第二醫科大學學報, 1995, 15(3):195-197.
8. Bairy KL, Somayaji SN, Rao CM. An experimental model to produce partial thickness burn wound. Indian J Exp Biol, 1997, 35(1):70-72.
9. Ma W, Huo C, Tian S, et al. An experimental investigation of deep burn by constant low heat. Zhonghua Shao Shang Za Zhi, 2002, 18(2):99-101.
10. Becic F, Mornjakovic Z, Zulic I, et al. Animal model of thermal injuries. Bosn J Basic Med Sci, 2003, 3(4):41-46.
11. 姚慶君, 賈赤宇, 陳璧, 等.一種大鼠蒸氣燙傷模型的建立.中華燒傷雜志, 2004, 20(3):168-170.
12. 王年云, 李國輝.可控噴吸水燙傷大鼠體表模型的制作.中華燒傷雜志, 2006, 22(5):387-388.
13. 侯敏娜, 平大地.混合燃料建立大鼠火焰燒傷模型的實驗研究.云南中醫中藥雜志, 2010, 31(8):67-68.
14. Gurfinkel R, Singer AJ, Cagnano E, et al. Development of a novel animal burn model using radiant heat in rats and swine. Acad Emerg Med, 2010, 17(5):514-520.
15. 遲云飛, 柴家科, 馬麗, 等.三維掃描技術精確控制大鼠燙傷面積動物模型的建立.中華損傷與修復雜志(電子版), 2011, 6(2):196-201.
16. 王雪妮, 張德芹, 黃云英, 等.大鼠燙傷模型制備的實驗研究.中醫藥臨床雜志, 2011, 30 (4):218-219.
17. Tavares Pereira Ddos S, Lima-Ribeiro MH, de Pontes-Filho NT, et al. Development of animal model for studying deep second-degree thermal burns. J Biomed Biotechnol, 2012, 2012:460841.
18. Cai EZ, Ang CH, Raju A, et al. Creation of Consistent Burn Wounds in a Rat Model. Arch Plast Surg, 2014, 41(4):317-324.

Chinese Journal of Evidence-Based Medicine

Development of Animal Burn Models in Rats: A Systematic Review

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