Effects of Short-term Fertilization versus Conventional Fertilization in Vitro Fertilization Cycles: A Meta-Analysis_Chinese Journal of Evidence-Based Medicine

Authors：

 JIANQi-liang ¹ ,  WANGFang ¹ , DINGNan ² , DUHong ¹ , HUANGShan ¹

1. Department of Reproductive Medicine, Second Hospital of Lanzhou University, Lanzhou 730030, China;
2. Second Medical College of Lanzhou University, Lanzhou 730030, China;

Corresponding?author：

WANGFang, Email: wangfang4323@126.com

Keywords：

In vitro fertilization-embryo transfer; Short-term fertilization; Conventional fertilization; Systematic review; Meta-analysis; Randomized controlled trial

DOI：

10.7507/1672-2531.20140240

Video：

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Objective To systematically review the clinical effects of short-term and conventional fertilization for vitro fertilization-embryo transfer (IVF-ET). Methods Randomized controlled trials (RCTs) about the clinical effects of short-term fertilization versus conventional fertilization for IVF-ET were searched in PubMed, The Cochrane Library (Issue 8, 2014), CBM, CNKI, WanFang Data and VIP from inception to August 2014. Two reviewers independently screened literature according to the inclusion and exclusion criteria, extracted data, and assessed methodological quality of included studies. Then meta-analysis was performed using RevMan 5.2 software. Results A total of six RCTs involving 1 373 patients were finally included. The results of meta-analysis indicated that:short-term fertilization was superior to conventional fertilization in increasing high quality embryo rates (OR=1.42, 95%CI 1.18 to 1.70, P=0.000 2) as well as clinical pregnancy rates (OR=1.67, 95%CI 1.33 to 2.09, P < 0.000 01). However, the two groups were alike in fertilization rates, polyspermy rates, and miscarriage rates. Conclusion Current evidence indicates that short-term fertilization is superior to conventional fertilization in increasing high quality embryo rates as well as clinical pregnancy rates. Due to limited quality and quantity of the included studies, the above conclusion should be verified by conducting more large-scale, high quality RCTs with long-term follow-up.

Citation： JIANQi-liang, WANGFang, DINGNan, DUHong, HUANGShan. Effects of Short-term Fertilization versus Conventional Fertilization in Vitro Fertilization Cycles: A Meta-Analysis. Chinese Journal of Evidence-Based Medicine, 2014, 14(12): 1504-1509. doi: 10.7507/1672-2531.20140240 Copy

1.	李力.實用生殖醫學.北京:人民衛生出版社, 2012:209.
2.	李曉紅, 王蒿, 張曉, 等.短時受精與卵胞漿內單精子注射的臨床應用研究.中華泌尿外科雜志, 2004, 25(7):485-487.
3.	黃國寧, 韓偉, 國玉蕊.短時受精與"搭橋受精".生殖醫學雜志, 2009, 18(3):180-183.
4.	Dirnfeld M, Bider D, Koifman M, et al. Shortened exposure of oocytes to spermatozoa improve in vitro fertilization outcome:a Prospective randomized controlled study. Hum Reprod, 1999, 14(11):2562-2564.
5.	Barraud-Lange V, Sifer C, Pocate K, et al. Short gamete coincubation duringinvitro fertilization decreases the fertilization rateand does not imProveembryo quality:aprospectiveauto controlled study. J Assist Reprod Genet, 2008, 25(7):305-310.
6.	張寧媛, 孫海翔, 胡婭莉, 等.短時受精聯合早期補救性卵細胞胞質內單精子注射在完全受精失敗周期的臨床應用, 中華男科學雜志, 2009, 15(6):538-541.
7.	李豫峰, 鎮春, 何巧花, 等.體外受精-胚胎移植患者妊娠率與年齡及多精受精關系的研究.中國優生與遺傳雜志, 2008, 16(1):102-103.
8.	李媛.人類輔助生殖實驗技術.北京:科學出版社, 2008:100.
9.	Higgins JP, Green S. Cochrane Handbook for Systematic Reviews of Interventions (Version 5.1.0).[cited 2012 Jan 5]. Available at: http://www.cochrane-handbook.org.
10.	Gianaroli L, Cristina Magli M, Magli MC, et al. Reducing the time of sperm-oocyte interaction in human in·vitro fertilization improves the implantation rate. Hum ReProd, 1996, 11(1):166-171.
11.	Kattera S, Chen C. Short coincubation of gametes in in-vitro fertilization improves implantation and Pregnancyrates:a prospective, randomized, controlled study. Fertil Steril, 2003, 80(4):1017-1021.
12.	刁英, 楊智敏, 譚兵兵, 等.短時受精在體外受精-胚胎移植治療周期中的應用價值.中國婦幼保健, 2010, 25(15):2104-2106.
13.	李瑞岐, 王文軍, 黃麗麗, 等.短時受精對體外受精妊娠結局的影響.中山大學學報, 2012, 33(5):683-686.
14.	張若鵬, 邵華, 張麗蓉, 等.短時受精對體外受精-胚胎移植胚胎質量與妊娠率的影響.生殖醫學雜志, 2004, 13(1):44-46.
15.	Harraway C, Berger NG, Dubin NH, et al. Semen PH in patients with normal versus abnormal sperm characteristics. Am J Obstet Gyneco, 2000, 182:1045-1047.
16.	羅麗蘭.不孕與不育.北京:人民衛生出版社, 1999:418-423.
17.	Lundqvist M, Johansson U, Lundkvist O, et al. Reducing the time of co-incubation of gametes in human in vitro fertilization has no beneficial effect. Reprod Biomed Online, 2001, 3(1):21-24.
18.	Enkhmaa D, Kasai T, Hoshi K. Long-time exposure of mouse embryos to the sperm produces high levels of reactive oxygen species in culture medium and relates to poor embryo development. Reprod Domest Anim, 2009, 44(4):634-637.
19.	Navarro C, LoPez E, Stone B, et al. Short coincubation of sperm with oocytes is associated with an increased incidence of Polyspermy in human in vitro fertilization (IVF). Fertil Steril, 2004, 82(Suppl 2):S325-S325.
20.	樂杰, 主編.婦產科學.第5版, 北京:人民衛生出版社, 2002:34-35.
21.	李蕾, 李尚為, 靳松, 等.應用顆粒細胞部分剝除法提高行體外受精-胚胎移植后胚胎質量及臨床妊娠率的研究.中華婦產科雜志, 2006, 41(3):201-202.
22.	Bedaiwy MA, Falcone T, Mohamed MS, et al. Differential growth of human embryos in vitro:role of reactive oxygen species. Fertil Steril, 2004, 82(3):593-600.
23.	MenezoY, Barak Y. Comparison between day 2 embryos obtained either from ICSI or Resulting from short insemination IVF:influence of maternal age. Hum Reprod, 2000, 15(9):1776-1780.
24.	Shau PL, Robertkuo KL, Jin TS, et al. The effects of brief gamete coincubation in human in vitro fertilization. J Assist Reprod Gen, 2000, 17(6):344-348.
25.	Swenson K, Check JH, Summers D, et al. A randomized study comparing the effect of standard verse short incubation of sperm and occyte on subsequent pregnancy and implantation rates following in vitro fertilization embryo transfer. Arch Androl, 2000, 45(1):73-76.
26.	柴三明, 劉珍, 倪亞莉, 等.體外受精中精卵孵育時間對結局的影響.中國優生與遺傳雜志, 2006, 14(10):83-84.
27.	Dirnfeld M, Shiloh H, Brder E. et al. A prospective randomized controlled study of the effect of short coincubation of gametes during insemination on Zona pellucida thickness. Gynecol Endocrinol, 2003, 17(5):397-403.
28.	肖勁松, 陳雙隕, 張春蓮, 等.促性腺激素釋放激素拮抗劑在體外受精-胚胎移植中有效性的系統性評價.中國循證醫學雜志, 2010, 10(12):1432-1438.

1. 李力.實用生殖醫學.北京:人民衛生出版社, 2012:209.
2. 李曉紅, 王蒿, 張曉, 等.短時受精與卵胞漿內單精子注射的臨床應用研究.中華泌尿外科雜志, 2004, 25(7):485-487.
3. 黃國寧, 韓偉, 國玉蕊.短時受精與"搭橋受精".生殖醫學雜志, 2009, 18(3):180-183.
4. Dirnfeld M, Bider D, Koifman M, et al. Shortened exposure of oocytes to spermatozoa improve in vitro fertilization outcome:a Prospective randomized controlled study. Hum Reprod, 1999, 14(11):2562-2564.
5. Barraud-Lange V, Sifer C, Pocate K, et al. Short gamete coincubation duringinvitro fertilization decreases the fertilization rateand does not imProveembryo quality:aprospectiveauto controlled study. J Assist Reprod Genet, 2008, 25(7):305-310.
6. 張寧媛, 孫海翔, 胡婭莉, 等.短時受精聯合早期補救性卵細胞胞質內單精子注射在完全受精失敗周期的臨床應用, 中華男科學雜志, 2009, 15(6):538-541.
7. 李豫峰, 鎮春, 何巧花, 等.體外受精-胚胎移植患者妊娠率與年齡及多精受精關系的研究.中國優生與遺傳雜志, 2008, 16(1):102-103.
8. 李媛.人類輔助生殖實驗技術.北京:科學出版社, 2008:100.
9. Higgins JP, Green S. Cochrane Handbook for Systematic Reviews of Interventions (Version 5.1.0).[cited 2012 Jan 5]. Available at: http://www.cochrane-handbook.org.
10. Gianaroli L, Cristina Magli M, Magli MC, et al. Reducing the time of sperm-oocyte interaction in human in·vitro fertilization improves the implantation rate. Hum ReProd, 1996, 11(1):166-171.
11. Kattera S, Chen C. Short coincubation of gametes in in-vitro fertilization improves implantation and Pregnancyrates:a prospective, randomized, controlled study. Fertil Steril, 2003, 80(4):1017-1021.
12. 刁英, 楊智敏, 譚兵兵, 等.短時受精在體外受精-胚胎移植治療周期中的應用價值.中國婦幼保健, 2010, 25(15):2104-2106.
13. 李瑞岐, 王文軍, 黃麗麗, 等.短時受精對體外受精妊娠結局的影響.中山大學學報, 2012, 33(5):683-686.
14. 張若鵬, 邵華, 張麗蓉, 等.短時受精對體外受精-胚胎移植胚胎質量與妊娠率的影響.生殖醫學雜志, 2004, 13(1):44-46.
15. Harraway C, Berger NG, Dubin NH, et al. Semen PH in patients with normal versus abnormal sperm characteristics. Am J Obstet Gyneco, 2000, 182:1045-1047.
16. 羅麗蘭.不孕與不育.北京:人民衛生出版社, 1999:418-423.
17. Lundqvist M, Johansson U, Lundkvist O, et al. Reducing the time of co-incubation of gametes in human in vitro fertilization has no beneficial effect. Reprod Biomed Online, 2001, 3(1):21-24.
18. Enkhmaa D, Kasai T, Hoshi K. Long-time exposure of mouse embryos to the sperm produces high levels of reactive oxygen species in culture medium and relates to poor embryo development. Reprod Domest Anim, 2009, 44(4):634-637.
19. Navarro C, LoPez E, Stone B, et al. Short coincubation of sperm with oocytes is associated with an increased incidence of Polyspermy in human in vitro fertilization (IVF). Fertil Steril, 2004, 82(Suppl 2):S325-S325.
20. 樂杰, 主編.婦產科學.第5版, 北京:人民衛生出版社, 2002:34-35.
21. 李蕾, 李尚為, 靳松, 等.應用顆粒細胞部分剝除法提高行體外受精-胚胎移植后胚胎質量及臨床妊娠率的研究.中華婦產科雜志, 2006, 41(3):201-202.
22. Bedaiwy MA, Falcone T, Mohamed MS, et al. Differential growth of human embryos in vitro:role of reactive oxygen species. Fertil Steril, 2004, 82(3):593-600.
23. MenezoY, Barak Y. Comparison between day 2 embryos obtained either from ICSI or Resulting from short insemination IVF:influence of maternal age. Hum Reprod, 2000, 15(9):1776-1780.
24. Shau PL, Robertkuo KL, Jin TS, et al. The effects of brief gamete coincubation in human in vitro fertilization. J Assist Reprod Gen, 2000, 17(6):344-348.
25. Swenson K, Check JH, Summers D, et al. A randomized study comparing the effect of standard verse short incubation of sperm and occyte on subsequent pregnancy and implantation rates following in vitro fertilization embryo transfer. Arch Androl, 2000, 45(1):73-76.
26. 柴三明, 劉珍, 倪亞莉, 等.體外受精中精卵孵育時間對結局的影響.中國優生與遺傳雜志, 2006, 14(10):83-84.
27. Dirnfeld M, Shiloh H, Brder E. et al. A prospective randomized controlled study of the effect of short coincubation of gametes during insemination on Zona pellucida thickness. Gynecol Endocrinol, 2003, 17(5):397-403.
28. 肖勁松, 陳雙隕, 張春蓮, 等.促性腺激素釋放激素拮抗劑在體外受精-胚胎移植中有效性的系統性評價.中國循證醫學雜志, 2010, 10(12):1432-1438.

Chinese Journal of Evidence-Based Medicine

Effects of Short-term Fertilization versus Conventional Fertilization in Vitro Fertilization Cycles: A Meta-Analysis

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